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Western blot analysis of extracts from Rat heart, using TFII I Antibody.

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DF13330 at 1/100 staining Human prostate cancer by IHC-P.

产品:	TFII I 抗体
货号:	DF13330
描述:	Rabbit polyclonal antibody to TFII I
应用:	WB IHC
反应:	Human, Mouse, Rat
预测:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量:	112kDa; 112kD(Calculated).
蛋白号:	P78347
RRID:	AB_2846349

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阻断肽(封闭肽)是特异性结合目标抗体和阻断抗体结合的多肽。这些多肽包含抗体识别的抗原表位。与阻断肽结合的抗体不再与靶蛋白上的表位结合。例如，在免疫印迹(WB)和免疫组化(IHC)中，通过比较被阻断抗体和未阻断抗体的染色，可以看到哪种染色是特异性的。

规格	价格	库存
100ul	RMB¥ 2300	现货
200ul	RMB¥ 3000	现货

货期: 当天发货

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实验方案

产品描述

来源:

Rabbit

应用:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:

Human,Mouse,Rat

预测:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)

克隆:

Polyclonal

特异性:

TFII I Antibody detects endogenous levels of total TFII I.

RRID:

AB_2846349
引用格式: Affinity Biosciences Cat# DF13330, RRID:AB_2846349.

偶联:

Unconjugated.

纯化:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

别名:

展开/折叠

BAP 135; BAP-135; BAP135; Bruton tyrosine kinase associated protein 135; Bruton tyrosine kinase-associated protein 135; BTK associated protein 135; BTK associated protein 135kD; BTK associated protein; BTK-associated protein 135; BTKAP 1; BTKAP1; DIWS; FLJ38776; FLJ56355; General transcription factor II i; General transcription factor II-I; General transcription factor IIi; GTF 2I; Gtf2i; GTF2I_HUMAN; GTFII I; GTFII-I; IB 291; IB291; SPIN; SRF Phox 1 interacting protein; SRF Phox1 interacting protein; SRF-Phox1-interacting protein; TFII-I; Transcription factor II I; WBS; WBSCR 6; WBSCR6; Williams Beuren syndrome chromosome region 6; Williams Beuren syndrome chromosome region 6 protein; Williams-Beuren syndrome chromosomal region 6 protein;

抗原和靶标

免疫原:

Uniprot:

P78347(GTF2I_HUMAN) >>Visit HPA database.

基因/基因ID:

GTF2I(2969)

表达:

P78347 GTF2I_HUMAN:

Ubiquitous. Isoform 1 is strongly expressed in fetal brain, weakly in adult brain, muscle, and lymphoblasts and is almost undetectable in other adult tissues, while the other isoforms are equally expressed in all adult tissues.

序列:

P78347 GTF2I_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAQVAMSTLPVEDEESSESRMVVTFLMSALESMCKELAKSKAEVACIAVYETDVFVVGTERGRAFVNTRKDFQKDFVKYCVEEEEKAAEMHKMKSTTQANRMSVDAVEIETLRKTVEDYFCFCYGKALGKSTVVPVPYEKMLRDQSAVVVQGLPEGVAFKHPENYDLATLKWILENKAGISFIIKRPFLEPKKHVGGRVMVTDADRSILSPGGSCGPIKVKTEPTEDSGISLEMAAVTVKEESEDPDYYQYNIQAGPSETDDVDEKQPLSKPLQGSHHSSEGNEGTEMEVPAEDSTQHVPSETSEDPEVEVTIEDDDYSPPSKRPKANELPQPPVPEPANAGKRKVREFNFEKWNARITDLRKQVEELFERKYAQAIKAKGPVTIPYPLFQSHVEDLYVEGLPEGIPFRRPSTYGIPRLERILLAKERIRFVIKKHELLNSTREDLQLDKPASGVKEEWYARITKLRKMVDQLFCKKFAEALGSTEAKAVPYQKFEAHPNDLYVEGLPENIPFRSPSWYGIPRLEKIIQVGNRIKFVIKRPELLTHSTTEVTQPRTNTPVKEDWNVRITKLRKQVEEIFNLKFAQALGLTEAVKVPYPVFESNPEFLYVEGLPEGIPFRSPTWFGIPRLERIVRGSNKIKFVVKKPELVISYLPPGMASKINTKALQSPKRPRSPGSNSKVPEIEVTVEGPNNNNPQTSAVRTPTQTNGSNVPFKPRGREFSFEAWNAKITDLKQKVENLFNEKCGEALGLKQAVKVPFALFESFPEDFYVEGLPEGVPFRRPSTFGIPRLEKILRNKAKIKFIIKKPEMFETAIKESTSSKSPPRKINSSPNVNTTASGVEDLNIIQVTIPDDDNERLSKVEKARQLREQVNDLFSRKFGEAIGMGFPVKVPYRKITINPGCVVVDGMPPGVSFKAPSYLEISSMRRILDSAEFIKFTVIRPFPGLVINNQLVDQSESEGPVIQESAEPSQLEVPATEEIKETDGSSQIKQEPDPTW

