产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF13464, RRID:AB_2846483.
展开/折叠
anapc7; Anaphase promoting complex subunit 7; Anaphase-promoting complex subunit 7; APC 7; APC7; APC7_HUMAN; Cyclosome subunit 7; Prediabetic NOD sera reactive autoantigen;
抗原和靶标
- Q9UJX3 APC7_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDPGDAAILESSLRILYRLFESVLPPLPAALQSRMNVIDHVRDMAAAGLHSNVRLLSSLLLTMSNNNPELFSPPQKYQLLVYHADSLFHDKEYRNAVSKYTMALQQKKALSKTSKVRPSTGNSASTPQSQCLPSEIEVKYKMAECYTMLKQDKDAIAILDGIPSRQRTPKINMMLANLYKKAGQERPSVTSYKEVLRQCPLALDAILGLLSLSVKGAEVASMTMNVIQTVPNLDWLSVWIKAYAFVHTGDNSRAISTICSLEKKSLLRDNVDLLGSLADLYFRAGDNKNSVLKFEQAQMLDPYLIKGMDVYGYLLAREGRLEDVENLGCRLFNISDQHAEPWVVSGCHSFYSKRYSRALYLGAKAIQLNSNSVQALLLKGAALRNMGRVQEAIIHFREAIRLAPCRLDCYEGLIECYLASNSIREAMVMANNVYKTLGANAQTLTLLATVCLEDPVTQEKAKTLLDKALTQRPDYIKAVVKKAELLSREQKYEDGIALLRNALANQSDCVLHRILGDFLVAVNEYQEAMDQYSIALSLDPNDQKSLEGMQKMEKEESPTDATQEEDVDDMEGSGEEGDLEGSDSEAAQWADQEQWFGMQ
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9UJX3 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S51 | Phosphorylation | P53350 (PLK1) | Uniprot |
| S57 | Phosphorylation | P53350 (PLK1) | Uniprot |
| S58 | Phosphorylation | Uniprot | |
| T62 | Phosphorylation | Uniprot | |
| S64 | Phosphorylation | P53350 (PLK1) | Uniprot |
| Y77 | Phosphorylation | Uniprot | |
| K91 | Ubiquitination | Uniprot | |
| K99 | Ubiquitination | Uniprot | |
| K107 | Ubiquitination | Uniprot | |
| K115 | Ubiquitination | Uniprot | |
| S119 | Phosphorylation | Uniprot | |
| T120 | Phosphorylation | Uniprot | |
| S123 | Phosphorylation | Uniprot | |
| S125 | Phosphorylation | Uniprot | |
| T126 | Phosphorylation | Uniprot | |
| S129 | Phosphorylation | Uniprot | |
| K139 | Ubiquitination | Uniprot | |
| K153 | Ubiquitination | Uniprot | |
| K181 | Ubiquitination | Uniprot | |
| K193 | Ubiquitination | Uniprot | |
| K263 | Acetylation | Uniprot | |
| K263 | Ubiquitination | Uniprot | |
| K288 | Ubiquitination | Uniprot | |
| K293 | Ubiquitination | Uniprot | |
| Y311 | Phosphorylation | Uniprot | |
| Y313 | Phosphorylation | Uniprot | |
| K353 | Ubiquitination | Uniprot | |
| Y360 | Phosphorylation | Uniprot | |
| K364 | Ubiquitination | Uniprot | |
| K379 | Ubiquitination | Uniprot | |
| S420 | Phosphorylation | Uniprot | |
| S422 | Phosphorylation | Uniprot | |
| Y434 | Phosphorylation | Uniprot | |
| K462 | Ubiquitination | Uniprot | |
| K467 | Acetylation | Uniprot | |
| K467 | Ubiquitination | Uniprot | |
| Y475 | Phosphorylation | Uniprot | |
| K477 | Ubiquitination | Uniprot | |
| K482 | Ubiquitination | Uniprot | |
| K491 | Ubiquitination | Uniprot | |
| K551 | Ubiquitination | Uniprot |
研究背景
Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.
V-shaped homodimer. The mammalian APC/C is composed at least of 14 distinct subunits ANAPC1, ANAPC2, CDC27/APC3, ANAPC4, ANAPC5, CDC16/APC6, ANAPC7, CDC23/APC8, ANAPC10, ANAPC11, CDC26/APC12, ANAPC13, ANAPC15 and ANAPC16 that assemble into a complex of at least 19 chains with a combined molecular mass of around 1.2 MDa; APC/C interacts with FZR1 and FBXO5.
Belongs to the APC7 family.
研究领域
· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)
· Cellular Processes > Cell growth and death > Oocyte meiosis. (View pathway)
· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis. (View pathway)
· Human Diseases > Infectious diseases: Viral > HTLV-I infection.
· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.
限制条款
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