产品: FCER1G 抗体
货号: DF13263
描述: Rabbit polyclonal antibody to FCER1G
应用: WB IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Dog
分子量: 9kDa; 10kD(Calculated).
蛋白号: P30273
RRID: AB_2846282

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   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%)
克隆:
Polyclonal
特异性:
FCER1G Antibody detects endogenous levels of total FCER1G.
RRID:
AB_2846282
引用格式: Affinity Biosciences Cat# DF13263, RRID:AB_2846282.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Fc fragment of IgE, high affinity I, receptor for; gamma polypeptide; Fc receptor gamma chain; Fc-epsilon RI-gamma; FCER1G; FCERG_HUMAN; FceRI gamma; FCRG; FcRgamma; High affinity immunoglobulin epsilon receptor subunit gamma; IgE Fc receptor subunit gamma; Immunoglobulin E receptor, high affinity, gamma chain;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
序列:
MIPAVVLLLLLLVEQAAALGEPQLCYILDAILFLYGIVLTLLYCRLKIQVRKAAITSYEKSDGVYTGLSTRNQETYETLKHEKPPQ

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P30273 作为底物

Site PTM Type Enzyme
Y58 Phosphorylation
K60 Ubiquitination
S61 Phosphorylation
Y65 Phosphorylation
T66 Phosphorylation
S69 Phosphorylation
T70 Phosphorylation
T75 Phosphorylation
Y76 Phosphorylation
T78 Phosphorylation

研究背景

功能:

Adapter protein containing an immunoreceptor tyrosine-based activation motif (ITAM) that transduces activation signals from various immunoreceptors. As a component of the high-affinity immunoglobulin E (IgE) receptor, mediates allergic inflammatory signaling in mast cells. As a constitutive component of interleukin-3 receptor complex, selectively mediates interleukin 4/IL4 production by basophils, priming T-cells toward effector T-helper 2 subset. Associates with pattern recognition receptors CLEC4D and CLEC4E to form a functional signaling complex in myeloid cells. Binding of mycobacterial trehalose 6,6'-dimycolate (TDM) to this receptor complex leads to phosphorylation of ITAM, triggering activation of SYK, CARD9 and NF-kappa-B, consequently driving maturation of antigen-presenting cells and shaping antigen-specific priming of T-cells toward effector T-helper 1 and T-helper 17 cell subtypes. May function cooperatively with other activating receptors. Functionally linked to integrin beta-2/ITGB2-mediated neutrophil activation. Also involved in integrin alpha-2/ITGA2-mediated platelet activation.

细胞定位:

Cell membrane>Single-pass type I membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

IgE Fc receptor is a tetramer of an alpha chain, a beta chain, and two disulfide-linked gamma chains. Associates with FCGR1A; forms a functional signaling complex. Associated with CLEC6A (By similarity). Interacts with CLEC4E. Interacts (via ITAM domain) with SYK (via SH2 domains); activates SYK, enabling integrin-mediated activation of neutrophils and macrophages (By similarity). Interacts with CSF2RB and recruits SYK in response to IL3 stimulation; this interaction is direct (By similarity). Interacts with CD300LH; the interaction may be indirect (By similarity). Interacts with CD300LD (By similarity). Interacts with TARM1.

蛋白家族:

Belongs to the CD3Z/FCER1G family.

研究领域

· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Immune diseases > Asthma.

· Organismal Systems > Immune system > Platelet activation.   (View pathway)

· Organismal Systems > Immune system > Natural killer cell mediated cytotoxicity.   (View pathway)

· Organismal Systems > Immune system > Fc epsilon RI signaling pathway.   (View pathway)

文献引用

1). Comprehensive landscape of immune-based classifier related to early diagnosis and macrophage M1 in spinal cord injury. Aging (Albany NY), 2023 (PubMed: 36842142) [IF=5.2]

Application: WB    Species: Rat    Sample:

Figure 10 Verification of the expression of Core IRGs via rat SCI model. (A, B) Sequencing results of Core IRGs in SCI and sham group; (C) WB analysis demonstrating the expression of Core IRGs in the sham, SCI-3d and 7d group, respectively; (D, E) Immunofluorescence showed the expression of Core IRGs in sham and SCI-7d groups. Bar = 50 um; *p < 0.05; **p < 0.01; and ***p < 0.001.

Application: IF/ICC    Species: Rat    Sample:

Figure 10 Verification of the expression of Core IRGs via rat SCI model. (A, B) Sequencing results of Core IRGs in SCI and sham group; (C) WB analysis demonstrating the expression of Core IRGs in the sham, SCI-3d and 7d group, respectively; (D, E) Immunofluorescence showed the expression of Core IRGs in sham and SCI-7d groups. Bar = 50 um; *p < 0.05; **p < 0.01; and ***p < 0.001.

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