| 产品: | RASGRF2 抗体 |
| 货号: | DF13513 |
| 描述: | Rabbit polyclonal antibody to RASGRF2 |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Dog |
| 分子量: | 140kDa; 141kD(Calculated). |
| 蛋白号: | O14827 |
| RRID: | AB_2846532 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF13513, RRID:AB_2846532.
展开/折叠
GRF2; Ras guanine nucleotide exchange factor 2; Ras protein specific guanine nucleotide releasing factor 2; Ras-GRF2; Ras-specific guanine nucleotide-releasing factor 2; RASGRF2; RGRF2_HUMAN;
抗原和靶标
Widely expressed with higher expression in brain, followed by heart, lung, pancreas and kidney. Detected in placenta. Expressed in brain and lung (at protein level).
- O14827 RGRF2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MQKSVRYNEGHALYLAFLARKEGTKRGFLSKKTAEASRWHEKWFALYQNVLFYFEGEQSCRPAGMYLLEGCSCERTPAPPRAGAGQGGVRDALDKQYYFTVLFGHEGQKPLELRCEEEQDGKEWMEAIHQASYADILIEREVLMQKYIHLVQIVETEKIAANQLRHQLEDQDTEIERLKSEIIALNKTKERMRPYQSNQEDEDPDIKKIKKVQSFMRGWLCRRKWKTIVQDYICSPHAESMRKRNQIVFTMVEAESEYVHQLYILVNGFLRPLRMAASSKKPPISHDDVSSIFLNSETIMFLHEIFHQGLKARIANWPTLILADLFDILLPMLNIYQEFVRNHQYSLQVLANCKQNRDFDKLLKQYEANPACEGRMLETFLTYPMFQIPRYIITLHELLAHTPHEHVERKSLEFAKSKLEELSRVMHDEVSDTENIRKNLAIERMIVEGCDILLDTSQTFIRQGSLIQVPSVERGKLSKVRLGSLSLKKEGERQCFLFTKHFLICTRSSGGKLHLLKTGGVLSLIDCTLIEEPDASDDDSKGSGQVFGHLDFKIVVEPPDAAAFTVVLLAPSRQEKAAWMSDISQCVDNIRCNGLMTIVFEENSKVTVPHMIKSDARLHKDDTDICFSKTLNSCKVPQIRYASVERLLERLTDLRFLSIDFLNTFLHTYRIFTTAAVVLGKLSDIYKRPFTSIPVRSLELFFATSQNNRGEHLVDGKSPRLCRKFSSPPPLAVSRTSSPVRARKLSLTSPLNSKIGALDLTTSSSPTTTTQSPAASPPPHTGQIPLDLSRGLSSPEQSPGTVEENVDNPRVDLCNKLKRSIQKAVLESAPADRAGVESSPAADTTELSPCRSPSTPRHLRYRQPGGQTADNAHCSVSPASAFAIATAAAGHGSPPGFNNTERTCDKEFIIRRTATNRVLNVLRHWVSKHAQDFELNNELKMNVLNLLEEVLRDPDLLPQERKAAANILRALSQDDQDDIHLKLEDIIQMTDCMKAECFESLSAMELAEQITLLDHVIFRSIPYEEFLGQGWMKLDKNERTPYIMKTSQHFNDMSNLVASQIMNYADVSSRANAIEKWVAVADICRCLHNYNGVLEITSALNRSAIYRLKKTWAKVSKQTKALMDKLQKTVSSEGRFKNLRETLKNCNPPAVPYLGMYLTDLAFIEEGTPNFTEEGLVNFSKMRMISHIIREIRQFQQTSYRIDHQPKVAQYLLDKDLIIDEDTLYELSLKIEPRLPA
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O14827 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S37 | Phosphorylation | Uniprot | |
| S180 | Phosphorylation | Uniprot | |
| T188 | Phosphorylation | Uniprot | |
| K207 | Acetylation | Uniprot | |
| S465 | Phosphorylation | Uniprot | |
| S471 | Phosphorylation | Uniprot | |
| S484 | Phosphorylation | Uniprot | |
| S536 | Phosphorylation | Uniprot | |
| T630 | Phosphorylation | Uniprot | |
| S633 | Phosphorylation | Uniprot | |
| S718 | Phosphorylation | Uniprot | |
| S726 | Phosphorylation | Uniprot | |
| S737 | Phosphorylation | Uniprot | |
| S738 | Phosphorylation | Uniprot | |
| S746 | Phosphorylation | Uniprot | |
| T748 | Phosphorylation | Uniprot | |
| S749 | Phosphorylation | Uniprot | |
| S753 | Phosphorylation | Uniprot | |
| S776 | Phosphorylation | Uniprot | |
| S852 | Phosphorylation | Uniprot | |
| T855 | Phosphorylation | Uniprot | |
| S1000 | Phosphorylation | Uniprot | |
| S1002 | Phosphorylation | Uniprot | |
| Y1090 | Phosphorylation | Uniprot | |
| S1098 | Phosphorylation | Uniprot | |
| T1111 | Phosphorylation | Uniprot |
研究背景
Functions as a calcium-regulated nucleotide exchange factor activating both Ras and RAC1 through the exchange of bound GDP for GTP. Preferentially activates HRAS in vivo compared to RRAS based on their different types of prenylation. Functions in synaptic plasticity by contributing to the induction of long term potentiation.
Phosphorylated by CDK5; down-regulates RASGRF2-mediated RAC1 activation.
Ubiquitinated upon interaction with Ras. Ubiquitination leads to degradation through the 26S proteasome (By similarity).
Cytoplasm. Cell membrane>Peripheral membrane protein. Endoplasmic reticulum membrane>Peripheral membrane protein.
Note: Translocates to membranes when activated. Found both at cell periphery and along the axon of neurons (By similarity).
Widely expressed with higher expression in brain, followed by heart, lung, pancreas and kidney. Detected in placenta. Expressed in brain and lung (at protein level).
Homooligomer and heterooligomer with RASGRF1. Interacts with Ras and RAC1. Interacts in a calcium-dependent manner with calmodulin (By similarity). Interacts with CDK5R1 and EPB49. Interacts with the AMPA receptor through GRIA1 (By similarity). Interacts with microtubules (By similarity).
The Ras-GEF domain and the N-terminal Ras-GEF domain form a Ras-binding site and mediate Ras activation.
The IQ domain mediates the calcium-dependent interaction with calmodulin but is dispensable for the Ras-GEF activity.
The DH (DBL-homology) domain mediates interaction with RASGRF1 and EPB49 and is required for RAC1 activation.
研究领域
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Ras signaling pathway. (View pathway)
限制条款
产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。
产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。
Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。产品仅供科学研究使用。不用于诊断和治疗。
产品未经授权不得转售。
Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.