SNAP23 Antibody - #DF13314

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产品: SNAP23 抗体
货号: DF13314
描述: Rabbit polyclonal antibody to SNAP23
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 23kDa, (isoform)19 kDa; 23kD(Calculated).
蛋白号: O00161
RRID: AB_2846333

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   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
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实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(83%)
克隆:
Polyclonal
特异性:
SNAP23 Antibody detects endogenous levels of total SNAP23.
RRID:
AB_2846333
引用格式: Affinity Biosciences Cat# DF13314, RRID:AB_2846333.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

HsT17016; LS-B8340; SNAP 23; SNAP-23; SNAP23; SNAP23A; SNAP23B; SNP23_HUMAN; Synaptosomal associated protein 23; Synaptosomal associated protein 23kDa; Synaptosomal associated protein; Synaptosomal-associated protein 23; Vesicle membrane fusion protein SNAP 23; Vesicle membrane fusion protein SNAP23; Vesicle-membrane fusion protein SNAP-23;

抗原和靶标

免疫原:
Uniprot:

O00161(SNP23_HUMAN) >>Visit HPA database.

基因/基因ID:
SNAP23(8773)
表达:
O00161 SNP23_HUMAN:

Ubiquitous. Highest levels where found in placenta.

序列:
MDNLSSEEIQQRAHQITDESLESTRRILGLAIESQDAGIKTITMLDEQKEQLNRIEEGLDQINKDMRETEKTLTELNKCCGLCVCPCNRTKNFESGKAYKTTWGDGGENSPCNVVSKQPGPVTNGQLQQPTTGAASGGYIKRITNDAREDEMEENLTQVGSILGNLKDMALNIGNEIDAQNPQIKRITDKADTNRDRIDIANARAKKLIDS

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
83
Xenopus
57
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - O00161 作为底物

Site PTM Type Enzyme
M1 Acetylation
S5 Phosphorylation
S6 Phosphorylation
T17 Phosphorylation
S20 Phosphorylation
S23 Phosphorylation P17252 (PRKCA)
T24 Phosphorylation P17252 (PRKCA)
S34 Phosphorylation
K40 Ubiquitination
K49 Ubiquitination
K64 Ubiquitination
K71 Ubiquitination
T72 Phosphorylation
K91 Ubiquitination
S95 Phosphorylation P14618 (PKM)
K97 Ubiquitination
Y99 Phosphorylation
K100 Ubiquitination
T102 Phosphorylation
S110 Phosphorylation
C112 S-Nitrosylation
K117 Ubiquitination
S136 Phosphorylation
Y139 Phosphorylation
K141 Ubiquitination
T144 Phosphorylation
T157 Phosphorylation
S161 Phosphorylation P17252 (PRKCA)
K185 Ubiquitination
K190 Ubiquitination

研究背景

功能:

Essential component of the high affinity receptor for the general membrane fusion machinery and an important regulator of transport vesicle docking and fusion.

细胞定位:

Cell membrane>Peripheral membrane protein. Cell membrane>Lipid-anchor. Cell junction>Synapse>Synaptosome.
Note: Mainly localized to the plasma membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Ubiquitous. Highest levels where found in placenta.

亚基结构:

Homotetramer (via coiled-coil domain), also forms heterotetramers with STX4 and VAMP3. Found in a complex with VAMP8 and STX1A. Found in a complex with VAMP8 and STX4 in pancreas (By similarity). Interacts simultaneously with SNAPIN and SYN4 (By similarity). Interacts with STX1A (By similarity). Interacts with STX12 (By similarity). Interacts tightly to multiple syntaxins and synaptobrevins/VAMPs (By similarity). Interacts with ZDHHC13 (via ANK repeats) (By similarity). Interacts with ZDHHC17 (via ANK repeats).

蛋白家族:

Belongs to the SNAP-25 family.

研究领域

· Genetic Information Processing > Folding, sorting and degradation > SNARE interactions in vesicular transport.

· Organismal Systems > Immune system > Platelet activation.   (View pathway)

文献引用

1). Obstruction of the Tear Drainage Altered Lacrimal Gland Structure and Function. Investigative Ophthalmology & Visual Science, 2023 (PubMed: 37440262) [IF=4.4]

Application: IF/ICC    Species: Mouse    Sample:

Figure 7. Altered expression and localization of proteins relevant to lacrimal gland fusion and secretion. (A) Rab3d immunofluorescence was abundant in the apical lumen side of the acinar cells in the sham LGs (left panels), but uniformly distributed in the STDOB (middle panels) and more basally localized in the Prickle1 mutant acini. The bottom panels are magnified images from the top panels. Apical and basal positions of the acinus lumen are illustrated in the right corner of the first panel. (B) Western blotting demonstrated reduced Rab3d expression in the STDOB and Prickle1 mutant LGs compared with the sham. (C) Vamp8 immunofluorescence showed a similar altered pattern as the Rab3d in the STDOB and Prickle1 mice. (D) Western blotting showed increased LG expression of Vamp8 in the STDOB and Prickle1 mice. (E) Expression of Snap23 in LG acini was also basally shifted and showed increased levels in STDOB and Prickle1 mice compared with the sham (F). The white dotted lines indicate acini boundaries. For Western blotting and quantification, n = 4 LGs/mice.

Application: WB    Species: Mouse    Sample:

Figure 7. Altered expression and localization of proteins relevant to lacrimal gland fusion and secretion. (A) Rab3d immunofluorescence was abundant in the apical lumen side of the acinar cells in the sham LGs (left panels), but uniformly distributed in the STDOB (middle panels) and more basally localized in the Prickle1 mutant acini. The bottom panels are magnified images from the top panels. Apical and basal positions of the acinus lumen are illustrated in the right corner of the first panel. (B) Western blotting demonstrated reduced Rab3d expression in the STDOB and Prickle1 mutant LGs compared with the sham. (C) Vamp8 immunofluorescence showed a similar altered pattern as the Rab3d in the STDOB and Prickle1 mice. (D) Western blotting showed increased LG expression of Vamp8 in the STDOB and Prickle1 mice. (E) Expression of Snap23 in LG acini was also basally shifted and showed increased levels in STDOB and Prickle1 mice compared with the sham (F). The white dotted lines indicate acini boundaries. For Western blotting and quantification, n = 4 LGs/mice.

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