产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF13465, RRID:AB_2846484.
展开/折叠
Core histone macro H2A2.2; Core histone macro-H2A.2; Core histone macroH2A2.2; H2A histone family member Y2; H2AFY2; H2AW_HUMAN; Histone macroH2A2; Macro H2A.2; Macro H2A2; MacroH2A.2; MacroH2A2; mH2A2;
抗原和靶标
- Q9P0M6 H2AW_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSGRSGKKKMSKLSRSARAGVIFPVGRLMRYLKKGTFKYRISVGAPVYMAAVIEYLAAEILELAGNAARDNKKARIAPRHILLAVANDEELNQLLKGVTIASGGVLPRIHPELLAKKRGTKGKSETILSPPPEKRGRKATSGKKGGKKSKAAKPRTSKKSKPKDSDKEGTSNSTSEDGPGDGFTILSSKSLVLGQKLSLTQSDISHIGSMRVEGIVHPTTAEIDLKEDIGKALEKAGGKEFLETVKELRKSQGPLEVAEAAVSQSSGLAAKFVIHCHIPQWGSDKCEEQLEETIKNCLSAAEDKKLKSVAFPPFPSGRNCFPKQTAAQVTLKAISAHFDDSSASSLKNVYFLLFDSESIGIYVQEMAKLDAK
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9P0M6 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K12 | Acetylation | Uniprot | |
| R27 | Methylation | Uniprot | |
| Y31 | Phosphorylation | Uniprot | |
| Y55 | Phosphorylation | Uniprot | |
| K116 | Ubiquitination | Uniprot | |
| K117 | Ubiquitination | Uniprot | |
| K123 | Ubiquitination | Uniprot | |
| S129 | Phosphorylation | Uniprot | |
| S160 | Phosphorylation | Uniprot | |
| S165 | Phosphorylation | Uniprot | |
| S171 | Phosphorylation | Uniprot | |
| S190 | Phosphorylation | Uniprot | |
| S198 | Phosphorylation | Uniprot | |
| T200 | Phosphorylation | Uniprot | |
| K239 | Ubiquitination | Uniprot | |
| K246 | Ubiquitination | Uniprot | |
| S251 | Phosphorylation | Uniprot | |
| S263 | Phosphorylation | Uniprot | |
| S266 | Phosphorylation | Uniprot | |
| K285 | Acetylation | Uniprot | |
| K304 | Acetylation | Uniprot | |
| K332 | Ubiquitination | Uniprot | |
| S342 | Phosphorylation | Uniprot |
研究背景
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in stable X chromosome inactivation.
Nucleus. Chromosome.
Note: Enriched in inactive X chromosome chromatin (PubMed:11331621, PubMed:11262398) and in senescence-associated heterochromatin (PubMed:15621527).
The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers.
研究领域
· Cellular Processes > Cell growth and death > Necroptosis. (View pathway)
· Human Diseases > Substance dependence > Alcoholism.
· Human Diseases > Immune diseases > Systemic lupus erythematosus.
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