产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF13333, RRID:AB_2846352.
展开/折叠
DKFZp686O09161; MCPH7; OTTHUMP00000009566; SCL interrupting locus protein; SCL-interrupting locus protein; SCL/TAL1 interrupting locus; SIL; STIL; STIL_HUMAN; TAL 1 interrupting locus protein; TAL-1-interrupting locus protein;
抗原和靶标
Expressed in all hematopoietic tissues and cell lines. Highly expressed in a variety of tumors characterized by increased mitotic activity with highest expression in lung cancer.
- Q15468 STIL_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEPIYPFARPQMNTRFPSSRMVPFHFPPSKCALWNPTPTGDFIYLHLSYYRNPKLVVTEKTIRLAYRHAKQNKKNSSCFLLGSLTADEDEEGVTLTVDRFDPGREVPECLEITPTASLPGDFLIPCKVHTQELCSREMIVHSVDDFSSALKALQCHICSKDSLDCGKLLSLRVHITSRESLDSVEFDLHWAAVTLANNFKCTPVKPIPIIPTALARNLSSNLNISQVQGTYKYGYLTMDETRKLLLLLESDPKVYSLPLVGIWLSGITHIYSPQVWACCLRYIFNSSVQERVFSESGNFIIVLYSMTHKEPEFYECFPCDGKIPDFRFQLLTSKETLHLFKNVEPPDKNPIRCELSAESQNAETEFFSKASKNFSIKRSSQKLSSGKMPIHDHDSGVEDEDFSPRPIPSPHPVSQKISKIQPSVPELSLVLDGNFIESNPLPTPLEMVNNENPPLINHLEHLKPLQPQLYDEKHSPEVEAGEPSLRGIPNQLNQDKPALLRHCKVRQPPAYKKGNPHTRNSIKPSSHNGPSHDIFEKLQTVSAGNVQNEEYPIRPSTLNSRQSSLAPQSQPHDFVFSPHNSGRPMELQIPTPPLPSYCSTNVCRCCQHHSHIQYSPLNSWQGANTVGSIQDVQSEALQKHSLFHPSGCPALYCNAFCSSSSPIALRPQGDMGSCSPHSNIEPSPVARPPSHMDLCNPQPCTVCMHTPKTESDNGMMGLSPDAYRFLTEQDRQLRLLQAQIQRLLEAQSLMPCSPKTTAVEDTVQAGRQMELVSVEAQSSPGLHMRKGVSIAVSTGASLFWNAAGEDQEPDSQMKQDDTKISSEDMNFSVDINNEVTSLPGSASSLKAVDIPSFEESNIAVEEEFNQPLSVSNSSLVVRKEPDVPVFFPSGQLAESVSMCLQTGPTGGASNNSETSEEPKIEHVMQPLLHQPSDNQKIYQDLLGQVNHLLNSSSKETEQPSTKAVIISHECTRTQNVYHTKKKTHHSRLVDKDCVLNATLKQLRSLGVKIDSPTKVKKNAHNVDHASVLACISPEAVISGLNCMSFANVGMSGLSPNGVDLSMEANAIALKYLNENQLSQLSVTRSNQNNCDPFSLLHINTDRSTVGLSLISPNNMSFATKKYMKRYGLLQSSDNSEDEEEPPDNADSKSEYLLNQNLRSIPEQLGGQKEPSKNDHEIINCSNCESVGTNADTPVLRNITNEVLQTKAKQQLTEKPAFLVKNLKPSPAVNLRTGKAEFTQHPEKENEGDITIFPESLQPSETLKQMNSMNSVGTFLDVKRLRQLPKLF
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q15468 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| K54 | Ubiquitination | Uniprot | |
| K60 | Ubiquitination | Uniprot | |
| T94 | Phosphorylation | Uniprot | |
| T96 | Phosphorylation | Uniprot | |
| K127 | Ubiquitination | Uniprot | |
| S147 | Phosphorylation | Uniprot | |
| S148 | Phosphorylation | Uniprot | |
| K151 | Ubiquitination | Uniprot | |
| S162 | Phosphorylation | Uniprot | |
| K243 | Ubiquitination | Uniprot | |
| S272 | Phosphorylation | Uniprot | |
| K334 | Ubiquitination | Uniprot | |
| T336 | Phosphorylation | Uniprot | |
| K341 | Ubiquitination | Uniprot | |
| K348 | Ubiquitination | Uniprot | |
| K369 | Ubiquitination | Uniprot | |
| K372 | Ubiquitination | Uniprot | |
| S375 | Phosphorylation | Uniprot | |
| S395 | Phosphorylation | Uniprot | |
| S403 | Phosphorylation | Uniprot | |
| S409 | Phosphorylation | Uniprot | |
| K416 | Ubiquitination | Uniprot | |
| Y470 | Phosphorylation | Uniprot | |
| S475 | Phosphorylation | Uniprot | |
| K496 | Ubiquitination | Uniprot | |
