| 产品: | STAM1 抗体 |
| 货号: | DF13448 |
| 描述: | Rabbit polyclonal antibody to STAM1 |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 59kDa; 59kD(Calculated). |
| 蛋白号: | Q92783 |
| RRID: | AB_2846467 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF13448, RRID:AB_2846467.
展开/折叠
HSE1 homolog; Signal transducing adapter molecule 1; signal transducing adaptor molecule (SH3 domain and ITAM motif) 1; STAM; STAM-1; STAM1_HUMAN;
抗原和靶标
- Q92783 STAM1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPLFATNPFDQDVEKATSEMNTAEDWGLILDICDKVGQSRTGPKDCLRSIMRRVNHKDPHVAMQALTLLGACVSNCGKIFHLEVCSRDFASEVSNVLNKGHPKVCEKLKALMVEWTDEFKNDPQLSLISAMIKNLKEQGVTFPAIGSQAAEQAKASPALVAKDPGTVANKKEEEDLAKAIELSLKEQRQQSTTLSTLYPSTSSLLTNHQHEGRKVRAIYDFEAAEDNELTFKAGEIITVLDDSDPNWWKGETHQGIGLFPSNFVTADLTAEPEMIKTEKKTVQFSDDVQVETIEPEPEPAFIDEDKMDQLLQMLQSTDPSDDQPDLPELLHLEAMCHQMGPLIDEKLEDIDRKHSELSELNVKVMEALSLYTKLMNEDPMYSMYAKLQNQPYYMQSSGVSGSQVYAGPPPSGAYLVAGNAQMSHLQSYSLPPEQLSSLSQAVVPPSANPALPSQQTQAAYPNTMVSSVQGNTYPSQAPVYSPPPAATAAAATADVTLYQNAGPNMPQVPNYNLTSSTLPQPGGSQQPPQPQQPYSQKALL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q92783 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S91 | Phosphorylation | Uniprot | |
| K99 | Ubiquitination | Uniprot | |
| K103 | Ubiquitination | Uniprot | |
| K133 | Ubiquitination | Uniprot | |
| K136 | Ubiquitination | Uniprot | |
| K154 | Ubiquitination | Uniprot | |
| S156 | Phosphorylation | Uniprot | |
| K162 | Ubiquitination | Uniprot | |
| K170 | Ubiquitination | Uniprot | |
| K171 | Ubiquitination | Uniprot | |
| K178 | Ubiquitination | Uniprot | |
| S183 | Phosphorylation | Uniprot | |
| K185 | Ubiquitination | Uniprot | |
| T196 | Phosphorylation | Uniprot | |
| Y198 | Phosphorylation | Uniprot | |
| S200 | Phosphorylation | Uniprot | |
| T201 | Phosphorylation | Uniprot | |
| S202 | Phosphorylation | Uniprot | |
| S203 | Phosphorylation | Uniprot | |
| T206 | Phosphorylation | Uniprot | |
| K276 | Ubiquitination | Uniprot | |
| K353 | Ubiquitination | Uniprot | |
| S355 | Phosphorylation | Uniprot | |
| Y371 | Phosphorylation | Uniprot | |
| T372 | Phosphorylation | Uniprot | |
| Y381 | Phosphorylation | Uniprot | |
| S382 | Phosphorylation | Uniprot | |
| Y384 | Phosphorylation | Uniprot |
研究背景
Involved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and MYC induction. May also play a role in T-cell development. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with HGS (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes.
Phosphorylated on Tyr-198. Phosphorylated in response to IL2, IL3, IL4, IL7, CSF2/GM-CSF, EGF and PDGFB. Phosphorylated by activated PDGFRB.
Ubiquitinated by ITCH.
Cytoplasm. Early endosome membrane>Peripheral membrane protein>Cytoplasmic side.
Ubiquitously expressed.
Component of the ESCRT-0 complex composed of STAM or STAM2 and HGS. Probably part of a complex at least composed of HSG, STAM and EPS15. Found in a complex with HGS and E3 ligase ITCH and DTX3L. Interacts with E3 ligase DTX3L; the interaction brings together STAM and HSG, promotes their recruitment to early endosomes and decreases STAM and HGS ubiquitination by ITCH. Interacts with STAMBP/AMSH. Interacts with PDGFRB. Interacts with LITAF; the interaction is direct. Identified in a complex with HGS and LITAF.
The VHS domain mediates high-avidity binding to Lys63-linked and Lys48-linked polyubiquitinated cargos.
Belongs to the STAM family.
研究领域
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway. (View pathway)
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