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P78347 作为底物

P78347

Site	PTM Type	Enzyme	Source
A2	Acetylation		Uniprot
T8	Phosphorylation		Uniprot
S16	Phosphorylation		Uniprot
S19	Phosphorylation		Uniprot
K74	Ubiquitination		Uniprot
K78	Ubiquitination		Uniprot
K86	Sumoylation		Uniprot
K94	Sumoylation		Uniprot
S95	Phosphorylation		Uniprot
R101	Methylation		Uniprot
S103	Phosphorylation		Uniprot
T111	Phosphorylation		Uniprot
C121	S-Nitrosylation		Uniprot
C123	S-Nitrosylation		Uniprot
K130	Sumoylation		Uniprot
K130	Ubiquitination		Uniprot
K140	Sumoylation		Uniprot
K140	Ubiquitination		Uniprot
R143	Methylation		Uniprot
S146	Phosphorylation		Uniprot
K160	Ubiquitination		Uniprot
K171	Ubiquitination		Uniprot
K177	Sumoylation		Uniprot
S181	Phosphorylation		Uniprot
T202	Phosphorylation		Uniprot
R206	Methylation		Uniprot
S207	Phosphorylation		Uniprot
S210	Phosphorylation		Uniprot
S214	Phosphorylation		Uniprot
K219	Methylation		Uniprot
K219	Ubiquitination		Uniprot
K221	Sumoylation		Uniprot
T238	Phosphorylation		Uniprot
K240	Sumoylation		Uniprot
Y248	Phosphorylation	P12931 (SRC) , Q06187 (BTK) , O60674 (JAK2)	Uniprot
Y249	Phosphorylation	P12931 (SRC)	Uniprot
Y318	Phosphorylation		Uniprot
S319	Phosphorylation		Uniprot
K326	Sumoylation		Uniprot
K343	Acetylation		Uniprot
K343	Methylation		Uniprot
K343	Sumoylation		Uniprot
K353	Sumoylation		Uniprot
K353	Ubiquitination		Uniprot
K363	Sumoylation		Uniprot
K363	Ubiquitination		Uniprot
K372	Sumoylation		Uniprot
K372	Ubiquitination		Uniprot
K378	Sumoylation		Uniprot
K378	Ubiquitination		Uniprot
K380	Ubiquitination		Uniprot
Y398	Phosphorylation	Q06187 (BTK)	Uniprot
S412	Phosphorylation	Q13976 (PRKG1)	Uniprot
T413	Phosphorylation		Uniprot
K435	Sumoylation		Uniprot
K435	Ubiquitination		Uniprot
K450	Acetylation		Uniprot
K450	Sumoylation		Uniprot
K450	Ubiquitination		Uniprot
S453	Phosphorylation		Uniprot
K456	Methylation		Uniprot
K456	Sumoylation		Uniprot
K456	Ubiquitination		Uniprot
Y460	Phosphorylation		Uniprot
C475	S-Nitrosylation		Uniprot
K476	Ubiquitination		Uniprot
K477	Sumoylation		Uniprot
K477	Ubiquitination		Uniprot
K488	Sumoylation		Uniprot
K488	Ubiquitination		Uniprot
K494	Sumoylation		Uniprot
K494	Ubiquitination		Uniprot
Y503	Phosphorylation	Q06187 (BTK)	Uniprot
S515	Phosphorylation		Uniprot
S517	Phosphorylation		Uniprot
Y519	Phosphorylation		Uniprot
K526	Sumoylation		Uniprot
K526	Ubiquitination		Uniprot
K539	Ubiquitination		Uniprot
S547	Phosphorylation		Uniprot
T556	Phosphorylation	P24941 (CDK2)	Uniprot
T558	Phosphorylation	P24941 (CDK2)	Uniprot
K561	Acetylation		Uniprot
K561	Sumoylation		Uniprot
K561	Ubiquitination		Uniprot
K573	Ubiquitination		Uniprot
S620	Phosphorylation		Uniprot
K640	Sumoylation		Uniprot
K645	Sumoylation		Uniprot
S659	Phosphorylation		Uniprot
K660	Ubiquitination		Uniprot
T663	Phosphorylation		Uniprot
K664	Sumoylation		Uniprot
K664	Ubiquitination		Uniprot
S668	Phosphorylation	P28482 (MAPK1) , P27361 (MAPK3)	Uniprot
S674	Phosphorylation	P27361 (MAPK3) , P28482 (MAPK1)	Uniprot
S677	Phosphorylation		Uniprot
S679	Phosphorylation		Uniprot
T687	Phosphorylation		Uniprot
T698	Phosphorylation		Uniprot
S699	Phosphorylation		Uniprot
T703	Phosphorylation		Uniprot
T705	Phosphorylation		Uniprot
K715	Acetylation		Uniprot
K715	Methylation		Uniprot
K715	Sumoylation		Uniprot
K715	Ubiquitination		Uniprot
S722	Phosphorylation		Uniprot
K729	Ubiquitination		Uniprot
K734	Sumoylation		Uniprot
K736	Sumoylation		Uniprot
K736	Ubiquitination		Uniprot
K744	Sumoylation		Uniprot
K744	Ubiquitination		Uniprot
K752	Sumoylation		Uniprot
K752	Ubiquitination		Uniprot
K756	Ubiquitination		Uniprot
Y770	Phosphorylation		Uniprot
S784	Phosphorylation	Q13976 (PRKG1)	Uniprot
T785	Phosphorylation		Uniprot
K806	Ubiquitination		Uniprot
K807	Sumoylation		Uniprot
K807	Ubiquitination		Uniprot
K816	Sumoylation		Uniprot
S818	Phosphorylation		Uniprot
S820	Phosphorylation		Uniprot
S821	Phosphorylation		Uniprot
S823	Phosphorylation		Uniprot
K827	Ubiquitination		Uniprot
S830	Phosphorylation		Uniprot
S831	Phosphorylation		Uniprot
T836	Phosphorylation		Uniprot
T837	Phosphorylation		Uniprot
S839	Phosphorylation		Uniprot
T850	Phosphorylation		Uniprot
K861	Sumoylation		Uniprot
S877	Phosphorylation		Uniprot
K879	Sumoylation		Uniprot
K879	Ubiquitination		Uniprot
K891	Sumoylation		Uniprot
K891	Ubiquitination		Uniprot
Y894	Phosphorylation		Uniprot
K896	Sumoylation		Uniprot
K896	Ubiquitination		Uniprot
Y920	Phosphorylation		Uniprot
S925	Phosphorylation		Uniprot
R927	Methylation		Uniprot
S932	Phosphorylation		Uniprot
S971	Phosphorylation		Uniprot
K982	Sumoylation		Uniprot
S988	Phosphorylation		Uniprot
K991	Sumoylation		Uniprot
K991	Ubiquitination		Uniprot

