产品: | hSET1 抗体 |
货号: | DF13382 |
描述: | Rabbit polyclonal antibody to hSET1 |
应用: | WB |
反应: | Human |
预测: | Pig, Bovine, Horse, Dog |
分子量: | 186kDa; 186kD(Calculated). |
蛋白号: | O15047 |
RRID: | AB_2846401 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF13382, RRID:AB_2846401.
展开/折叠
Histone-lysine N-methyltransferase SETD1A; hSET1A; KMT2F; Lysine N methyltransferase 2F; Lysine N-methyltransferase 2F; SET domain containing 1A; SET domain containing protein 1A; SET domain-containing protein 1A; SET1; Set1 Ash2 histone methyltransferase complex subunit SET1; Set1/Ash2 histone methyltransferase complex subunit SET1; SET1A; SET1A_HUMAN; SETD1A;
抗原和靶标
- O15047 SET1A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDQEGGGDGQKAPSFQWRNYKLIVDPALDPALRRPSQKVYRYDGVHFSVNDSKYIPVEDLQDPRCHVRSKNRDFSLPVPKFKLDEFYIGQIPLKEVTFARLNDNVRETFLKDMCRKYGEVEEVEILLHPRTRKHLGLARVLFTSTRGAKETVKNLHLTSVMGNIIHAQLDIKGQQRMKYYELIVNGSYTPQTVPTGGKALSEKFQGSGAATETAESRRRSSSDTAAYPAGTTAVGTPGNGTPCSQDTSFSSSRQDTPSSFGQFTPQSSQGTPYTSRGSTPYSQDSAYSSSTTSTSFKPRRSENSYQDAFSRRHFSASSASTTASTAIAATTAATASSSASSSSLSSSSSSSSSSSSSQFRSSDANYPAYYESWNRYQRHTSYPPRRATREEPPGAPFAENTAERFPPSYTSYLPPEPSRPTDQDYRPPASEAPPPEPPEPGGGGGGGGPSPEREEVRTSPRPASPARSGSPAPETTNESVPFAQHSSLDSRIEMLLKEQRSKFSFLASDTEEEEENSSMVLGARDTGSEVPSGSGHGPCTPPPAPANFEDVAPTGSGEPGATRESPKANGQNQASPCSSGDDMEISDDDRGGSPPPAPTPPQQPPPPPPPPPPPPPYLASLPLGYPPHQPAYLLPPRPDGPPPPEYPPPPPPPPHIYDFVNSLELMDRLGAQWGGMPMSFQMQTQMLTRLHQLRQGKGLIAASAGPPGGAFGEAFLPFPPPQEAAYGLPYALYAQGQEGRGAYSREAYHLPMPMAAEPLPSSSVSGEEARLPPREEAELAEGKTLPTAGTVGRVLAMLVQEMKSIMQRDLNRKMVENVAFGAFDQWWESKEEKAKPFQNAAKQQAKEEDKEKTKLKEPGLLSLVDWAKSGGTTGIEAFAFGSGLRGALRLPSFKVKRKEPSEISEASEEKRPRPSTPAEEDEDDPEQEKEAGEPGRPGTKPPKRDEERGKTQGKHRKSFALDSEGEEASQESSSEKDEEDDEEDEEDEDREEAVDTTKKETEVSDGEDEESDSSSKCSLYADSDGENDSTSDSESSSSSSSSSSSSSSSSSSSSSSSSESSSEDEEEEERPAALPSASPPPREVPVPTPAPVEVPVPERVAGSPVTPLPEQEASPARPAGPTEESPPSAPLRPPEPPAGPPAPAPRPDERPSSPIPLLPPPKKRRKTVSFSAIEVVPAPEPPPATPPQAKFPGPASRKAPRGVERTIRNLPLDHASLVKSWPEEVSRGGRSRAGGRGRLTEEEEAEPGTEVDLAVLADLALTPARRGLPALPAVEDSEATETSDEAERPRPLLSHILLEHNYALAVKPTPPAPALRPPEPVPAPAALFSSPADEVLEAPEVVVAEAEEPKPQQLQQQREEGEEEGEEEGEEEEEESSDSSSSSDGEGALRRRSLRSHARRRRPPPPPPPPPPRAYEPRSEFEQMTILYDIWNSGLDSEDMSYLRLTYERLLQQTSGADWLNDTHWVHHTITNLTTPKRKRRPQDGPREHQTGSARSEGYYPISKKEKDKYLDVCPVSARQLEGVDTQGTNRVLSERRSEQRRLLSAIGTSAIMDSDLLKLNQLKFRKKKLRFGRSRIHEWGLFAMEPIAADEMVIEYVGQNIRQMVADMREKRYVQEGIGSSYLFRVDHDTIIDATKCGNLARFINHCCTPNCYAKVITIESQKKIVIYSKQPIGVDEEITYDYKFPLEDNKIPCLCGTESCRGSLN
种属预测
score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O15047 作为底物
Site | PTM Type | Enzyme | Source |
---|---|---|---|
K21 | Ubiquitination | Uniprot | |
T97 | Phosphorylation | Uniprot | |
K111 | Ubiquitination | Uniprot | |
Y179 | Phosphorylation | Uniprot | |
Y180 | Phosphorylation | Uniprot | |
Y188 | Phosphorylation | Uniprot | |
S207 | Phosphorylation | Uniprot | |
S220 | Phosphorylation | Uniprot | |
S221 | Phosphorylation | Uniprot | |
S222 | Phosphorylation | Uniprot | |
T224 | Phosphorylation | Uniprot | |
S268 | Phosphorylation | Uniprot | |
T271 | Phosphorylation | Uniprot | |
Y273 | Phosphorylation | Uniprot | |
T274 | Phosphorylation | Uniprot | |
R276 | Methylation | Uniprot | |
S282 | Phosphorylation | Uniprot | |
S293 | Phosphorylation | Uniprot | |
S315 | Phosphorylation | Uniprot | |
T330 | Phosphorylation | Uniprot | |
S343 | Phosphorylation | Uniprot | |
S347 | Phosphorylation | Uniprot | |
S348 | Phosphorylation | Uniprot | |
S349 | Phosphorylation | Uniprot | |
S350 | Phosphorylation | Uniprot | |
S351 | Phosphorylation | Uniprot | |
S352 | Phosphorylation | Uniprot | |
Y366 | Phosphorylation | Uniprot | |
