| 产品: | EST1A 抗体 |
| 货号: | DF13372 |
| 描述: | Rabbit polyclonal antibody to EST1A |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus |
| 分子量: | 160kDa; 160kD(Calculated). |
| 蛋白号: | Q86US8 |
| RRID: | AB_2846391 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF13372, RRID:AB_2846391.
展开/折叠
C17orf31; EST1-like protein A; Ever shorter telomeres 1A; hSmg5/7a; KIAA0732; Smg-6 homolog; SMG6; Telomerase subunit EST1A; Telomerase-binding protein EST1A;
抗原和靶标
- Q86US8 EST1A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAEGLERVRISASELRGILATLAPQAGSRENMKELKEARPRKDNRRPDLEIYKPGLSRLRNKPKIKEPPGSEEFKDEIVNDRDCSAVENGTQPVKDVCKELNNQEQNGPIDPENNRGQESFPRTAGQEDRSLKIIKRTKKPDLQIYQPGRRLQTVSKESASRVEEEEVLNQVEQLRVEEDECRGNVAKEEVANKPDRAEIEKSPGGGRVGAAKGEKGKRMGKGEGVRETHDDPARGRPGSAKRYSRSDKRRNRYRTRSTSSAGSNNSAEGAGLTDNGCRRRRQDRTKERPRLKKQVSVSSTDSLDEDRIDEPDGLGPRRSSERKRHLERNWSGRGEGEQKNSAKEYRGTLRVTFDAEAMNKESPMVRSARDDMDRGKPDKGLSSGGKGSEKQESKNPKQELRGRGRGILILPAHTTLSVNSAGSPESAPLGPRLLFGSGSKGSRSWGRGGTTRRLWDPNNPDQKPALKTQTPQLHFLDTDDEVSPTSWGDSRQAQASYYKFQNSDNPYYYPRTPGPASQYPYTGYNPLQYPVGPTNGVYPGPYYPGYPTPSGQYVCSPLPTSTMSPEEVEQHMRNLQQQELHRLLRVADNQELQLSNLLSRDRISPEGLEKMAQLRAELLQLYERCILLDIEFSDNQNVDQILWKNAFYQVIEKFRQLVKDPNVENPEQIRNRLLELLDEGSDFFDSLLQKLQVTYKFKLEDYMDGLAIRSKPLRKTVKYALISAQRCMICQGDIARYREQASDTANYGKARSWYLKAQHIAPKNGRPYNQLALLAVYTRRKLDAVYYYMRSLAASNPILTAKESLMSLFEETKRKAEQMEKKQHEEFDLSPDQWRKGKKSTFRHVGDDTTRLEIWIHPSHPRSSQGTESGKDSEQENGLGSLSPSDLNKRFILSFLHAHGKLFTRIGMETFPAVAEKVLKEFQVLLQHSPSPIGSTRMLQLMTINMFAVHNSQLKDCFSEECRSVIQEQAAALGLAMFSLLVRRCTCLLKESAKAQLSSPEDQDDQDDIKVSSFVPDLKELLPSVKVWSDWMLGYPDTWNPPPTSLDLPSHVAVDVWSTLADFCNILTAVNQSEVPLYKDPDDDLTLLILEEDRLLSGFVPLLAAPQDPCYVEKTSDKVIAADCKRVTVLKYFLEALCGQEEPLLAFKGGKYVSVAPVPDTMGKEMGSQEGTRLEDEEEDVVIEDFEEDSEAEGSGGEDDIRELRAKKLALARKIAEQQRRQEKIQAVLEDHSQMRQMELEIRPLFLVPDTNGFIDHLASLARLLESRKYILVVPLIVINELDGLAKGQETDHRAGGYARVVQEKARKSIEFLEQRFESRDSCLRALTSRGNELESIAFRSEDITGQLGNNDDLILSCCLHYCKDKAKDFMPASKEEPIRLLREVVLLTDDRNLRVKALTRNVPVRDIPAFLTWAQVG
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q86US8 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S11 | Phosphorylation | Uniprot | |
| S28 | Phosphorylation | Uniprot | |
| Y52 | Phosphorylation | Uniprot | |
| S57 | Phosphorylation | Uniprot | |
| S85 | Phosphorylation | Uniprot | |
| T91 | Phosphorylation | Uniprot | |
| T138 | Phosphorylation | Uniprot | |
| Y146 | Phosphorylation | Uniprot | |
| S203 | Phosphorylation | Uniprot | |
| S240 | Phosphorylation | Uniprot | |
| S245 | Phosphorylation | Uniprot | |
| S247 | Phosphorylation | Uniprot | |
| T256 | Phosphorylation | Uniprot | |
| S260 | Phosphorylation | Uniprot | |
| S264 | Phosphorylation | Uniprot | |
| S267 | Phosphorylation | Uniprot | |
| S297 | Phosphorylation | Uniprot | |
| S332 | Phosphorylation | Uniprot | |
| T353 | Phosphorylation | Uniprot | |
| S363 | Phosphorylation | Uniprot | |
| R404 | Methylation | Uniprot | |
| R406 | Methylation | Uniprot | |
| S424 | Phosphorylation | Uniprot | |
