产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF13475, RRID:AB_2846494.
展开/折叠
CMTRIB; DFNB89; EC 6.1.1.6; KARS 1; KARS 2; KARS; KARS1; KARS2; KIAA0070; KRS; Lysine tRNA ligase; Lysine--tRNA ligase; LysRS; Lysyl tRNA synthetase; Lysyl-tRNA synthetase; SYK_HUMAN;
抗原和靶标
- Q15046 SYK_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAAVQAAEVKVDGSEPKLSKNELKRRLKAEKKVAEKEAKQKELSEKQLSQATAAATNHTTDNGVGPEEESVDPNQYYKIRSQAIHQLKVNGEDPYPHKFHVDISLTDFIQKYSHLQPGDHLTDITLKVAGRIHAKRASGGKLIFYDLRGEGVKLQVMANSRNYKSEEEFIHINNKLRRGDIIGVQGNPGKTKKGELSIIPYEITLLSPCLHMLPHLHFGLKDKETRYRQRYLDLILNDFVRQKFIIRSKIITYIRSFLDELGFLEIETPMMNIIPGGAVAKPFITYHNELDMNLYMRIAPELYHKMLVVGGIDRVYEIGRQFRNEGIDLTHNPEFTTCEFYMAYADYHDLMEITEKMVSGMVKHITGSYKVTYHPDGPEGQAYDVDFTPPFRRINMVEELEKALGMKLPETNLFETEETRKILDDICVAKAVECPPPRTTARLLDKLVGEFLEVTCINPTFICDHPQIMSPLAKWHRSKEGLTERFELFVMKKEICNAYTELNDPMRQRQLFEEQAKAKAAGDDEAMFIDENFCTALEYGLPPTAGWGMGIDRVAMFLTDSNNIKEVLLFPAMKPEDKKENVATTDTLESTTVGTSV
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q15046 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| K10 | Ubiquitination | Uniprot | |
| S14 | Phosphorylation | Uniprot | |
| K31 | Acetylation | Uniprot | |
| S44 | Phosphorylation | Uniprot | |
| S49 | Phosphorylation | Uniprot | |
| T52 | Phosphorylation | Q16539 (MAPK14) | Uniprot |
| K78 | Ubiquitination | Uniprot | |
| K88 | Acetylation | Uniprot | |
| K88 | Methylation | Uniprot | |
| K127 | Ubiquitination | Uniprot | |
| K141 | Acetylation | Uniprot | |
| K141 | Ubiquitination | Uniprot | |
| R148 | Methylation | Uniprot | |
| K164 | Acetylation | Uniprot | |
| K164 | Ubiquitination | Uniprot | |
| K175 | Acetylation | Uniprot | |
| K175 | Ubiquitination | Uniprot | |
| K190 | Ubiquitination | Uniprot | |
| S207 | Phosphorylation | P27361 (MAPK3) | Uniprot |
| K249 | Ubiquitination | Uniprot | |
| K305 | Ubiquitination | Uniprot | |
| R314 | Methylation | Uniprot | |
| K363 | Ubiquitination | Uniprot | |
| T366 | Phosphorylation | Uniprot | |
| K402 | Acetylation | Uniprot | |
| K402 | Ubiquitination | Uniprot | |
| K407 | Ubiquitination | Uniprot | |
| K421 | Ubiquitination | Uniprot | |
| K430 | Ubiquitination | Uniprot | |
| S470 | Phosphorylation | Uniprot | |
| K492 | Ubiquitination | Uniprot | |
| K493 | Ubiquitination | Uniprot | |
| K517 | Ubiquitination | Uniprot | |
| S561 | Phosphorylation | Uniprot | |
| K578 | Ubiquitination | Uniprot | |
| T587 | Phosphorylation | Uniprot | |
| S590 | Phosphorylation | Uniprot | |
| S596 | Phosphorylation | Uniprot |
研究背景
Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages. Catalyzes the synthesis of the signaling molecule diadenosine tetraphosphate (Ap4A), and thereby mediates disruption of the complex between HINT1 and MITF and the concomitant activation of MITF transcriptional activity.
(Microbial infection) Interacts with HIV-1 virus GAG protein, facilitating the selective packaging of tRNA(3)(Lys), the primer for reverse transcription initiation.
Phosphorylated on a serine residue after mast cell stimulation with immunoglobulin E (IgE).
Cytoplasm>Cytosol. Cytoplasm. Nucleus. Cell membrane>Peripheral membrane protein. Secreted.
Note: Secretion is induced by TNF-alpha (PubMed:15851690). Cytosolic in quiescent mast cells. Translocates into the nucleus in response to mast cell activation by immunoglobulin E (PubMed:23159739).
Mitochondrion.
Homodimer and tetradimer. Part of the multisynthetase complex (MSC), a multisubunit complex that groups tRNA ligases for Arg (RARS), Asp (DARS), Gln (QARS), Ile (IARS), Leu (LARS), Lys (KARS), Met (MARS) the bifunctional ligase for Glu and Pro (EPRS) and the auxiliary subunits AIMP1/p43, AIMP2/p38 and EEF1E1/p18. Interacts with AIMP2 (via N-terminus) and MITF. Interacts with TARSL2.
(Microbial infection) Interacts directly with HIV-1 virus GAG protein.
The N-terminal domain (1-65) of the cytoplasmic isoform is a functional tRNA-binding domain, is required for nuclear localization, is involved in the interaction with DARS, but has a repulsive role in the binding to EEF1A1. A central domain (208-259) is involved in homodimerization and is required for interaction with HIV-1 GAG and incorporation into virions. The C-terminal domain (452-597) is not required for interaction with AIMP2.
Belongs to the class-II aminoacyl-tRNA synthetase family.
研究领域
· Genetic Information Processing > Translation > Aminoacyl-tRNA biosynthesis.
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