| 产品: | AK2 抗体 |
| 货号: | DF13478 |
| 描述: | Rabbit polyclonal antibody to AK2 |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 26kDa; 26kD(Calculated). |
| 蛋白号: | P54819 |
| RRID: | AB_2846497 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF13478, RRID:AB_2846497.
展开/折叠
Adenylate kinase 2; adenylate kinase 2, mitochondrial; Adenylate kinase isoenzyme 2; ADK2; AK 2; ak2; ATP AMP transphosphorylase; ATP-AMP transphosphorylase 2; EC 2.7.4.3; KAD2_HUMAN; mitochondrial;
抗原和靶标
Present in most tissues. Present at high level in heart, liver and kidney, and at low level in brain, skeletal muscle and skin. Present in thrombocytes but not in erythrocytes, which lack mitochondria. Present in all nucleated cell populations from blood, while AK1 is mostly absent. In spleen and lymph nodes, mononuclear cells lack AK1, whereas AK2 is readily detectable. These results indicate that leukocytes may be susceptible to defects caused by the lack of AK2, as they do not express AK1 in sufficient amounts to compensate for the AK2 functional deficits (at protein level).
- P54819 KAD2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAPSVPAAEPEYPKGIRAVLLGPPGAGKGTQAPRLAENFCVCHLATGDMLRAMVASGSELGKKLKATMDAGKLVSDEMVVELIEKNLETPLCKNGFLLDGFPRTVRQAEMLDDLMEKRKEKLDSVIEFSIPDSLLIRRITGRLIHPKSGRSYHEEFNPPKEPMKDDITGEPLIRRSDDNEKALKIRLQAYHTQTTPLIEYYRKRGIHSAIDASQTPDVVFASILAAFSKATCKDLVMFI
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P54819 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| S4 | Phosphorylation | Uniprot | |
| Y12 | Phosphorylation | Uniprot | |
| K14 | Acetylation | Uniprot | |
| K14 | Ubiquitination | Uniprot | |
| K28 | Acetylation | Uniprot | |
| K28 | Ubiquitination | Uniprot | |
| C40 | S-Nitrosylation | Uniprot | |
| C42 | S-Nitrosylation | Uniprot | |
| T46 | Phosphorylation | Uniprot | |
| S56 | Phosphorylation | Uniprot | |
| S58 | Phosphorylation | Uniprot | |
| K62 | Ubiquitination | Uniprot | |
| K72 | Acetylation | Uniprot | |
| S75 | Phosphorylation | Uniprot | |
| K85 | Ubiquitination | Uniprot | |
| T89 | Phosphorylation | Uniprot | |
| C92 | S-Nitrosylation | Uniprot | |
| K93 | Acetylation | Uniprot | |
| K93 | Ubiquitination | Uniprot | |
| K117 | Ubiquitination | Uniprot | |
| S133 | Phosphorylation | Uniprot | |
| K147 | Ubiquitination | Uniprot | |
| S148 | Phosphorylation | Uniprot | |
| S151 | Phosphorylation | Uniprot | |
| Y152 | Phosphorylation | Uniprot | |
| S176 | Phosphorylation | Uniprot | |
| K181 | Acetylation | Uniprot | |
| K181 | Ubiquitination | Uniprot | |
| Y190 | Phosphorylation | Uniprot | |
| T192 | Phosphorylation | Uniprot | |
| T194 | Phosphorylation | Uniprot | |
| T195 | Phosphorylation | Uniprot | |
| Y200 | Phosphorylation | Uniprot | |
| Y201 | Phosphorylation | Uniprot | |
| S208 | Phosphorylation | Uniprot | |
| S213 | Phosphorylation | Uniprot | |
| T215 | Phosphorylation | Uniprot | |
| C232 | S-Nitrosylation | Uniprot | |
| K233 | Ubiquitination | Uniprot |
研究背景
Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism. Adenylate kinase activity is critical for regulation of the phosphate utilization and the AMP de novo biosynthesis pathways. Plays a key role in hematopoiesis.
Mitochondrion intermembrane space.
Present in most tissues. Present at high level in heart, liver and kidney, and at low level in brain, skeletal muscle and skin. Present in thrombocytes but not in erythrocytes, which lack mitochondria. Present in all nucleated cell populations from blood, while AK1 is mostly absent. In spleen and lymph nodes, mononuclear cells lack AK1, whereas AK2 is readily detectable. These results indicate that leukocytes may be susceptible to defects caused by the lack of AK2, as they do not express AK1 in sufficient amounts to compensate for the AK2 functional deficits (at protein level).
Monomer.
Consists of three domains, a large central CORE domain and two small peripheral domains, NMPbind and LID, which undergo movements during catalysis. The LID domain closes over the site of phosphoryl transfer upon ATP binding. Assembling and dissambling the active center during each catalytic cycle provides an effective means to prevent ATP hydrolysis.
Belongs to the adenylate kinase family. AK2 subfamily.
研究领域
· Metabolism > Nucleotide metabolism > Purine metabolism.
· Metabolism > Metabolism of cofactors and vitamins > Thiamine metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
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