| S438 | Phosphorylation | Uniprot | |
| S440 | Phosphorylation | Uniprot | |
| K441 | Ubiquitination | Uniprot | |
| S443 | Phosphorylation | Uniprot | |
| T471 | Phosphorylation | Uniprot | |
| T479 | Phosphorylation | Uniprot | |
| S484 | Phosphorylation | Uniprot | |
| T486 | Phosphorylation | Uniprot | |
| S487 | Phosphorylation | Uniprot | |
| Y508 | Phosphorylation | Uniprot | |
| Y509 | Phosphorylation | Uniprot | |
| R512 | Methylation | Uniprot | |
| Y769 | Phosphorylation | Uniprot | |
| Y778 | Phosphorylation | Uniprot | |
| Y788 | Phosphorylation | Uniprot | |
| Y789 | Phosphorylation | Uniprot | |
| S796 | Phosphorylation | Uniprot | |
| K803 | Acetylation | Uniprot | |
| K814 | Ubiquitination | Uniprot | |
| K823 | Ubiquitination | Uniprot | |
| S831 | Phosphorylation | Uniprot | |
| S864 | Phosphorylation | Uniprot | |
| S865 | Phosphorylation | Uniprot | |
| T868 | Phosphorylation | Uniprot | |
| S870 | Phosphorylation | Uniprot | |
| S874 | Phosphorylation | Uniprot | |
| S884 | Phosphorylation | Uniprot | |
| K918 | Acetylation | Uniprot | |
| K995 | Ubiquitination | Uniprot | |
| S1000 | Phosphorylation | Uniprot | |
| K1011 | Ubiquitination | Uniprot | |
| K1149 | Ubiquitination | Uniprot | |
| K1225 | Ubiquitination | Uniprot | |
| K1309 | Ubiquitination | Uniprot | |
| K1376 | Ubiquitination | Uniprot | |
| T1390 | Phosphorylation | Uniprot |
研究背景
Component of the telomerase ribonucleoprotein (RNP) complex that is essential for the replication of chromosome termini. May have a general role in telomere regulation. Promotes in vitro the ability of TERT to elongate telomeres. Overexpression induces telomere uncapping, chromosomal end-to-end fusions (telomeric DNA persists at the fusion points) and did not perturb TRF2 telomeric localization. Binds to the single-stranded 5'-(GTGTGG)(4)GTGT-3' telomeric DNA, but not to a telomerase RNA template component (TER).
Plays a role in nonsense-mediated mRNA decay. Is thought to provide a link to the mRNA degradation machinery as it has endonuclease activity required to initiate NMD, and to serve as an adapter for UPF1 to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation. Degrades single-stranded RNA (ssRNA), but not ssDNA or dsRNA.
Nucleus>Nucleolus. Chromosome>Telomere. Cytoplasm>Cytosol.
Note: Particularly enriched in the nucleolus.
Ubiquitous.
May form homooligomers. Associated component of the telomerase holoenzyme complex. Interacts with TERT, independently of the telomerase RNA. Interacts with SMG1, SMG5, SMG7, UPF1, UPF2, UPF3B and the PP2A catalytic subunits. Also interacts with the exon junction complex (EJC) composed at least of CASC3, EIF4A3, MAGOH and RBM8A; required for the process of nonsense-mediated mRNA decay.
The PINc domain confers endonuclease activity and is expected to bind the catalytic metal ion.
研究领域
· Genetic Information Processing > Translation > mRNA surveillance pathway.
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