SCD1 Antibody - #DF13253

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产品: SCD1 抗体
货号: DF13253
描述: Rabbit polyclonal antibody to SCD1
应用: WB IHC IF/ICC
反应: Human, Mouse
预测: Pig, Bovine, Sheep, Dog
分子量: 41kDa; 42kD(Calculated).
蛋白号: O00767
RRID: AB_2846272

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Pig(100%), Bovine(100%), Sheep(100%), Dog(100%)
克隆:
Polyclonal
特异性:
SCD1 Antibody detects endogenous levels of total SCD1.
RRID:
AB_2846272
引用格式: Affinity Biosciences Cat# DF13253, RRID:AB_2846272.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ACOD_HUMAN; Acyl-CoA desaturase; Delta(9)-desaturase; Delta-9 desaturase; Delta-9-Desaturase; FADS5; Fatty acid desaturase; PRO0998; SCD 1; SCD; SCD1; Stearoyl-CoA desaturase;

抗原和靶标

免疫原:
Uniprot:

O00767(ACOD_HUMAN) >>Visit HPA database.

基因/基因ID:
SCD(6319)
表达:
O00767 ACOD_HUMAN:

Detected in fetal liver, lung and brain. Highly expressed in adult adipose tissue, and at lower levels in adult brain and lung.

序列:
MPAHLLQDDISSSYTTTTTITAPPSRVLQNGGDKLETMPLYLEDDIRPDIKDDIYDPTYKDKEGPSPKVEYVWRNIILMSLLHLGALYGITLIPTCKFYTWLWGVFYYFVSALGITAGAHRLWSHRSYKARLPLRLFLIIANTMAFQNDVYEWARDHRAHHKFSETHADPHNSRRGFFFSHVGWLLVRKHPAVKEKGSTLDLSDLEAEKLVMFQRRYYKPGLLMMCFILPTLVPWYFWGETFQNSVFVATFLRYAVVLNATWLVNSAAHLFGYRPYDKNISPRENILVSLGAVGEGFHNYHHSFPYDYSASEYRWHINFTTFFIDCMAALGLAYDRKKVSKAAILARIKRTGDGNYKSG

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Horse
57
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - O00767 作为底物

Site PTM Type Enzyme
Y14 Phosphorylation
K34 Ubiquitination
Y41 Phosphorylation
K51 Sumoylation
K51 Ubiquitination
Y55 Phosphorylation
Y59 Phosphorylation
K60 Ubiquitination
K62 Ubiquitination
K68 Sumoylation
K68 Ubiquitination
S80 Phosphorylation
T91 Phosphorylation
T95 Phosphorylation
K162 Ubiquitination
K189 Ubiquitination
K194 Ubiquitination
K196 Ubiquitination
S198 Phosphorylation
T199 Phosphorylation
S203 Phosphorylation
K209 Ubiquitination
K278 Ubiquitination
K338 Ubiquitination
K341 Ubiquitination
T351 Phosphorylation
K357 Ubiquitination

研究背景

功能:

Stearyl-CoA desaturase that utilizes O(2) and electrons from reduced cytochrome b5 to introduce the first double bond into saturated fatty acyl-CoA substrates. Catalyzes the insertion of a cis double bond at the delta-9 position into fatty acyl-CoA substrates including palmitoyl-CoA and stearoyl-CoA. Gives rise to a mixture of 16:1 and 18:1 unsaturated fatty acids. Plays an important role in lipid biosynthesis. Plays an important role in regulating the expression of genes that are involved in lipogenesis and in regulating mitochondrial fatty acid oxidation (By similarity). Plays an important role in body energy homeostasis (By similarity). Contributes to the biosynthesis of membrane phospholipids, cholesterol esters and triglycerides (By similarity).

细胞定位:

Endoplasmic reticulum membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Detected in fetal liver, lung and brain. Highly expressed in adult adipose tissue, and at lower levels in adult brain and lung.

亚基结构:

May self-associate and form homodimers.

蛋白家族:

The histidine box domains are involved in binding the catalytic metal ions.

Belongs to the fatty acid desaturase type 1 family.

研究领域

· Environmental Information Processing > Signal transduction > AMPK signaling pathway.   (View pathway)

· Metabolism > Lipid metabolism > Biosynthesis of unsaturated fatty acids.

· Metabolism > Global and overview maps > Fatty acid metabolism.

· Organismal Systems > Endocrine system > PPAR signaling pathway.

文献引用

1). Metabolomics reveals that CAF-derived lipids promote colorectal cancer peritoneal metastasis by enhancing membrane fluidity. International Journal of Biological Sciences, 2022 (PubMed: 35342344) [IF=9.2]

Application: WB    Species: human    Sample: DLD1 and HCT116 cells

Figure 5. |Low expression of SCD is associated with poor prognosis in patients with CRC and PM-CRC. A, Schematic representation of fatty acid synthesis and function. B, Western blot showing SCD expression in DLD1 and HCT116 cells incubated with CAF-CM.

