产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF13582, RRID:AB_2846601.
展开/折叠
ADIP; ADIP_HUMAN; Afadin and alpha actinin binding protein; Afadin DIL domain interacting protein; Afadin DIL domain-interacting protein; Afadin- and alpha-actinin-binding protein; FLJ10848; KIAA0923; MGC75026; OTTHUMP00000011574; SSX 2 interacting protein; SSX2 interacting protein; SSX2-interacting protein; SSX2IP; Synovial sarcoma X breakpoint 2 interacting protein;
抗原和靶标
Widely expressed, with the highest expression in brain, intermediate expression in kidney, testis, spinal cord, liver, heart, lung, skeletal muscle, ovary, fetal liver and fetal brain, and little to no expression in pancreas and spleen. All specific brain regions showed intermediate to high expression, with highest expression in amygdala. Also expressed in fetal tissues, mainly in liver and brain.
- Q9Y2D8 ADIP_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGDWMTVTDPGLSSESKTISQYTSETKMSPSSLYSQQVLCSSIPLSKNVHSFFSAFCTEDNIEQSISYLDQELTTFGFPSLYEESKGKETKRELNIVAVLNCMNELLVLQRKNLLAQENVETQNLKLGSDMDHLQSCYSKLKEQLETSRREMIGLQERDRQLQCKNRNLHQLLKNEKDEVQKLQNIIASRATQYNHDMKRKEREYNKLKERLHQLVMNKKDKKIAMDILNYVGRADGKRGSWRTGKTEARNEDEMYKILLNDYEYRQKQILMENAELKKVLQQMKKEMISLLSPQKKKPRERVDDSTGTVISDVEEDAGELSRESMWDLSCETVREQLTNSIRKQWRILKSHVEKLDNQVSKVHLEGFNDEDVISRQDHEQETEKLELEIQQCKEMIKTQQQLLQQQLATAYDDDTTSLLRDCYLLEEKERLKEEWSLFKEQKKNFERERRSFTEAAIRLGLERKAFEEERASWLKQQFLNMTTFDHQNSENVKLFSAFSGSSDWDNLIVHSRQPQKKPHSVSNGSPVCMSKLTKSLPASPSTSDFCQTRSCISEHSSINVLNITAEEIKPNQVGGECTNQKWSVASRPGSQEGCYSGCSLSYTNSHVEKDDLP
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9Y2D8 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T18 | Phosphorylation | Uniprot | |
| K112 | Ubiquitination | Uniprot | |
| K126 | Ubiquitination | Uniprot | |
| Y138 | Phosphorylation | Uniprot | |
| K142 | Ubiquitination | Uniprot | |
| K174 | Ubiquitination | Uniprot | |
| K182 | Ubiquitination | Uniprot | |
| K219 | Ubiquitination | Uniprot | |
| Y231 | Phosphorylation | Uniprot | |
| K257 | Ubiquitination | Uniprot | |
| K268 | Ubiquitination | Uniprot | |
| S290 | Phosphorylation | Uniprot | |
| S293 | Phosphorylation | Uniprot | |
| S306 | Phosphorylation | Uniprot | |
| S312 | Phosphorylation | Uniprot | |
| S325 | Phosphorylation | Uniprot | |
| S341 | Phosphorylation | Uniprot | |
| K350 | Ubiquitination | Uniprot | |
| K355 | Ubiquitination | Uniprot | |
| K362 | Ubiquitination | Uniprot | |
| K394 | Ubiquitination | Uniprot | |
| K398 | Ubiquitination | Uniprot | |
| K429 | Ubiquitination | Uniprot | |
| K433 | Ubiquitination | Uniprot | |
| K440 | Ubiquitination | Uniprot | |
| S452 | Phosphorylation | Uniprot | |
| T454 | Phosphorylation | Uniprot | |
| K476 | Ubiquitination | Uniprot | |
| S502 | Phosphorylation | Uniprot | |
| S526 | Phosphorylation | Uniprot | |
| K535 | Ubiquitination | Uniprot | |
| S536 | Phosphorylation | Uniprot | |
| S540 | Phosphorylation | Uniprot | |
| S542 | Phosphorylation | Uniprot | |
| K582 | Acetylation | Uniprot | |
| S591 | Phosphorylation | Uniprot |
研究背景
Belongs to an adhesion system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). May connect the nectin-afadin and E-cadherin-catenin system through alpha-actinin and may be involved in organization of the actin cytoskeleton at AJs through afadin and alpha-actinin (By similarity). Involved in cell movement: localizes at the leading edge of moving cells in response to PDGF and is required for the formation of the leading edge and the promotion of cell movement, possibly via activation of Rac signaling (By similarity). Acts as a centrosome maturation factor, probably by maintaining the integrity of the pericentriolar material and proper microtubule nucleation at mitotic spindle poles. The function seems to implicate at least in part WRAP73; the SSX2IP:WRAP73 complex is proposed to act as regulator of spindle anchoring at the mitotic centrosome. Involved in ciliogenesis. It is required for targeted recruitment of the BBSome, CEP290, RAB8, and SSTR3 to the cilia.
Cell junction>Adherens junction. Nucleus. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome>Centriolar satellite. Cytoplasm>Cytoskeleton>Cilium basal body.
Note: Not found at cell-matrix AJs.
Widely expressed, with the highest expression in brain, intermediate expression in kidney, testis, spinal cord, liver, heart, lung, skeletal muscle, ovary, fetal liver and fetal brain, and little to no expression in pancreas and spleen. All specific brain regions showed intermediate to high expression, with highest expression in amygdala. Also expressed in fetal tissues, mainly in liver and brain.
Interacts with afadin and alpha-actinin (By similarity). Interacts with VAV2 (By similarity). Interacts with SSX2 and SSX3. Does not interact with SSX1 and SSX4. Interacts with PCM1 (By similarity). Interacts with WRAP73.
Both the N-terminal (up to position 79) and the C-terminal (from position 304) sequences are required for interaction with SSX2.
Belongs to the ADIP family.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Adherens junction. (View pathway)
限制条款
产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。
产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。
Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。产品仅供科学研究使用。不用于诊断和治疗。
产品未经授权不得转售。
Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.