| 产品: | NAPE-PLD 抗体 |
| 货号: | DF13571 |
| 描述: | Rabbit polyclonal antibody to NAPE-PLD |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse, Rat, Monkey |
| 预测: | Pig, Bovine, Horse, Rabbit |
| 分子量: | 45kDa; 46kD(Calculated). |
| 蛋白号: | Q6IQ20 |
| RRID: | AB_2846590 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF13571, RRID:AB_2846590.
展开/折叠
C7orf18; DKFZp781D1098; FMP30; Mbldc1; N acyl phosphatidylethanolamine hydrolyzing phospholipase D; N acyl phosphatidylethanolamine phospholipase D; N-acyl phosphatidylethanolamine phospholipase D; N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D; NAPE hydrolyzing phospholipase D; NAPE-hydrolyzing phospholipase D; NAPE-PLD; NAPEP_HUMAN; NAPEPLD;
抗原和靶标
Widely expressed. Highest expression in brain, kidney and testis (at protein level). Expressed in adipose tissue (at protein level).
- Q6IQ20 NAPEP_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDENESNQSLMTSSQYPKEAVRKRQNSARNSGASDSSRFSRKSFKLDYRLEEDVTKSKKGKDGRFVNPWPTWKNPSIPNVLRWLIMEKDHSSVPSSKEELDKELPVLKPYFITNPEEAGVREAGLRVTWLGHATVMVEMDELIFLTDPIFSSRASPSQYMGPKRFRRSPCTISELPPIDAVLISHNHYDHLDYNSVIALNERFGNELRWFVPLGLLDWMQKCGCENVIELDWWEENCVPGHDKVTFVFTPSQHWCKRTLMDDNKVLWGSWSVLGPWNRFFFAGDTGYCPAFEEIGKRFGPFDLAAIPIGAYEPRWFMKYQHVDPEEAVRIHTDVQTKKSMAIHWGTFALANEHYLEPPVKLNEALERYGLNAEDFFVLKHGESRYLNNDDENF
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q6IQ20 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| S43 | Phosphorylation | Uniprot | |
| K59 | Methylation | Uniprot | |
| K73 | Ubiquitination | Uniprot | |
| K97 | Ubiquitination | Uniprot | |
| K102 | Ubiquitination | Uniprot | |
| K296 | Ubiquitination | Uniprot |
研究背景
Hydrolyzes N-acyl-phosphatidylethanolamines (NAPEs) to produce N-acylethanolamines (NAEs) and phosphatidic acid. Responsible for the generation of these bioactive fatty acid ethanolamides (FAEs), including anandamide (N-arachidonoylethanolamine), the ligand of cannabinoid and vanilloid receptors. As a regulator of lipid metabolism in the adipose tissue, mediates the crosstalk between adipocytes, gut microbiota and immune cells to control body temperature and weight. In particular, regulates energy homeostasis by promoting cold-induced brown or beige adipocyte differentiation program to generate heat from fatty acids and glucose (By similarity).
Golgi apparatus membrane>Peripheral membrane protein. Early endosome membrane>Peripheral membrane protein. Nucleus envelope. Nucleus>Nucleoplasm.
Note: Localized in the proximity of the cellular membranes likely through interaction with membrane phospholipids.
Widely expressed. Highest expression in brain, kidney and testis (at protein level). Expressed in adipose tissue (at protein level).
Homodimer. Bile acids promote the assembly of inactive monomers into an active dimer and enable catalysis.
Belongs to the NAPE-PLD family.
研究领域
· Organismal Systems > Nervous system > Retrograde endocannabinoid signaling. (View pathway)
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