| 产品: | KMT5C/SUV4-20h2 抗体 |
| 货号: | DF13570 |
| 描述: | Rabbit polyclonal antibody to KMT5C/SUV4-20h2 |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Zebrafish, Bovine, Horse, Sheep, Dog |
| 分子量: | 52kDa,60kDa; 52kD(Calculated). |
| 蛋白号: | Q86Y97 |
| RRID: | AB_2846589 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF13570, RRID:AB_2846589.
展开/折叠
FLJ98627; Histone lysine N methyltransferase SUV420H2; Histone-lysine N-methyltransferase SUV420H2; KMT5C; Lysine N methyltransferase 5C; Lysine N-methyltransferase 5C; MGC2705; Su(var)4 20 homolog 2; Su(var)4-20 homolog 2; Suppressor of variegation 4 20 homolog 2; Suppressor of variegation 4-20 homolog 2; Suv4 20h2; Suv4-20h2; SUV420H2; SV422_HUMAN;
抗原和靶标
- Q86Y97 KMT5C_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGPDRVTARELCENDDLATSLVLDPYLGFRTHKMNVSPVPPLRRQQHLRSALETFLRQRDLEAAYRALTLGGWTARYFQSRGPRQEAALKTHVYRYLRAFLPESGFTILPCTRYSMETNGAKIVSTRAWKKNEKLELLVGCIAELREADEGLLRAGENDFSIMYSTRKRSAQLWLGPAAFINHDCKPNCKFVPADGNAACVKVLRDIEPGDEVTCFYGEGFFGEKNEHCECHTCERKGEGAFRTRPREPALPPRPLDKYQLRETKRRLQQGLDSGSRQGLLGPRACVHPSPLRRDPFCAACQPLRLPACSARPDTSPLWLQWLPQPQPRVRPRKRRRPRPRRAPVLSTHHAARVSLHRWGGCGPHCRLRGEALVALGQPPHARWAPQQDWHWARRYGLPYVVRVDLRRLAPAPPATPAPAGTPGPILIPKQALAFAPFSPPKRLRLVVSHGSIDLDVGGEEL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q86Y97 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T69 | Phosphorylation | Uniprot | |
| S80 | Phosphorylation | Uniprot | |
| Y96 | Phosphorylation | Uniprot | |
| S104 | Phosphorylation | Uniprot | |
| T107 | Phosphorylation | Uniprot | |
| T112 | Phosphorylation | Uniprot | |
| K122 | Ubiquitination | Uniprot | |
| K202 | Ubiquitination | Uniprot | |
| Y259 | Phosphorylation | Uniprot | |
| S274 | Phosphorylation | Uniprot | |
| S290 | Phosphorylation | Uniprot | |
| T416 | Phosphorylation | Uniprot | |
| T422 | Phosphorylation | Uniprot | |
| S439 | Phosphorylation | Uniprot | |
| S449 | Phosphorylation | Uniprot | |
| S452 | Phosphorylation | Uniprot |
研究背景
Histone methyltransferase that specifically methylates monomethylated 'Lys-20' (H4K20me1) and dimethylated 'Lys-20' (H4K20me2) of histone H4 to produce respectively dimethylated 'Lys-20' (H4K20me2) and trimethylated 'Lys-20' (H4K20me3) and thus regulates transcription and maintenance of genome integrity. In vitro also methylates unmodified 'Lys-20' (H4K20me0) of histone H4 and nucleosomes. H4 'Lys-20' trimethylation represents a specific tag for epigenetic transcriptional repression. Mainly functions in pericentric heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin in these regions. KMT5C is targeted to histone H3 via its interaction with RB1 family proteins (RB1, RBL1 and RBL2) (By similarity). Facilitates TP53BP1 foci formation upon DNA damage and proficient non-homologous end-joining (NHEJ)-directed DNA repair by catalyzing the di- and trimethylation of 'Lys-20' of histone H4. May play a role in class switch reconbination by catalyzing the di- and trimethylation of 'Lys-20' of histone H4 (By similarity).
Nucleus. Chromosome.
Note: Associated with pericentric heterochromatin. CBX1 and CBX5 are required for the localization to pericentric heterochromatin (By similarity).
Homodimer (By similarity). Interacts with HP1 proteins CBX1, CBX3 and CBX5. Interacts with RB1 family proteins RB1, RBL1 and RBL2 (By similarity).
Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. Suvar4-20 subfamily.
研究领域
· Metabolism > Amino acid metabolism > Lysine degradation.
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