Y370 | Phosphorylation | Uniprot | |
T388 | Phosphorylation | Uniprot | |
Y409 | Phosphorylation | Uniprot | |
S450 | Phosphorylation | Uniprot | |
S459 | Phosphorylation | Uniprot | |
S464 | Phosphorylation | Uniprot | |
S468 | Phosphorylation | Uniprot | |
S470 | Phosphorylation | Uniprot | |
T476 | Phosphorylation | Uniprot | |
S501 | Phosphorylation | Uniprot | |
S504 | Phosphorylation | Uniprot | |
S508 | Phosphorylation | Uniprot | |
T510 | Phosphorylation | Uniprot | |
S534 | Phosphorylation | Uniprot | |
T540 | Phosphorylation | Uniprot | |
T562 | Phosphorylation | Uniprot | |
S565 | Phosphorylation | Uniprot | |
S586 | Phosphorylation | Uniprot | |
K803 | Acetylation | Uniprot | |
S882 | Phosphorylation | Uniprot | |
S892 | Phosphorylation | Uniprot | |
K898 | Acetylation | Uniprot | |
S904 | Phosphorylation | Uniprot | |
S907 | Phosphorylation | Uniprot | |
K910 | Acetylation | Uniprot | |
S915 | Phosphorylation | Uniprot | |
T916 | Phosphorylation | Uniprot | |
S958 | Phosphorylation | Uniprot | |
S963 | Phosphorylation | Uniprot | |
S969 | Phosphorylation | Uniprot | |
S972 | Phosphorylation | Uniprot | |
S973 | Phosphorylation | Uniprot | |
S1004 | Phosphorylation | Uniprot | |
S1037 | Phosphorylation | Uniprot | |
S1038 | Phosphorylation | Uniprot | |
S1039 | Phosphorylation | Uniprot | |
S1040 | Phosphorylation | Uniprot | |
S1041 | Phosphorylation | Uniprot | |
S1046 | Phosphorylation | Uniprot | |
S1047 | Phosphorylation | Uniprot | |
S1048 | Phosphorylation | Uniprot | |
S1049 | Phosphorylation | Uniprot | |
T1088 | Phosphorylation | Uniprot | |
S1103 | Phosphorylation | Uniprot | |
T1106 | Phosphorylation | Uniprot | |
S1114 | Phosphorylation | Uniprot | |
T1167 | Phosphorylation | Uniprot | |
S1169 | Phosphorylation | Uniprot | |
S1171 | Phosphorylation | Uniprot | |
T1185 | Phosphorylation | Uniprot | |
T1206 | Phosphorylation | Uniprot | |
S1216 | Phosphorylation | Uniprot | |
R1227 | Methylation | Uniprot | |
S1545 | Phosphorylation | Uniprot | |
T1549 | Phosphorylation | Uniprot | |
S1550 | Phosphorylation | Uniprot | |
K1637 | Ubiquitination | Uniprot |
研究背景
Histone methyltransferase that specifically methylates 'Lys-4' of histone H3, when part of the SET1 histone methyltransferase (HMT) complex, but not if the neighboring 'Lys-9' residue is already methylated. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. The non-overlapping localization with SETD1B suggests that SETD1A and SETD1B make non-redundant contributions to the epigenetic control of chromatin structure and gene expression.
Nucleus speckle. Chromosome.
Note: Localizes to a largely non-overlapping set of euchromatic nuclear speckles with SETD1B, suggesting that SETD1A and SETD1B each bind to a unique set of target genes.
Component of the SET1 complex, at least composed of the catalytic subunit (SETD1A or SETD1B), WDR5, WDR82, RBBP5, ASH2L/ASH2, CXXC1/CFP1, HCFC1 and DPY30. Interacts with HCFC1. Interacts with ASH2/ASH2L, CXXC1/CFP1 and RBBP5. Interacts (via the RRM domain) with WDR82. Interacts (via the RRM domain) with hyperphosphorylated C-terminal domain (CTD) of RNA polymerase II large subunit (POLR2A) only in the presence of WDR82. Binds specifically to CTD heptad repeats phosphorylated on 'Ser-5' of each heptad. Interacts with ZNF335. Interacts with SUPT6H. Interacts with NAP1L1 (By similarity). Interacts (via WIN motif) with WDR5.
Belongs to the class V-like SAM-binding methyltransferase superfamily.
研究领域
· Metabolism > Amino acid metabolism > Lysine degradation.
文献引用
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