(1)
(3)
| 产品: | FOXA2 抗体 |
| 货号: | DF13363 |
| 描述: | Rabbit polyclonal antibody to FOXA2 |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| 分子量: | 48kDa; 48kD(Calculated). |
| 蛋白号: | Q9Y261 |
| RRID: | AB_2846382 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF13363, RRID:AB_2846382.
展开/折叠
Forkhead box A2; Forkhead box protein A2; FOX A2; foxa2; FOXA2_HUMAN; Hepatic nuclear factor 3 beta; Hepatocyte nuclear factor 3; Hepatocyte nuclear factor 3 beta; Hepatocyte nuclear factor 3-beta; HNF 3B; HNF-3-beta; HNF-3B; HNF3B; MGC19807; TCF 3B; TCF-3B; TCF3B; Transcription factor 3B;
抗原和靶标
- Q9Y261 FOXA2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MLGAVKMEGHEPSDWSSYYAEPEGYSSVSNMNAGLGMNGMNTYMSMSAAAMGSGSGNMSAGSMNMSSYVGAGMSPSLAGMSPGAGAMAGMGGSAGAAGVAGMGPHLSPSLSPLGGQAAGAMGGLAPYANMNSMSPMYGQAGLSRARDPKTYRRSYTHAKPPYSYISLITMAIQQSPNKMLTLSEIYQWIMDLFPFYRQNQQRWQNSIRHSLSFNDCFLKVPRSPDKPGKGSFWTLHPDSGNMFENGCYLRRQKRFKCEKQLALKEAAGAAGSGKKAAAGAQASQAQLGEAAGPASETPAGTESPHSSASPCQEHKRGGLGELKGTPAAALSPPEPAPSPGQQQQAAAHLLGPPHHPGLPPEAHLKPEHHYAFNHPFSINNLMSSEQQHHHSHHHHQPHKMDLKAYEQVMHYPGYGSPMPGSLAMGPVTNKTGLDASPLAADTSYYQGVYSRPIMNSS
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9Y261 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K6 | Acetylation | Uniprot | |
| S107 | Phosphorylation | O15111 (CHUK) | Uniprot |
| S111 | Phosphorylation | O15111 (CHUK) | Uniprot |
| T156 | Phosphorylation | P31749 (AKT1) | Uniprot |
| S212 | Phosphorylation | Uniprot | |
| S223 | Phosphorylation | Uniprot | |
| Y248 | Phosphorylation | Uniprot | |
| K256 | Acetylation | Uniprot | |
| K259 | Acetylation | Uniprot | |
| K264 | Acetylation | Uniprot | |
| K274 | Acetylation | Uniprot | |
| K275 | Acetylation | Uniprot | |
| S283 | Phosphorylation | Uniprot | |
| S295 | Phosphorylation | Uniprot | |
| T301 | Phosphorylation | Uniprot | |
| S303 | Phosphorylation | Uniprot | |
| S307 | Phosphorylation | Uniprot | |
| S309 | Phosphorylation | Uniprot |
研究背景
Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). In embryonic development is required for notochord formation. Involved in the development of multiple endoderm-derived organ systems such as the liver, pancreas and lungs; FOXA1 and FOXA2 seem to have at least in part redundant roles. Originally described as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis; regulates the expression of genes important for glucose sensing in pancreatic beta-cells and glucose homeostasis. Involved in regulation of fat metabolism. Binds to fibrinogen beta promoter and is involved in IL6-induced fibrinogen beta transcriptional activation.
Phosphorylation on Thr-156 abolishes binding to target promoters and subsequent transcription activation upon insulin stimulation.
Nucleus. Cytoplasm.
Note: Shuttles between the nucleus and cytoplasm in a CRM1-dependent manner; in response to insulin signaling via AKT1 is exported from the nucleus.
Binds DNA as a monomer. Binds TLE1 (By similarity). Interacts with FOXA1 and FOXA3. Interacts with PRKDC.
研究领域
· Human Diseases > Endocrine and metabolic diseases > Maturity onset diabetes of the young.
· Organismal Systems > Aging > Longevity regulating pathway - multiple species. (View pathway)
文献引用
Application: WB Species: Mouse Sample:
限制条款
产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。
产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。
Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。产品仅供科学研究使用。不用于诊断和治疗。
产品未经授权不得转售。
Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.