产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF13300, RRID:AB_2846319.
展开/折叠
Core histone macro h2a.1; Core histone macro-H2A.1; H2A histone family member Y; H2A.y; H2A/y; H2AF12M; H2AFJ; H2afy; H2AY_HUMAN; Histone H2A.Y; Histone macroH2A1; Histone macroH2A1.1; Histone macroH2A1.2; Macroh2a1; MACROH2A1.1; MacroH2A1.2; Medulloblastoma antigen MU MB 50.205; Medulloblastoma antigen MU-MB-50.205; mH2a; mH2A1;
抗原和靶标
- O75367 H2AY_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSSRGGKKKSTKTSRSAKAGVIFPVGRMLRYIKKGHPKYRIGVGAPVYMAAVLEYLTAEILELAGNAARDNKKGRVTPRHILLAVANDEELNQLLKGVTIASGGVLPNIHPELLAKKRGSKGKLEAIITPPPAKKAKSPSQKKPVSKKAGGKKGARKSKKKQGEVSKAASADSTTEGTPADGFTVLSTKSLFLGQKLNLIHSEISNLAGFEVEAIINPTNADIDLKDDLGNTLEKKGGKEFVEAVLELRKKNGPLEVAGAAVSAGHGLPAKFVIHCNSPVWGADKCEELLEKTVKNCLALADDKKLKSIAFPSIGSGRNGFPKQTAAQLILKAISSYFVSTMSSSIKTVYFVLFDSESIGIYVQEMAKLDAN
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O75367 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K7 | Acetylation | Uniprot | |
| K12 | Acetylation | Uniprot | |
| K18 | Acetylation | Uniprot | |
| K18 | Methylation | Uniprot | |
| K18 | Ubiquitination | Uniprot | |
| R27 | Methylation | Uniprot | |
| Y55 | Phosphorylation | Uniprot | |
| K96 | Ubiquitination | Uniprot | |
| S102 | Phosphorylation | Uniprot | |
| K116 | Ubiquitination | Uniprot | |
| K117 | Ubiquitination | Uniprot | |
| S120 | Phosphorylation | Uniprot | |
| K121 | Ubiquitination | Uniprot | |
| K123 | Acetylation | Uniprot | |
| K123 | Methylation | Uniprot | |
| K123 | Ubiquitination | Uniprot | |
| T129 | Phosphorylation | Uniprot | |
| S138 | Phosphorylation | Uniprot | |
| S140 | Phosphorylation | Uniprot | |
| S146 | Phosphorylation | Uniprot | |
| K153 | Acetylation | Uniprot | |
| S158 | Phosphorylation | Uniprot | |
| K159 | Acetylation | Uniprot | |
| S166 | Phosphorylation | Uniprot | |
| K167 | Ubiquitination | Uniprot | |
| S170 | Phosphorylation | Uniprot | |
| S173 | Phosphorylation | Uniprot | |
| T174 | Phosphorylation | Uniprot | |
| T175 | Phosphorylation | Uniprot | |
| T178 | Phosphorylation | Uniprot | |
| T188 | Phosphorylation | Uniprot | |
| K189 | Ubiquitination | Uniprot | |
| K196 | Ubiquitination | Uniprot | |
| K235 | Ubiquitination | Uniprot | |
| K239 | Methylation | Uniprot | |
| R249 | Methylation | Uniprot | |
| K251 | Ubiquitination | Uniprot | |
| K271 | Ubiquitination | Uniprot | |
| S278 | Phosphorylation | Uniprot | |
| K285 | Acetylation | Uniprot | |
| K285 | Ubiquitination | Uniprot | |
| K292 | Acetylation | Uniprot | |
| K292 | Ubiquitination | Uniprot | |
| K295 | Ubiquitination | Uniprot | |
| K304 | Acetylation | Uniprot | |
| K304 | Ubiquitination | Uniprot | |
| K307 | Ubiquitination | Uniprot | |
| S308 | Phosphorylation | Uniprot | |
| S313 | Phosphorylation | Uniprot | |
| S316 | Phosphorylation | Uniprot | |
| K323 | Ubiquitination | Uniprot | |
| T325 | Phosphorylation | Uniprot | |
| K332 | Ubiquitination | Uniprot | |
| Y362 | Phosphorylation | Uniprot |
研究背景
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Involved in stable X chromosome inactivation. Inhibits the binding of transcription factors, including NF-kappa-B, and interferes with the activity of remodeling SWI/SNF complexes. Inhibits histone acetylation by EP300 and recruits class I HDACs, which induces a hypoacetylated state of chromatin.
Binds ADP-ribose and O-acetyl-ADP-ribose, and may be involved in ADP-ribose-mediated chromatin modulation. Increases the expression of genes involved in redox metabolism, including SOD3.
Represses SOD3 gene expression.
Monoubiquitinated at either Lys-116 or Lys-117. May also be polyubiquitinated. Ubiquitination is mediated by the CUL3/SPOP E3 complex and does not promote proteasomal degradation. Instead, it is required for enrichment in inactive X chromosome chromatin.
Nucleus. Chromosome.
Note: Enriched in inactive X chromosome chromatin and in senescence-associated heterochromatin.
Widely expressed.
The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. Interacts with HDAC1 and HDAC2 (By similarity). Interacts with SPOP. Part of a complex consisting of MACROH2A1, CUL3 and SPOP.
研究领域
· Cellular Processes > Cell growth and death > Necroptosis. (View pathway)
· Human Diseases > Substance dependence > Alcoholism.
· Human Diseases > Immune diseases > Systemic lupus erythematosus.
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