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| 产品: | MTR 抗体 |
| 货号: | DF13489 |
| 描述: | Rabbit polyclonal antibody to MTR |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus |
| 分子量: | 140kDa; 141kD(Calculated). |
| 蛋白号: | Q99707 |
| RRID: | AB_2846508 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF13489, RRID:AB_2846508.
展开/折叠
5-methyltetrahydrofolate homocysteine methyltransferase; 5-methyltetrahydrofolate-homocysteine methyltransferase; cblG; Methionine synthase; methioninesynthase; MS; MTR; MTR1; PRP20; SRM1; Vitamin-B12 dependent methionine synthase;
抗原和靶标
Widely expressed. Expressed at the highest levels in pancreas, heart, brain, skeletal muscle and placenta. Expressed at lower levels in lung, liver and kidney.
- Q99707 METH_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSPALQDLSQPEGLKKTLRDEINAILQKRIMVLDGGMGTMIQREKLNEEHFRGQEFKDHARPLKGNNDILSITQPDVIYQIHKEYLLAGADIIETNTFSSTSIAQADYGLEHLAYRMNMCSAGVARKAAEEVTLQTGIKRFVAGALGPTNKTLSVSPSVERPDYRNITFDELVEAYQEQAKGLLDGGVDILLIETIFDTANAKAALFALQNLFEEKYAPRPIFISGTIVDKSGRTLSGQTGEGFVISVSHGEPLCIGLNCALGAAEMRPFIEIIGKCTTAYVLCYPNAGLPNTFGDYDETPSMMAKHLKDFAMDGLVNIVGGCCGSTPDHIREIAEAVKNCKPRVPPATAFEGHMLLSGLEPFRIGPYTNFVNIGERCNVAGSRKFAKLIMAGNYEEALCVAKVQVEMGAQVLDVNMDDGMLDGPSAMTRFCNLIASEPDIAKVPLCIDSSNFAVIEAGLKCCQGKCIVNSISLKEGEDDFLEKARKIKKYGAAMVVMAFDEEGQATETDTKIRVCTRAYHLLVKKLGFNPNDIIFDPNILTIGTGMEEHNLYAINFIHATKVIKETLPGARISGGLSNLSFSFRGMEAIREAMHGVFLYHAIKSGMDMGIVNAGNLPVYDDIHKELLQLCEDLIWNKDPEATEKLLRYAQTQGTGGKKVIQTDEWRNGPVEERLEYALVKGIEKHIIEDTEEARLNQKKYPRPLNIIEGPLMNGMKIVGDLFGAGKMFLPQVIKSARVMKKAVGHLIPFMEKEREETRVLNGTVEEEDPYQGTIVLATVKGDVHDIGKNIVGVVLGCNNFRVIDLGVMTPCDKILKAALDHKADIIGLSGLITPSLDEMIFVAKEMERLAIRIPLLIGGATTSKTHTAVKIAPRYSAPVIHVLDASKSVVVCSQLLDENLKDEYFEEIMEEYEDIRQDHYESLKERRYLPLSQARKSGFQMDWLSEPHPVKPTFIGTQVFEDYDLQKLVDYIDWKPFFDVWQLRGKYPNRGFPKIFNDKTVGGEARKVYDDAHNMLNTLISQKKLRARGVVGFWPAQSIQDDIHLYAEAAVPQAAEPIATFYGLRQQAEKDSASTEPYYCLSDFIAPLHSGIRDYLGLFAVACFGVEELSKAYEDDGDDYSSIMVKALGDRLAEAFAEELHERVRRELWAYCGSEQLDVADLRRLRYKGIRPAPGYPSQPDHTEKLTMWRLADIEQSTGIRLTESLAMAPASAVSGLYFSNLKSKYFAVGKISKDQVEDYALRKNISVAEVEKWLGPILGYDTD
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q99707 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Phosphorylation | Uniprot | |
| K16 | Ubiquitination | Uniprot | |
| T17 | Phosphorylation | Uniprot | |
| K28 | Ubiquitination | Uniprot | |
| T39 | Phosphorylation | Uniprot | |
| K45 | Ubiquitination | Uniprot | |
| K57 | Ubiquitination | Uniprot | |
| K64 | Ubiquitination | Uniprot | |
| S121 | Phosphorylation | Uniprot | |
| K127 | Ubiquitination | Uniprot | |
| T133 | Phosphorylation | Uniprot | |
| K139 | Ubiquitination | Uniprot | |
| K151 | Ubiquitination | Uniprot | |
| K231 | Ubiquitination | Uniprot | |
| K309 | Ubiquitination | Uniprot | |
| K339 | Ubiquitination | Uniprot | |
| K388 | Ubiquitination | Uniprot | |
| Y395 | Phosphorylation | Uniprot | |
| K466 | Ubiquitination | Uniprot | |
| K475 | Ubiquitination | Uniprot | |
| K484 | Ubiquitination | Uniprot | |
| Y491 | Phosphorylation | Uniprot | |
| Y520 | Phosphorylation | Uniprot | |
| K565 | Ubiquitination | Uniprot | |
| K638 | Ubiquitination | Uniprot | |
| K645 | Ubiquitination | Uniprot | |
| K658 | Ubiquitination | Uniprot | |
| K659 | Ubiquitination | Uniprot | |
