| 产品: | Niemann Pick C1 抗体 |
| 货号: | DF13452 |
| 描述: | Rabbit polyclonal antibody to Niemann Pick C1 |
| 应用: | WB |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Horse, Rabbit, Dog |
| 分子量: | 142kDa; 142kD(Calculated). |
| 蛋白号: | O15118 |
| RRID: | AB_2846471 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF13452, RRID:AB_2846471.
展开/折叠
Niemann Pick C1 protein precursor; Niemann Pick disease, type C1; Niemann-Pick C1 protein; NPC; NPC1; NPC1_HUMAN;
抗原和靶标
- O15118 NPC1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTARGLALGLLLLLLCPAQVFSQSCVWYGECGIAYGDKRYNCEYSGPPKPLPKDGYDLVQELCPGFFFGNVSLCCDVRQLQTLKDNLQLPLQFLSRCPSCFYNLLNLFCELTCSPRQSQFLNVTATEDYVDPVTNQTKTNVKELQYYVGQSFANAMYNACRDVEAPSSNDKALGLLCGKDADACNATNWIEYMFNKDNGQAPFTITPVFSDFPVHGMEPMNNATKGCDESVDEVTAPCSCQDCSIVCGPKPQPPPPPAPWTILGLDAMYVIMWITYMAFLLVFFGAFFAVWCYRKRYFVSEYTPIDSNIAFSVNASDKGEASCCDPVSAAFEGCLRRLFTRWGSFCVRNPGCVIFFSLVFITACSSGLVFVRVTTNPVDLWSAPSSQARLEKEYFDQHFGPFFRTEQLIIRAPLTDKHIYQPYPSGADVPFGPPLDIQILHQVLDLQIAIENITASYDNETVTLQDICLAPLSPYNTNCTILSVLNYFQNSHSVLDHKKGDDFFVYADYHTHFLYCVRAPASLNDTSLLHDPCLGTFGGPVFPWLVLGGYDDQNYNNATALVITFPVNNYYNDTEKLQRAQAWEKEFINFVKNYKNPNLTISFTAERSIEDELNRESDSDVFTVVISYAIMFLYISLALGHMKSCRRLLVDSKVSLGIAGILIVLSSVACSLGVFSYIGLPLTLIVIEVIPFLVLAVGVDNIFILVQAYQRDERLQGETLDQQLGRVLGEVAPSMFLSSFSETVAFFLGALSVMPAVHTFSLFAGLAVFIDFLLQITCFVSLLGLDIKRQEKNRLDIFCCVRGAEDGTSVQASESCLFRFFKNSYSPLLLKDWMRPIVIAIFVGVLSFSIAVLNKVDIGLDQSLSMPDDSYMVDYFKSISQYLHAGPPVYFVLEEGHDYTSSKGQNMVCGGMGCNNDSLVQQIFNAAQLDNYTRIGFAPSSWIDDYFDWVKPQSSCCRVDNITDQFCNASVVDPACVRCRPLTPEGKQRPQGGDFMRFLPMFLSDNPNPKCGKGGHAAYSSAVNILLGHGTRVGATYFMTYHTVLQTSADFIDALKKARLIASNVTETMGINGSAYRVFPYSVFYVFYEQYLTIIDDTIFNLGVSLGAIFLVTMVLLGCELWSAVIMCATIAMVLVNMFGVMWLWGISLNAVSLVNLVMSCGISVEFCSHITRAFTVSMKGSRVERAEEALAHMGSSVFSGITLTKFGGIVVLAFAKSQIFQIFYFRMYLAMVLLGATHGLIFLPVLLSYIGPSVNKAKSCATEERYKGTERERLLNF
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O15118 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K49 | Ubiquitination | Uniprot | |
| N70 | N-Glycosylation | Uniprot | |
| K84 | Ubiquitination | Uniprot | |
| S95 | Phosphorylation | Uniprot | |
| N122 | N-Glycosylation | Uniprot | |
| N135 | N-Glycosylation | Uniprot | |
| N158 | N-Glycosylation | Uniprot | |
| K171 | Ubiquitination | Uniprot | |
| N185 | N-Glycosylation | Uniprot | |
| N222 | N-Glycosylation | Uniprot | |
| Y302 | Phosphorylation | Uniprot | |
| S382 | Phosphorylation | Uniprot | |
| S385 | Phosphorylation | Uniprot | |
| S386 | Phosphorylation | Uniprot | |
| K392 | Ubiquitination | Uniprot | |
| Y394 | Phosphorylation | Uniprot | |
| N452 | N-Glycosylation | Uniprot | |
| N459 | N-Glycosylation | Uniprot | |
| N478 | N-Glycosylation | Uniprot | |
| N524 | N-Glycosylation | Uniprot | |
| N557 | N-Glycosylation | Uniprot | |
| N572 | N-Glycosylation | Uniprot | |
| K585 | Ubiquitination | Uniprot | |
| N598 | N-Glycosylation | Uniprot | |
| K822 | Ubiquitination | Uniprot | |
| S826 | Phosphorylation | Uniprot | |
| K903 | Acetylation | Uniprot | |
| N916 | N-Glycosylation | Uniprot | |
| N931 | N-Glycosylation | Uniprot | |
| N961 | N-Glycosylation | Uniprot | |
| N968 | N-Glycosylation | Uniprot | |
| K987 | Ubiquitination | Uniprot | |
| N1064 | N-Glycosylation | Uniprot | |
| K1180 | Ubiquitination | Uniprot | |
| T1270 | Phosphorylation | Uniprot |
研究背景
Intracellular cholesterol transporter which acts in concert with NPC2 and plays an important role in the egress of cholesterol from the endosomal/lysosomal compartment. Unesterified cholesterol that has been released from LDLs in the lumen of the late endosomes/lysosomes is transferred by NPC2 to the cholesterol-binding pocket in the N-terminal domain of NPC1. Cholesterol binds to NPC1 with the hydroxyl group buried in the binding pocket. Binds oxysterol with higher affinity than cholesterol. May play a role in vesicular trafficking in glia, a process that may be crucial for maintaining the structural and functional integrity of nerve terminals (Probable).
(Microbial infection) Acts as an endosomal entry receptor for ebolavirus.
N-glycosylated.
Late endosome membrane>Multi-pass membrane protein. Lysosome membrane>Multi-pass membrane protein.
Interacts (via the second lumenal domain) with NPC2. Interacts with TMEM97. Interacts with TIM1.
(Microbial infection) Interacts with ebolavirus glycoprotein.
A cysteine-rich N-terminal domain and a C-terminal domain containing a di-leucine motif necessary for lysosomal targeting are critical for mobilization of cholesterol from lysosomes.
Belongs to the patched family.
研究领域
· Cellular Processes > Transport and catabolism > Lysosome. (View pathway)
· Organismal Systems > Digestive system > Cholesterol metabolism.
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