Chk1 Antibody - #AF6005

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产品: Chk1 抗体
货号: AF6005
描述: Rabbit polyclonal antibody to Chk1
应用: WB IF/ICC
文献验证: WB, IF/ICC
反应: Human, Monkey
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
蛋白号: O14757
RRID: AB_2834940

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Monkey
克隆:
Polyclonal
特异性:
Chk1 Antibody detects endogenous levels of total Chk1.
RRID:
AB_2834940
引用格式: Affinity Biosciences Cat# AF6005, RRID:AB_2834940.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

C85740; Cell cycle checkpoint kinase; Checkpoint , S. pombe, homolog of, 1; Checkpoint kinase 1; Checkpoint kinase 1 homolog (S. pombe); CHEK 1; Chek1; Chk 1; Chk1; CHK1 checkpoint homolog (S. pombe); CHK1_HUMAN; EC 2.7.11.1; rad27; Serine/threonine protein kinase Chk1; Serine/threonine-protein kinase CHK1; STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae);

抗原和靶标

免疫原:

A synthesized peptide derived from human Chk1, corresponding to a region within the internal amino acids.

基因/基因ID:
CHEK1(1111)
描述:
DNA damage induced protein phosphorylation; regulation of mitotic centrosome separation; regulation of S phase; peptidyl-threonine phosphorylation; DNA repair; chromatin-mediated maintenance of transcription; negative regulation of mitosis;

研究领域

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

· Cellular Processes > Cell growth and death > p53 signaling pathway.   (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

文献引用

1). Hydrogen sulfide alleviates ischemia induced liver injury by repressing the SPHK1/S1P pathway. Annals of Translational Medicine, 2023 (PubMed: 36819566)

Application: WB    Species: Rat    Sample: liver

Figure 1 NaHS decreases endoplasmic reticulum stress and hepatic cell viability under hypoxic conditions. (A) MTT analysis of BRL-3A cell viability after sham, hypoxia, and NaHS treatment. (B) Hoechst staining analysis of BRL-3A cells after sham, hypoxia, and NaHS treatment (magnification, ×200). (C) Left: representative Western blot analysis of the protein expression of CHOP, SPHK1, and S1P. Right: corresponding quantification of the band densities of CHOP, SPHK1, and S1P. (D) Immunofluorescence analysis of CHOP and SPHK1 levels in BRL-3A cells after sham, hypoxia, and NaHS treatment (magnification, ×400). **, P

Application: IF/ICC    Species: Rat    Sample: BRL-3A cells

Figure 1 NaHS decreases endoplasmic reticulum stress and hepatic cell viability under hypoxic conditions. (A) MTT analysis of BRL-3A cell viability after sham, hypoxia, and NaHS treatment. (B) Hoechst staining analysis of BRL-3A cells after sham, hypoxia, and NaHS treatment (magnification, ×200). (C) Left: representative Western blot analysis of the protein expression of CHOP, SPHK1, and S1P. Right: corresponding quantification of the band densities of CHOP, SPHK1, and S1P. (D) Immunofluorescence analysis of CHOP and SPHK1 levels in BRL-3A cells after sham, hypoxia, and NaHS treatment (magnification, ×400). **, P

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