| Y677 | Phosphorylation | Uniprot | |
| K681 | Ubiquitination | Uniprot | |
| K685 | Ubiquitination | Uniprot | |
| K699 | Ubiquitination | Uniprot | |
| K700 | Ubiquitination | Uniprot | |
| Y701 | Phosphorylation | Uniprot | |
| K727 | Ubiquitination | Uniprot | |
| K735 | Ubiquitination | Uniprot | |
| K742 | Ubiquitination | Uniprot | |
| K753 | Ubiquitination | Uniprot | |
| T758 | Phosphorylation | Uniprot | |
| T764 | Phosphorylation | Uniprot | |
| T774 | Phosphorylation | Uniprot | |
| K781 | Ubiquitination | Uniprot | |
| K789 | Ubiquitination | Uniprot | |
| K814 | Ubiquitination | Uniprot | |
| K817 | Methylation | Uniprot | |
| K817 | Ubiquitination | Uniprot | |
| K823 | Ubiquitination | Uniprot | |
| K865 | Ubiquitination | Uniprot | |
| K871 | Ubiquitination | Uniprot | |
| S877 | Phosphorylation | Uniprot | |
| Y905 | Phosphorylation | Uniprot | |
| Y913 | Phosphorylation | Uniprot | |
| Y921 | Phosphorylation | Uniprot | |
| K925 | Ubiquitination | Uniprot | |
| Y929 | Phosphorylation | Uniprot | |
| K952 | Ubiquitination | Uniprot | |
| K995 | Ubiquitination | Uniprot | |
| K1000 | Ubiquitination | Uniprot | |
| K1008 | Ubiquitination | Uniprot | |
| Y1010 | Phosphorylation | Uniprot | |
| S1022 | Phosphorylation | Uniprot | |
| K1024 | Ubiquitination | Uniprot | |
| K1071 | Ubiquitination | Uniprot | |
| K1127 | Ubiquitination | Uniprot | |
| R1132 | Methylation | Uniprot | |
| S1155 | Phosphorylation | Uniprot | |
| K1169 | Ubiquitination | Uniprot | |
| K1186 | Ubiquitination | Uniprot | |
| S1206 | Phosphorylation | Uniprot | |
| Y1219 | Phosphorylation | Uniprot | |
| S1221 | Phosphorylation | Uniprot | |
| K1226 | Ubiquitination | Uniprot | |
| K1232 | Ubiquitination | Uniprot | |
| K1235 | Ubiquitination | Uniprot | |
| K1245 | Ubiquitination | Uniprot |
研究背景
Catalyzes the transfer of a methyl group from methyl-cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate (By similarity).
Cytoplasm.
Widely expressed. Expressed at the highest levels in pancreas, heart, brain, skeletal muscle and placenta. Expressed at lower levels in lung, liver and kidney.
Monomer. Dimer. Mainly monomer. Interacts with MTRR.
Modular enzyme with four functionally distinct domains. The isolated Hcy-binding domain catalyzes methyl transfer from free methylcobalamin to homocysteine. The Hcy-binding domain in association with the pterin-binding domain catalyzes the methylation of cob(I)alamin by methyltetrahydrofolate and the methylation of homocysteine. The B12-binding domain binds the cofactor. The AdoMet activation domain binds S-adenosyl-L-methionine. Under aerobic conditions cob(I)alamin can be converted to inactive cob(II)alamin. Reductive methylation by S-adenosyl-L-methionine and flavodoxin regenerates methylcobalamin (By similarity).
Belongs to the vitamin-B12 dependent methionine synthase family.
研究领域
· Metabolism > Amino acid metabolism > Cysteine and methionine metabolism.
· Metabolism > Metabolism of other amino acids > Selenocompound metabolism.
· Metabolism > Metabolism of cofactors and vitamins > One carbon pool by folate.
· Metabolism > Global and overview maps > Metabolic pathways.
· Metabolism > Global and overview maps > Biosynthesis of amino acids.
文献引用
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