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产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF13539, RRID:AB_2846558.
展开/折叠
H1; H1 histone family member 5; H1.5; H15 HUMAN; H15_HUMAN; H1B; H1F5; H1s 3; Hist1h1b; Histone 1 H1b; Histone cluster 1 H1b; Histone H1.5; Histone H1a; Histone H1b; Histone H1s 3; MGC126630; MGC126632;
抗原和靶标
- P16401 H15_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSETAPAETATPAPVEKSPAKKKATKKAAGAGAAKRKATGPPVSELITKAVAASKERNGLSLAALKKALAAGGYDVEKNNSRIKLGLKSLVSKGTLVQTKGTGASGSFKLNKKAASGEAKPKAKKAGAAKAKKPAGATPKKAKKAAGAKKAVKKTPKKAKKPAAAGVKKVAKSPKKAKAAAKPKKATKSPAKPKAVKPKAAKPKAAKPKAAKPKAAKAKKAAAKKK
翻译修饰 - P16401 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Phosphorylation | Uniprot | ||
| S2 | Acetylation | Uniprot | |
| S2 | Phosphorylation | Uniprot | |
| T4 | Phosphorylation | Uniprot | |
| T9 | Phosphorylation | Uniprot | |
| T11 | Phosphorylation | P49841 (GSK3B) , PR:P49841 (hGSK3B) | Uniprot |
| K17 | Ubiquitination | Uniprot | |
| S18 | Phosphorylation | Uniprot | |
| K23 | Acetylation | Uniprot | |
| T25 | Phosphorylation | Uniprot | |
| K26 | Acetylation | Uniprot | |
| K27 | Methylation | Uniprot | |
| K35 | Acetylation | Uniprot | |
| K35 | Ubiquitination | Uniprot | |
| K37 | Methylation | Uniprot | |
| K37 | Ubiquitination | Uniprot | |
| T39 | Phosphorylation | Uniprot | |
| S44 | Phosphorylation | Uniprot | |
| T48 | Phosphorylation | Uniprot | |
| K49 | Acetylation | Uniprot | |
| K49 | Ubiquitination | Uniprot | |
| S54 | Phosphorylation | Uniprot | |
| K55 | Acetylation | Uniprot | |
| K55 | Ubiquitination | Uniprot | |
| S61 | Phosphorylation | Uniprot | |
| K66 | Acetylation | Uniprot | |
| K66 | Ubiquitination | Uniprot | |
| K67 | Ubiquitination | Uniprot | |
| Y74 | Phosphorylation | Uniprot | |
| K78 | Acetylation | Uniprot | |
| K78 | Ubiquitination | Uniprot | |
| S81 | Phosphorylation | Uniprot | |
| K88 | Acetylation | Uniprot | |
| K88 | Ubiquitination | Uniprot | |
| S89 | Phosphorylation | Uniprot | |
| S92 | Phosphorylation | Uniprot | |
| K93 | Acetylation | Uniprot | |
| K93 | Ubiquitination | Uniprot | |
| T95 | Phosphorylation | Uniprot | |
| K100 | Ubiquitination | Uniprot | |
| T102 | Phosphorylation | Uniprot | |
| S105 | Phosphorylation | Uniprot | |
| S107 | Phosphorylation | Uniprot | |
| K109 | Acetylation | Uniprot | |
| K109 | Ubiquitination | Uniprot | |
| K112 | Ubiquitination | Uniprot | |
| K113 | Ubiquitination | Uniprot | |
| S116 | Phosphorylation | Uniprot | |
| K120 | Ubiquitination | Uniprot | |
| K122 | Ubiquitination | Uniprot | |
| K132 | Acetylation | Uniprot | |
| K132 | Ubiquitination | Uniprot | |
| K133 | Acetylation | Uniprot | |
| K133 | Ubiquitination | Uniprot | |
| T138 | Phosphorylation | Uniprot | |
| K140 | Acetylation | Uniprot | |
| K143 | Acetylation | Uniprot | |
| K144 | Acetylation | Uniprot | |
| K149 | Acetylation | Uniprot | |
| T155 | Phosphorylation | Uniprot | |
| K160 | Ubiquitination | Uniprot | |
| K161 | Ubiquitination | Uniprot | |
| K168 | Acetylation | Uniprot | |
| K168 | Ubiquitination | Uniprot | |
| S173 | Phosphorylation | Uniprot | |
| T187 | Phosphorylation | Uniprot | |
| S189 | Phosphorylation | Uniprot | |
| K194 | Ubiquitination | Uniprot | |
| K197 | Ubiquitination | Uniprot | |
| K199 | Acetylation | Uniprot | |
| K199 | Ubiquitination | Uniprot | |
| K209 | Acetylation | Uniprot |
研究背景
Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).
H1 histones are progressively phosphorylated during the cell cycle, becoming maximally phosphorylated during late G2 phase and M phase, and being dephosphorylated sharply thereafter (By similarity). Phosphorylated at Thr-11 by GSK3B during mitosis in prometaphase and dephosphorylated in telophase.
Citrullination at Arg-57 (H1R54ci) by PADI4 takes place within the DNA-binding site of H1 and results in its displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance.
Nucleus. Chromosome.
Note: According to PubMed:15911621 more commonly found in heterochromatin. According to PubMed:10997781 associates with actively transcribed chromatin and not heterochromatin.
Ubiquitous. Expressed in the majority of the cell lines tested and in testis.
The C-terminal domain is required for high-affinity binding to chromatin.
Belongs to the histone H1/H5 family.
文献引用
Application: WB Species: Mice Sample: OSRC2 cells
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