| K512 | Acetylation | Uniprot | |
| S521 | Phosphorylation | Uniprot | |
| K523 | Ubiquitination | Uniprot | |
| T591 | Phosphorylation | Uniprot | |
| S719 | Phosphorylation | Uniprot | |
| S753 | Phosphorylation | Uniprot | |
| K755 | Ubiquitination | Uniprot | |
| S779 | Phosphorylation | Uniprot | |
| S852 | Phosphorylation | Uniprot | |
| S869 | Phosphorylation | Uniprot | |
| S871 | Phosphorylation | Uniprot | |
| S873 | Phosphorylation | Uniprot | |
| S874 | Phosphorylation | Uniprot | |
| K936 | Ubiquitination | Uniprot | |
| Y938 | Phosphorylation | Uniprot | |
| K954 | Ubiquitination | Uniprot | |
| K962 | Ubiquitination | Uniprot | |
| K991 | Ubiquitination | Uniprot | |
| K1000 | Ubiquitination | Uniprot | |
| S1004 | Phosphorylation | Uniprot | |
| K1008 | Ubiquitination | Uniprot | |
| S1011 | Phosphorylation | Uniprot | |
| K1014 | Ubiquitination | Uniprot | |
| S1061 | Phosphorylation | Uniprot | |
| S1108 | Phosphorylation | P53350 (PLK1) | Uniprot |
| S1111 | Phosphorylation | Uniprot | |
| S1116 | Phosphorylation | P53350 (PLK1) | Uniprot |
| T1119 | Phosphorylation | Uniprot | |
| K1120 | Ubiquitination | Uniprot | |
| S1132 | Phosphorylation | Uniprot | |
| S1135 | Phosphorylation | Uniprot | |
| K1168 | Ubiquitination | Uniprot | |
| K1206 | Ubiquitination | Uniprot | |
| K1214 | Ubiquitination | Uniprot | |
| K1223 | Ubiquitination | Uniprot | |
| S1225 | Phosphorylation | Uniprot | |
| K1234 | Ubiquitination | Uniprot | |
| K1243 | Ubiquitination | Uniprot | |
| T1250 | Phosphorylation | Uniprot |
研究背景
Immediate-early gene. Plays an important role in embryonic development as well as in cellular growth and proliferation; its long-term silencing affects cell survival and cell cycle distribution as well as decreases CDK1 activity correlated with reduced phosphorylation of CDK1. Plays a role as a positive regulator of the sonic hedgehog pathway, acting downstream of PTCH1. Plays an important role in the regulation of centriole duplication. Required for the onset of procentriole formation and proper mitotic progression. During procentriole formation, is essential for the correct loading of SASS6 and CENPJ to the base of the procentriole to initiate procentriole assembly.
Ubiquitinated.
Phosphorylated following the activation of the mitotic checkpoint.
Cytoplasm>Cytosol. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome>Centriole.
Expressed in all hematopoietic tissues and cell lines. Highly expressed in a variety of tumors characterized by increased mitotic activity with highest expression in lung cancer.
Homodimer. Interacts with PIN1 via its WW domain. This interaction is dependent on STIL mitotic phosphorylation (By similarity). Interacts with CENPJ. Interacts with RBM14 and this interaction interferes with the interaction of STIL with CENPJ. Forms a complex with CENPJ and SASS6.
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