Application: IHC    Species: human    Sample: DLD1 and HCT116 cells

Figure 5. |Low expression of SCD is associated with poor prognosis in patients with CRC and PM-CRC. C, RT-qPCR was performed to measure the mRNA level of SCD in DLD1/HCT116 cells incubated with CAF-CM. D-E, Comparison of SCD protein expression by IHC (D).
2). AKAP8L enhances the stemness and chemoresistance of gastric cancer cells by stabilizing SCD1 mRNA. Cell Death & Disease, 2022 (PubMed: 36522343) [IF=9.0]
3). 1,8-cineole ameliorates experimental diabetic angiopathy by inhibiting NLRP3 inflammasome-mediated pyroptosis in HUVECs via SIRT2. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 2024 (PubMed: 38972150) [IF=7.5]

Application: WB    Species: Mouse    Sample:

Fig. 2. 1,8-cineole decreases the expression of pyroptosis-related proteins in the thoracic aorta of DM mice. (A, B) Expression of the protein SIRT2 associated with pyroptosis in the thoracic aorta of diabetic mice was assessed using western blotting. (C, D) Effect of 1,8-cineole on the levels of IL-18 and IL-1β in murine serum. (E) Effect of 1,8-cineole on the concentration of lactate dehydrogenase (LDH) in mouse serum. Results are presented as the mean ± SD (n = 6), *p < 0.05, **p < 0.01 versus control group; #p < 0.05, ##p < 0.01 versus DM group.
4). Yogurt-derived Lactobacillus plantarum Q16 alleviated high-fat diet-induced non-alcoholic fatty liver disease in mice. Food Science and Human Wellness, 2022 [IF=7.0]

Application: WB    Species: Mouse    Sample:

Fig. 5. Effects ofL. plantarum Q16 on key proteins involved in hepatic lipid metabolism in HFD-fed obese mice. Data are presented as mean ± SD (n = 6). Different lowercase alphabet letters were significantly different at the level of P < 0.05.
5). Peptides released from bovine α-lactalbumin by simulated digestion alleviated free fatty acids-induced lipid accumulation in HepG2 cells. Journal of Functional Foods, 2021 [IF=5.6]
6). Leptin Silencing Attenuates Lipid Accumulation through Sterol Regulatory Element-Binding Protein 1 Inhibition in Nasopharyngeal Carcinoma. International journal of molecular sciences, 2022 (PubMed: 35628510) [IF=5.6]

Application: IHC    Species: Mouse    Sample:

Figure 5. Leptin inhibition attenuates SREBP1 expression in vivo. (A) Mouse body weights were measured in shcontrol and shleptin groups. (B) IHC was performed to investigate the protein expression levels of SREBP1, FASN, and SCD1 in shcontrol and shleptin xenograft tumors. The quantifications of IHC staining are shown. The data are presented as the mean ± SD. ***: p < 0.001; n.s.: not significant.
7). Patchouli alcohol ameliorates acute liver injury via inhibiting oxidative stress and gut-origin LPS leakage in rats. International Immunopharmacology, 2021 (PubMed: 34182243) [IF=5.6]
8). Digeda-4 decoction and its disassembled prescriptions improve dyslipidemia and apoptosis by regulating AMPK/SIRT1 pathway on tyloxapol-induced nonalcoholic fatty liver disease in mice. Journal of Ethnopharmacology, 2023 (PubMed: 37348794) [IF=5.4]
9). Essential oil from Fructus Alpiniae zerumbet ameliorates vascular endothelial cell senescence in diabetes by regulating PPAR-γ signalling: A 4D label-free quantitative proteomics and network pharmacology study. Journal of ethnopharmacology, 2024 (PubMed: 38065350) [IF=5.4]
10). Sex hormone-binding globulin improves lipid metabolism and reduces inflammation in subcutaneous adipose tissue of metabolic syndrome-affected horses. Frontiers in molecular biosciences, 2023 (PubMed: 38146533) [IF=5.0]

Application: WB    Species: Human    Sample: adipose tissue

FIGURE 8 Influence of SHBG treatment of lipid metabolism related modulators interplay in SAT (A–N). The relative expression levels of LPL (A), SCD (B), PNPLA2 (C), PLIN1 (D), PPARA (E), FASN (F) in SAT were analyzed by RT-PCR. Protein levels of LPL (G), PLIN1 (H), FASN (I), SCD1 (J), ATGL (K, L) and HSL (M) were analyzed by Western blot ((N), representative membranes). Representative data are shown as mean ± SD.
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