DBT Antibody - #DF13569

>>买2送1 进行中>>
>>满百送 进行中>>
>>赠送JD卡 进行中>>
>>3倍积分 进行中>>
(1)   (9)  
产品: DBT 抗体
货号: DF13569
描述: Rabbit polyclonal antibody to DBT
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
分子量: 53kDa; 53kD(Calculated).
蛋白号: P11182
RRID: AB_2846588

浏览相似产品>>

   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

联系销售
相关下载
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Zebrafish(86%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
DBT Antibody detects endogenous levels of total DBT.
RRID:
AB_2846588
引用格式: Affinity Biosciences Cat# DF13569, RRID:AB_2846588.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

BCATE2; BCKAD E2 subunit; Branched chain acyltransferase, E2 component; Dihydrolipoamide branched chain transacylase (E2 component of branched chain keto acid dehydrogenase complex; maple syrup urine disease); Dihydrolipoamide branched chain transacylase; Dihydrolipoamide branched chain transacylase E2; Dihydrolipoyl transacylase; Dihydrolipoyllysine residue (2 methylpropanoyl)transferase; E2; E2 component of branched chain alpha keto acid dehydrogenase complex; E2B; Lipoamide acyltransferase component of mitochondrial branched chain alpha keto acid dehydrogenase complex; Mitochondrial branched chain alpha keto acid dehydrogenase transacylase subunit (E2b);

抗原和靶标

免疫原:
Uniprot:

P11182(ODB2_HUMAN) >>Visit HPA database.

基因/基因ID:
DBT(1629)
序列:
MAAVRMLRTWSRNAGKLICVRYFQTCGNVHVLKPNYVCFFGYPSFKYSHPHHFLKTTAALRGQVVQFKLSDIGEGIREVTVKEWYVKEGDTVSQFDSICEVQSDKASVTITSRYDGVIKKLYYNLDDIAYVGKPLVDIETEALKDSEEDVVETPAVSHDEHTHQEIKGRKTLATPAVRRLAMENNIKLSEVVGSGKDGRILKEDILNYLEKQTGAILPPSPKVEIMPPPPKPKDMTVPILVSKPPVFTGKDKTEPIKGFQKAMVKTMSAALKIPHFGYCDEIDLTELVKLREELKPIAFARGIKLSFMPFFLKAASLGLLQFPILNASVDENCQNITYKASHNIGIAMDTEQGLIVPNVKNVQICSIFDIATELNRLQKLGSVGQLSTTDLTGGTFTLSNIGSIGGTFAKPVIMPPEVAIGALGSIKAIPRFNQKGEVYKAQIMNVSWSADHRVIDGATMSRFSNLWKSYLENPAFMLLDLK

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Rabbit
100
Zebrafish
86
Pig
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P11182 作为底物

Site PTM Type Enzyme
T56 Phosphorylation
T57 Phosphorylation
T80 Phosphorylation
T153 Phosphorylation
T171 Phosphorylation
K202 Ubiquitination
T213 Phosphorylation
S220 Phosphorylation
K289 Methylation
K295 Acetylation
T392 Phosphorylation
S403 Phosphorylation
K435 Acetylation
K435 Ubiquitination
K440 Acetylation
S447 Phosphorylation
S469 Phosphorylation

研究背景

功能:

The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3). Within this complex, the catalytic function of this enzyme is to accept, and to transfer to coenzyme A, acyl groups that are generated by the branched-chain alpha-keto acid decarboxylase component.

细胞定位:

Mitochondrion matrix.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Forms a 24-polypeptide structural core with octahedral symmetry.

蛋白家族:

Belongs to the 2-oxoacid dehydrogenase family.

研究领域

· Metabolism > Amino acid metabolism > Valine, leucine and isoleucine degradation.

· Metabolism > Carbohydrate metabolism > Propanoate metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

文献引用

1). A comprehensive analysis of the prognostic value and immune infiltration of low expression DBT in clear cell renal cell carcinoma. Frontiers in Pharmacology, 2022 (PubMed: 36299888) [IF=5.6]

Application: WB    Species: Human    Sample: HK-2 cells

FIGURE 2 DBT protein expression is low in ccRCC. (A) The histogram obtained from the analysis of data from the Clinical Proteomic Tumor Analysis Consortium (CPTAC) on the UALCAN website shows significantly lower expression of the DBT protein in ccRCC tissues versus normal tissues (p = 1.31015316430268E-57). (B) The results of western blotting of A498, 786-O, ACHN, Caki-1, and OSRC-2 cell lysates showed lower protein expression of DBT in ccRCC cells compared to HK-2 cells, and the histogram quantifying the results also indicated a statistically significant difference. (C) Similarly, DBT protein expression was lower in renal clear cell carcinoma tissues than in matched pairs of paraneoplastic normal tissues, and the histogram quantification confirmed this finding. (D) Immunohistochemical results from the HPA database showed that DBT expression in ccRCC was mainly in the nucleus, whereas in normal renal tissue, it was in normal tubular epithelial cells. (E) This result was also validated by the immunohistochemical results of 2 pairs of ccRCC tissues from our study centre.

限制条款

产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）

产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.