| 产品: | Slap 抗体 |
| 货号: | DF13444 |
| 描述: | Rabbit polyclonal antibody to Slap |
| 应用: | WB |
| 反应: | Human, Mouse, Rat |
| 预测: | Bovine, Sheep, Dog |
| 分子量: | 31kDa; 31kD(Calculated). |
| 蛋白号: | Q13239 |
| RRID: | AB_2846463 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF13444, RRID:AB_2846463.
展开/折叠
hSLAP; SLA 1; sla; SLA1; SLAP 1; SLAP; SLAP-1; SLAP1; SLAP1_HUMAN; Src like adapter; Src like adapter protein 1; Src like adaptor; SRC-like-adapter; Src-like-adapter protein 1;
抗原和靶标
Expressed in lung and fetal brain. Weakly expressed in heart, adult brain, placenta, liver, skeletal muscle, kidney and pancreas.
- Q13239 SLAP1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGNSMKSTPAPAERPLPNPEGLDSDFLAVLSDYPSPDISPPIFRRGEKLRVISDEGGWWKAISLSTGRESYIPGICVARVYHGWLFEGLGRDKAEELLQLPDTKVGSFMIRESETKKGFYSLSVRHRQVKHYRIFRLPNNWYYISPRLTFQCLEDLVNHYSEVADGLCCVLTTPCLTQSTAAPAVRASSSPVTLRQKTVDWRRVSRLQEDPEGTENPLGVDESLFSYGLRESIASYLSLTSEDNTSFDRKKKSISLMYGGSKRKSSFFSSPPYFED
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q13239 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K60 | Ubiquitination | Uniprot | |
| K93 | Ubiquitination | Uniprot | |
| K104 | Ubiquitination | Uniprot | |
| K117 | Ubiquitination | Uniprot | |
| Y120 | Phosphorylation | Uniprot | |
| S189 | Phosphorylation | Uniprot | |
| S190 | Phosphorylation | Uniprot | |
| T193 | Phosphorylation | Uniprot | |
| K197 | Ubiquitination | Uniprot | |
| S205 | Phosphorylation | Uniprot | |
| S235 | Phosphorylation | Uniprot | |
| S238 | Phosphorylation | Uniprot | |
| T240 | Phosphorylation | Uniprot | |
| K252 | Acetylation | Uniprot | |
| K252 | Ubiquitination | Uniprot | |
| S253 | Phosphorylation | Uniprot | |
| S255 | Phosphorylation | Uniprot | |
| Y258 | Phosphorylation | Uniprot | |
| S261 | Phosphorylation | Uniprot | |
| K262 | Acetylation | Uniprot | |
| K262 | Ubiquitination | Uniprot | |
| K264 | Ubiquitination | Uniprot | |
| S266 | Phosphorylation | Uniprot | |
| Y273 | Phosphorylation | Uniprot |
研究背景
Adapter protein, which negatively regulates T-cell receptor (TCR) signaling. Inhibits T-cell antigen-receptor induced activation of nuclear factor of activated T-cells. Involved in the negative regulation of positive selection and mitosis of T-cells. May act by linking signaling proteins such as ZAP70 with CBL, leading to a CBL dependent degradation of signaling proteins.
Cytoplasm. Endosome.
Note: Colocalizes with endosomes.
Expressed in lung and fetal brain. Weakly expressed in heart, adult brain, placenta, liver, skeletal muscle, kidney and pancreas.
Interacts with EPHA2, VAV1, LCP2 and PDGFRB (By similarity). Homodimer. Homodimerization and interaction with phosphorylated CBL occurs via its C-terminal domain. Interacts with phosphorylated proteins ZAP70, CD3Z, SYK and LAT via its SH2 domain.
The C-terminal domain is essential for the homodimerization and the interaction with CBL. While the interaction with CBL is apparently mediated via the hydrophobic region of this domain, the highly charged region is apparently required for the homodimerization.
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