产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF13550, RRID:AB_2846569.
展开/折叠
RNPU1Z; Rnulp70; RPU1; Small nuclear ribonucleoprotein 70 (U1); Small nuclear ribonucleoprotein 70kD polypeptide (RNP antigen); Small nuclear ribonucleoprotein 70kDa (U1); Small nuclear ribonucleoprotein 70kDa polypeptide (RNP antigen); snRNP70; Snrp 70; SNRP70; U1 70K; U1 small nuclear ribonucleoprotein 70 kDa; U1 small nuclear ribonucleoprotein polypeptide A; U1 snRNP 70 kDa; U170K; U1AP; U1AP1; U1RNP;
抗原和靶标
- P08621 RU17_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTQFLPPNLLALFAPRDPIPYLPPLEKLPHEKHHNQPYCGIAPYIREFEDPRDAPPPTRAETREERMERKRREKIERRQQEVETELKMWDPHNDPNAQGDAFKTLFVARVNYDTTESKLRREFEVYGPIKRIHMVYSKRSGKPRGYAFIEYEHERDMHSAYKHADGKKIDGRRVLVDVERGRTVKGWRPRRLGGGLGGTRRGGADVNIRHSGRDDTSRYDERPGPSPLPHRDRDRDRERERRERSRERDKERERRRSRSRDRRRRSRSRDKEERRRSRERSKDKDRDRKRRSSRSRERARRERERKEELRGGGGDMAEPSEAGDAPPDDGPPGELGPDGPDGPEEKGRDRDRERRRSHRSERERRRDRDRDRDRDREHKRGERGSERGRDEARGGGGGQDNGLEGLGNDSRDMYMESEGGDGYLAPENGYLMEAAPE
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P08621 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T2 | Acetylation | Uniprot | |
| K27 | Ubiquitination | Uniprot | |
| Y38 | Phosphorylation | Uniprot | |
| C39 | S-Nitrosylation | Uniprot | |
| T84 | Phosphorylation | Uniprot | |
| K87 | Ubiquitination | Uniprot | |
| K103 | Acetylation | Uniprot | |
| K103 | Ubiquitination | Uniprot | |
| Y112 | Phosphorylation | Uniprot | |
| S117 | Phosphorylation | Uniprot | |
| K118 | Acetylation | Uniprot | |
| K118 | Ubiquitination | Uniprot | |
| Y126 | Phosphorylation | Uniprot | |
| K130 | Methylation | Uniprot | |
| K130 | Ubiquitination | Uniprot | |
| S140 | Phosphorylation | Uniprot | |
| Y146 | Phosphorylation | Uniprot | |
| Y151 | Phosphorylation | Uniprot | |
| K162 | Acetylation | Uniprot | |
| R180 | Methylation | Uniprot | |
| K185 | Ubiquitination | Uniprot | |
| T199 | Phosphorylation | Uniprot | |
| R201 | Methylation | Uniprot | |
| S211 | Phosphorylation | Uniprot | |
| S217 | Phosphorylation | Uniprot | |
| Y219 | Phosphorylation | Uniprot | |
| S226 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S257 | Phosphorylation | Uniprot | |
| S259 | Phosphorylation | Uniprot | |
| S266 | Phosphorylation | Uniprot | |
| S268 | Phosphorylation | Uniprot | |
| S277 | Phosphorylation | Uniprot | |
| S281 | Phosphorylation | Uniprot | |
| S293 | Phosphorylation | Uniprot | |
| S295 | Phosphorylation | Uniprot | |
| R310 | Methylation | Uniprot | |
| S320 | Phosphorylation | Uniprot | |
| K346 | Methylation | Uniprot | |
| K346 | Ubiquitination | Uniprot | |
| S357 | Phosphorylation | Uniprot | |
| S360 | Phosphorylation | Uniprot | |
| R393 | Methylation | Uniprot | |
| S410 | Phosphorylation | Uniprot | |
| Y414 | Phosphorylation | Uniprot | |
| S417 | Phosphorylation | Uniprot |
研究背景
Component of the spliceosomal U1 snRNP, which is essential for recognition of the pre-mRNA 5' splice-site and the subsequent assembly of the spliceosome. SNRNP70 binds to the loop I region of U1-snRNA.
Truncated isoforms that lack the RRM domain cannot bind U1-snRNA.
Truncated isoforms that lack the RRM domain cannot bind U1-snRNA.
The N-terminus is blocked.
Extensively phosphorylated on serine residues in the C-terminal region.
Nucleus speckle. Nucleus>Nucleoplasm.
Note: Colocalizes with SCNM1 and LUC7L2 in nuclear speckles.
Component of the U1 snRNP. The U1 snRNP is composed of the U1 snRNA and the 7 core Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP, and at least three U1 snRNP-specific proteins SNRNP70/U1-70K, SNRPA/U1-A and SNRPC/U1-C. Interacts with SCNM1 (By similarity). Found in a pre-mRNA splicing complex with SFRS4, SFRS5, SNRNP70, SNRPA1, SRRM1 and SRRM2. Found in a pre-mRNA exonic splicing enhancer (ESE) complex with SNRNP70, SNRPA1, SRRM1 and TRA2B/SFRS10. Interacts with dephosphorylated SFRS13A and SFPQ. Interacts with NUDT21/CPSF5, CPSF6, SCAF11, and ZRANB2. Interacts with GEMIN5. Interacts with FUS.
The RRM domain mediates interaction with U1 RNA.
研究领域
· Genetic Information Processing > Transcription > Spliceosome.
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