研究背景

P78347_GTF2I_HUMAN

功能:

Interacts with the basal transcription machinery by coordinating the formation of a multiprotein complex at the C-FOS promoter, and linking specific signal responsive activator complexes. Promotes the formation of stable high-order complexes of SRF and PHOX1 and interacts cooperatively with PHOX1 to promote serum-inducible transcription of a reporter gene deriven by the C-FOS serum response element (SRE). Acts as a coregulator for USF1 by binding independently two promoter elements, a pyrimidine-rich initiator (Inr) and an upstream E-box. Required for the formation of functional ARID3A DNA-binding complexes and for activation of immunoglobulin heavy-chain transcription upon B-lymphocyte activation.

翻译修饰:

Transiently phosphorylated on tyrosine residues by BTK in response to B-cell receptor stimulation. Phosphorylation on Tyr-248 and Tyr-398, and perhaps, on Tyr-503 contributes to BTK-mediated transcriptional activation.

Sumoylated.

细胞定位:

Cytoplasm. Nucleus.
Note: Colocalizes with BTK in the cytoplasm.

组织特异性:

亚基结构:

Homodimer (Potential). Interacts with SRF and PHOX1. Binds a pyrimidine-rich initiator (Inr) and a recognition site (E-box) for upstream stimulatory factor 1 (USF1). Associates with the PH domain of Bruton's tyrosine kinase (BTK). May be a component of a BHC histone deacetylase complex that contains HDAC1, HDAC2, HMG20B/BRAF35, KDM1A, RCOR1/CoREST, PHF21A/BHC80, ZMYM2, ZNF217, ZMYM3, GSE1 and GTF2I. Interacts with BTK and ARID3A. Interacts with isoform beta of PRKG1.

蛋白家族:

Belongs to the TFII-I family.

研究领域

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway. (View pathway)

· Genetic Information Processing > Transcription > Basal transcription factors.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

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TFII I Antibody - #DF13330

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