DHCR7 Antibody | Affinity Biosciences LTD|亲科生物官网

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Western blot analysis of extracts from HepG2 cells, using DHCR7 Antibody.

FIG 1 (A) Cholesterol synthesis pathway.

产品:	DHCR7 抗体
货号:	DF13577
描述:	Rabbit polyclonal antibody to DHCR7
应用:	WB IF/ICC
反应:	Human, Mouse, Rat
预测:	Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
分子量:	54kDa; 54kD(Calculated).
蛋白号:	Q9UBM7
RRID:	AB_2846596

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阻断肽(封闭肽)是特异性结合目标抗体和阻断抗体结合的多肽。这些多肽包含抗体识别的抗原表位。与阻断肽结合的抗体不再与靶蛋白上的表位结合。例如，在免疫印迹(WB)和免疫组化(IHC)中，通过比较被阻断抗体和未阻断抗体的染色，可以看到哪种染色是特异性的。

规格	价格	库存
100ul	RMB¥ 2300	现货
200ul	RMB¥ 3000	现货

货期: 当天发货

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实验方案

产品描述

来源:

Rabbit

应用:

IF/ICC 1:100-1:500, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:

Human,Mouse,Rat

预测:

Pig(100%), Zebrafish(88%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(88%), Xenopus(100%)

克隆:

Polyclonal

特异性:

DHCR7 Antibody detects endogenous levels of total DHCR7.

RRID:

AB_2846596
引用格式: Affinity Biosciences Cat# DF13577, RRID:AB_2846596.

偶联:

Unconjugated.

纯化:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

别名:

展开/折叠

7 dehydrocholesterol reductase; 7 DHC reductase; Delta 7 dehydrocholesterol reductase; Putative sterol reductase SR 2; SLOS; Sterol delta 7 reductase; Sterol Delta;

抗原和靶标

免疫原:

Uniprot:

Q9UBM7(DHCR7_HUMAN) >>Visit HPA database.

基因/基因ID:

DHCR7(1717)

表达:

Q9UBM7 DHCR7_HUMAN:

Widely expressed. Most abundant in adrenal gland, liver, testis, and brain.

序列:

Q9UBM7 DHCR7_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAAKSQPNIPKAKSLDGVTNDRTASQGQWGRAWEVDWFSLASVIFLLLFAPFIVYYFIMACDQYSCALTGPVVDIVTGHARLSDIWAKTPPITRKAAQLYTLWVTFQVLLYTSLPDFCHKFLPGYVGGIQEGAVTPAGVVNKYQINGLQAWLLTHLLWFANAHLLSWFSPTIIFDNWIPLLWCANILGYAVSTFAMVKGYFFPTSARDCKFTGNFFYNYMMGIEFNPRIGKWFDFKLFFNGRPGIVAWTLINLSFAAKQRELHSHVTNAMVLVNVLQAIYVIDFFWNETWYLKTIDICHDHFGWYLGWGDCVWLPYLYTLQGLYLVYHPVQLSTPHAVGVLLLGLVGYYIFRVANHQKDLFRRTDGRCLIWGRKPKVIECSYTSADGQRHHSKLLVSGFWGVARHFNYVGDLMGSLAYCLACGGGHLLPYFYIIYMAILLTHRCLRDEHRCASKYGRDWERYTAAVPYRLLPGIF

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

100

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q9UBM7 作为底物

Q9UBM7

Site	PTM Type	Source
K4	Ubiquitination	Uniprot
S5	Phosphorylation	Uniprot
K13	Ubiquitination	Uniprot
S14	Phosphorylation	Uniprot
T19	Phosphorylation	Uniprot
S25	Phosphorylation	Uniprot
K88	Ubiquitination	Uniprot
T135	Phosphorylation	Uniprot
S205	Phosphorylation	Uniprot
K231	Ubiquitination	Uniprot
K358	Ubiquitination	Uniprot
K376	Ubiquitination	Uniprot
C380	S-Nitrosylation	Uniprot
Y382	Phosphorylation	Uniprot
S397	Phosphorylation	Uniprot
K454	Ubiquitination	Uniprot

研究背景

Q9UBM7_DHCR7_HUMAN

功能:

Production of cholesterol by reduction of C7-C8 double bond of 7-dehydrocholesterol (7-DHC).

细胞定位:

Endoplasmic reticulum membrane>Multi-pass membrane protein.

组织特异性:

Widely expressed. Most abundant in adrenal gland, liver, testis, and brain.

亚基结构:

Interacts with DHCR24; this interaction regulates DHCR7 activity.

蛋白家族:

Belongs to the ERG4/ERG24 family.

研究领域

· Metabolism > Lipid metabolism > Steroid biosynthesis.

· Metabolism > Global and overview maps > Metabolic pathways.

文献引用

1). Augmentation of 3β-hydroxysteroid-Δ24 Reductase (DHCR24) Expression Induced by Bovine Viral Diarrhea Virus Infection Facilitates Viral Replication via Promoting Cholesterol Synthesis. Journal of Virology, 2022 (PubMed: 36468862) [IF=5.4]

Application: WB Species: bovine Sample: bovine cells

FIG 1 (A) Cholesterol synthesis pathway. (B) Heat map of the key genes (transcriptomic data) and corresponding proteins (proteomic data) involved in lipid metabolism in bovine viral diarrhea virus (BVDV)-infected bovine cells. (C, D) Identification of differentially expressed genes by quantitative reverse transcription-PCR (qRT-PCR) (C) and corresponding proteins by Western blotting (D). HMGCS1, 3- hydroxy-3-methylglutaryl-coenzyme A synthase 1; HMGCR, 3-hydroxy-3-methylglutaryl-coenzyme A reductase; MVK, mevalonate kinase; PMVK, phosphomevalonate kinase; MVD, diphosphomevalonate decarboxylase; FDPS, farnesyl diphosphate synthase; FDFT1, farnesyl diphosphate farnesyltransferase 1; SQLE, squalene epoxidase; GGPS, geranylgeranyl pyrophosphate synthase; LSS, lanosterol synthase; D8D7I, 3β-hydroxysterol-Δ8,7-isomerase; C5SD, 3β-hydroxysterol-C5 desaturase; DHCR7, 7-dehydrocholesterol reductase; DHCR24, 3β-hydroxysteroid-Δ24 reductase; SCD1, stearoyl-CoA desaturase; FASN, fatty acid synthase; ACACA, acetyl coenzyme A carboxylase alpha.

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DHCR7 Antibody - #DF13577

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