产品: CAT1 抗体
货号: DF13433
描述: Rabbit polyclonal antibody to CAT1
应用: WB IHC
文献验证: IHC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 67kDa; 68kD(Calculated).
蛋白号: P30825
RRID: AB_2846452

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   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
预测:
Pig(88%), Bovine(%), Horse(%), Sheep(%), Rabbit(%), Dog(%)
克隆:
Polyclonal
特异性:
CAT1 Antibody detects endogenous levels of total CAT1.
RRID:
AB_2846452
引用格式: Affinity Biosciences Cat# DF13433, RRID:AB_2846452.
偶联:
Unconjugated. 130
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Amino acid transporter cationic 1; ATRC1; CAT-1; CaT1; CTR1_HUMAN; Ecotropic retroviral leukemia receptor homolog; Ecotropic retroviral receptor; Ecotropic retrovirus receptor homolog; ERR; HCAT1; High affinity cationic amino acid transporter 1; High-affinity cationic amino acid transporter-1; REC1L; Slc7a1; Solute carrier family 7 (cationic amino acid transporter, y+ system) member 1; Solute carrier family 7 member 1; System Y+ basic amino acid transporter;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
P30825 SL7A1_HUMAN:

Ubiquitous.

序列:
MGCKVLLNIGQQMLRRKVVDCSREETRLSRCLNTFDLVALGVGSTLGAGVYVLAGAVARENAGPAIVISFLIAALASVLAGLCYGEFGARVPKTGSAYLYSYVTVGELWAFITGWNLILSYIIGTSSVARAWSATFDELIGRPIGEFSRTHMTLNAPGVLAENPDIFAVIIILILTGLLTLGVKESAMVNKIFTCINVLVLGFIMVSGFVKGSVKNWQLTEEDFGNTSGRLCLNNDTKEGKPGVGGFMPFGFSGVLSGAATCFYAFVGFDCIATTGEEVKNPQKAIPVGIVASLLICFIAYFGVSAALTLMMPYFCLDNNSPLPDAFKHVGWEGAKYAVAVGSLCALSASLLGSMFPMPRVIYAMAEDGLLFKFLANVNDRTKTPIIATLASGAVAAVMAFLFDLKDLVDLMSIGTLLAYSLVAACVLVLRYQPEQPNLVYQMASTSDELDPADQNELASTNDSQLGFLPEAEMFSLKTILSPKNMEPSKISGLIVNISTSLIAVLIITFCIVTVLGREALTKGALWAVFLLAGSALLCAVVTGVIWRQPESKTKLSFKVPFLPVLPILSIFVNVYLMMQLDQGTWVRFAVWMLIGFIIYFGYGLWHSEEASLDADQARTPDGNLDQCK

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
88
Horse
88
Bovine
88
Sheep
88
Dog
88
Rabbit
88
Chicken
75
Zebrafish
63
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P30825 作为底物

Site PTM Type Enzyme
K4 Ubiquitination
K215 Ubiquitination
N226 N-Glycosylation
K373 Ubiquitination
Y441 Phosphorylation
K484 Ubiquitination
T620 Phosphorylation
C628 S-Nitrosylation
K629 Sumoylation
K629 Ubiquitination

研究背景

功能:

High-affinity, low capacity permease involved in the transport of the cationic amino acids (arginine, lysine and ornithine) in non-hepatic tissues.

细胞定位:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Ubiquitous.

亚基结构:

Interacts with TM4SF5; the interaction is negatively regulated by arginine.

蛋白家族:

Belongs to the amino acid-polyamine-organocation (APC) superfamily. Cationic amino acid transporter (CAT) (TC 2.A.3.3) family.

研究领域

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

文献引用

1). Trichodelphinine A alleviates pulmonary fibrosis by inhibiting collagen synthesis via NOX4-ARG1/TGF-β signaling pathway. Phytomedicine : international journal of phytotherapy and phytopharmacology, 2024 (PubMed: 38870750) [IF=6.7]

2). Metabolomics and transcriptomics joint analysis reveals altered amino acid metabolism in esophageal squamous cell carcinoma. Research Square, 2023

Application: IHC    Species: Human    Sample:

Figure 3. IHC staining showed the up-regulated arginine transporter (CAT1), down-regulated succinic acid synthetase1 (ASS1) in ESCC, and up-regulated ornithine decarboxylase (ODC1) in ESCC (A) The bar graph of the IHC score of the CAT1 (A), ASS1 (B), and ODC1 (C) between cancer and normal tissue, and representative images of IHC staining at a magnification of 40x and 200x.

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