CXCL16 Antibody - #DF13312

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产品: CXCL16 抗体
货号: DF13312
描述: Rabbit polyclonal antibody to CXCL16
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
分子量: 27kDa, 45 kDa; 28kD(Calculated).
蛋白号: Q9H2A7
RRID: AB_2846331

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   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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MS Report
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
克隆:
Polyclonal
特异性:
CXCL16 Antibody detects endogenous levels of total CXCL16.
RRID:
AB_2846331
引用格式: Affinity Biosciences Cat# DF13312, RRID:AB_2846331.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

C-X-C motif chemokine 16; Chemokine (C X C motif) ligand 16; Chemokine, CXC motif, ligand 16; CXC chemokine ligand 16; Cxcl16; CXCLG16; CXL16_HUMAN; Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein; SCYB16; Small inducible cytokine B16 precursor; Small-inducible cytokine B16; SR-PSOX; SRPSOX; Transmembrane chemokine CXCL16; UNQ2759/PRO6714;

抗原和靶标

免疫原:
Uniprot:

Q9H2A7(CXL16_HUMAN) >>Visit HPA database.

基因/基因ID:
CXCL16(58191)
表达:
Q9H2A7 CXL16_HUMAN:

Expressed in T-cell areas. Expressed in spleen, lymph nodes, lung, kidney, small intestine and thymus. Weak expression in heart and liver and no expression in brain and bone marrow.

序列:
MGRDLRPGSRVLLLLLLLLLVYLTQPGNGNEGSVTGSCYCGKRISSDSPPSVQFMNRLRKHLRAYHRCLYYTRFQLLSWSVCGGNKDPWVQELMSCLDLKECGHAYSGIVAHQKHLLPTSPPISQASEGASSDIHTPAQMLLSTLQSTQRPTLPVGSLSSDKELTRPNETTIHTAGHSLAAGPEAGENQKQPEKNAGPTARTSATVPVLCLLAIIFILTAALSYVLCKRRRGQSPQSSPDLPVHYIPVAPDSNT

翻译修饰 - Q9H2A7 作为底物

Site PTM Type Enzyme
S45 Phosphorylation
S46 Phosphorylation
S48 Phosphorylation
S95 Phosphorylation

研究背景

功能:

Acts as a scavenger receptor on macrophages, which specifically binds to OxLDL (oxidized low density lipoprotein), suggesting that it may be involved in pathophysiology such as atherogenesis (By similarity). Induces a strong chemotactic response. Induces calcium mobilization. Binds to CXCR6/Bonzo.

翻译修饰:

Glycosylated.

细胞定位:

Cell membrane>Single-pass type I membrane protein. Secreted.
Note: Also exists as a soluble form.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in T-cell areas. Expressed in spleen, lymph nodes, lung, kidney, small intestine and thymus. Weak expression in heart and liver and no expression in brain and bone marrow.

蛋白家族:

Belongs to the intercrine alpha (chemokine CxC) family.

研究领域

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

文献引用

1). Traditional Chinese Medicine Shi-Bi-Man ameliorates psoriasis via inhibiting IL-23/Th17 axis and CXCL16-mediated endothelial activation. Chinese medicine, 2024 (PubMed: 38429819) [IF=4.9]

Application: IF/ICC    Species: Human    Sample: T cells

Fig. 7 SBM inhibits endothelial cells inflammatory response through Cxcl16. A Heatmap for CXCL signaling network. B Dotplot for the expression of genes related to CXCL signaling in endothelial cells and keratinocytes. C Immunofluorescence images staining for CD31 (green), CXCL16 (red), and DAPI (blue) of skin tissue, scale bar = 20 μm
2). A Mouse Model of Damp-Heat Syndrome in Traditional Chinese Medicine and Its Impact on Pancreatic Tumor Growth. Frontiers in Oncology, 2022 (PubMed: 35957897) [IF=4.7]

Application: WB    Species: Mice    Sample: tumor tissues

Figure 6 Chemokine differences between tumor-bearing mice with and without damp-heat syndrome. (A) Heatmap indicating serum chemokine distribution between the two groups (n = 10 in the T group, n = 9 in the TD group). (B) Heatmap indicating tumor tissue chemokine distribution between the two groups (n = 3 in each group). (C) Relative mRNA expression of tissue chemokines tested by qPCR (n = 10 in the T group, n = 9 in the TD group). (D) Relative protein expression of tissue chemokines tested by western blotting (n = 8 in each group). *p < 0.05, **p < 0.01, ***p < 0.001 compared with the T group.
3). Ginsenoside Rg1 Improves Inflammation and Autophagy of the Pancreas and Spleen in Streptozotocin-Induced Type 1 Diabetic Mice. International Journal of Endocrinology, 2023 (PubMed: 36960388) [IF=2.8]

Application: IF/ICC    Species: Mouse    Sample:

Figure 3 Expressions of CXCL16, CD45, and insulin receptor (INSR) proteins in the pancreas by immunofluorescence (×400, scale: 50 μm, mean ± SD, n = 6) (a–c) the expressions of CXCL16 and CD45 proteins' colocalization in the pancreas. (d–f) The expressions of INSR and CXCL16 proteins colocalization in the pancreas. The fluorescence intensity was detected to observe the protein expression. The increase in CD45 indicated an increase in inflammatory cell infiltration. Those results suggested that Rg1 treatment improves inflammation of the pancreas in type 1 diabetes mice relating to reducing CXCL16. ∗∗p < 0.01, vs. the control group; ##p < 0.01 vs. the DM group.

Application: WB    Species: Mouse    Sample:

Figure 8 The expression of CXCL16, NF-κB, TF, LC3, and P62 proteins in the pancreas and spleens. (Mean ± SD, n = 3) (a–f) the expression of CXCL16, NF-κB, TF, LC3, and P62 proteins in the pancreas. (g–l) The expression of CXCL16, NF-κB, TF, LC3, and P62 proteins in the spleens. The improvement of Rg1 treatment on type 1 diabetes mice was related to the increase of CXCL16, NF-κB, and TF and the raised autophagy (IL3 II/LC 3 I). ∗∗p < 0.01, vs. the control group; ns p > 0.05, ##p < 0.01 vs. the DM group.
4). YAP1/MMP7/CXCL16 axis affects efficacy of neoadjuvant chemotherapy via tumor environment immunosuppression in triple-negative breast cancer. Gland Surgery, 2021 (PubMed: 34733729) [IF=1.8]

Application: WB    Species: Human    Sample: HCC70 cell

Figure 4 Western blotting assay was employed to measure cytoplasmic YAP1 as well as soluble MMP7 and CXCL16 in extracellular matrix in MDA-MB-231 and HCC70 cell lines, respectively. Modulation sequence of protein expression was estimated via YAP1 inhibitor (verteporfin) and MMPs inhibitor (GM6001). Results were statistically analyzed

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