产品: | KCNQ2 抗体 |
货号: | DF13451 |
描述: | Rabbit polyclonal antibody to KCNQ2 |
应用: | WB IF/ICC |
反应: | Human, Mouse, Rat |
预测: | Pig, Horse, Chicken |
分子量: | 95kDa; 96kD(Calculated). |
蛋白号: | O43526 |
RRID: | AB_2846470 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF13451, RRID:AB_2846470.
抗原和靶标
In adult and fetal brain. Highly expressed in areas containing neuronal cell bodies, low in spinal chord and corpus callosum. Isoform 2 is preferentially expressed in differentiated neurons. Isoform 6 is prominent in fetal brain, undifferentiated neuroblastoma cells and brain tumors.
- O43526 KCNQ2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVQKSRNGGVYPGPSGEKKLKVGFVGLDPGAPDSTRDGALLIAGSEAPKRGSILSKPRAGGAGAGKPPKRNAFYRKLQNFLYNVLERPRGWAFIYHAYVFLLVFSCLVLSVFSTIKEYEKSSEGALYILEIVTIVVFGVEYFVRIWAAGCCCRYRGWRGRLKFARKPFCVIDIMVLIASIAVLAAGSQGNVFATSALRSLRFLQILRMIRMDRRGGTWKLLGSVVYAHSKELVTAWYIGFLCLILASFLVYLAEKGENDHFDTYADALWWGLITLTTIGYGDKYPQTWNGRLLAATFTLIGVSFFALPAGILGSGFALKVQEQHRQKHFEKRRNPAAGLIQSAWRFYATNLSRTDLHSTWQYYERTVTVPMYSSQTQTYGASRLIPPLNQLELLRNLKSKSGLAFRKDPPPEPSPSKGSPCRGPLCGCCPGRSSQKVSLKDRVFSSPRGVAAKGKGSPQAQTVRRSPSADQSLEDSPSKVPKSWSFGDRSRARQAFRIKGAASRQNSEEASLPGEDIVDDKSCPCEFVTEDLTPGLKVSIRAVCVMRFLVSKRKFKESLRPYDVMDVIEQYSAGHLDMLSRIKSLQSRVDQIVGRGPAITDKDRTKGPAEAELPEDPSMMGRLGKVEKQVLSMEKKLDFLVNIYMQRMGIPPTETEAYFGAKEPEPAPPYHSPEDSREHVDRHGCIVKIVRSSSSTGQKNFSAPPAAPPVQCPPSTSWQPQSHPRQGHGTSPVGDHGSLVRIPPPPAHERSLSAYGGGNRASMEFLRQEDTPGCRPPEGNLRDSDTSISIPSVDHEELERSFSGFSISQSKENLDALNSCYAAVAPCAKVRPYIAEGESDTDSDLCTPCGPPPRSATGEGPFGDVGWAGPRK
种属预测
score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O43526 作为底物
Site | PTM Type | Enzyme | Source |
---|---|---|---|
K21 | Ubiquitination | Uniprot | |
K49 | Ubiquitination | Uniprot | |
S52 | Phosphorylation | Uniprot | |
T217 | Phosphorylation | Uniprot | |
S229 | Phosphorylation | Uniprot | |
Y237 | Phosphorylation | Uniprot | |
Y372 | Phosphorylation | Uniprot | |
Y379 | Phosphorylation | Uniprot | |
K398 | Acetylation | Uniprot | |
S438 | Phosphorylation | Uniprot | |
S445 | Phosphorylation | Uniprot | |
S507 | Phosphorylation | Uniprot | |
K556 | Methylation | Uniprot | |
Y562 | Phosphorylation | Uniprot | |
Y571 | Phosphorylation | Uniprot | |
Y644 | Phosphorylation | Uniprot | |
Y658 | Phosphorylation | Uniprot | |
Y755 | Phosphorylation | Uniprot | |
S762 | Phosphorylation | Uniprot | |
Y821 | Phosphorylation | Uniprot | |
S843 | Phosphorylation | Uniprot | |
S855 | Phosphorylation | Uniprot | |
T857 | Phosphorylation | Uniprot |
研究背景
Associates with KCNQ3 to form a potassium channel with essentially identical properties to the channel underlying the native M-current, a slowly activating and deactivating potassium conductance which plays a critical role in determining the subthreshold electrical excitability of neurons as well as the responsiveness to synaptic inputs. Therefore, it is important in the regulation of neuronal excitability. KCNQ2/KCNQ3 current is blocked by linopirdine and XE991, and activated by the anticonvulsant retigabine. As the native M-channel, the potassium channel composed of KCNQ2 and KCNQ3 is also suppressed by activation of the muscarinic acetylcholine receptor CHRM1.
KCNQ2/KCNQ3 heteromeric current can be increased by intracellular cyclic AMP, an effect that depends on phosphorylation of Ser-52 in the N-terminal region.
KCNQ2/KCNQ3 are ubiquitinated by NEDD4L. Ubiquitination leads to protein degradation (Probable). Degradation induced by NEDD4L is inhibited by USP36.
Cell membrane>Multi-pass membrane protein.
In adult and fetal brain. Highly expressed in areas containing neuronal cell bodies, low in spinal chord and corpus callosum. Isoform 2 is preferentially expressed in differentiated neurons. Isoform 6 is prominent in fetal brain, undifferentiated neuroblastoma cells and brain tumors.
Heterotetramer with KCNQ3; form the heterotetrameric M potassium channel. Interacts with calmodulin; the interaction is calcium-independent, constitutive and participates to the proper assembly of a functional heterotetrameric M channel. May associate with KCNE2. Interacts with IQCJ-SCHIP1 (By similarity).
The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
Belongs to the potassium channel family. KQT (TC 1.A.1.15) subfamily. Kv7.2/KCNQ2 sub-subfamily.
研究领域
· Organismal Systems > Nervous system > Cholinergic synapse.
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