KLF2 Antibody - #DF13602

Figure 6 FGF2 reduces oxidative stress and ferroptosis via KLF2-NFE2L2 pathway in vivo. (A) Skeletal muscle specimens were used for the KLF2 and NFE2L2 WB assay. (B) Protein quantification of KLF2 and NFE2L2 from (A), normalised to GAPDH band density and histone 3 band density, respectively. (C) Skeletal muscle specimens showing HO-1, SOD1, SLC7A11, 4-HNE, and GPX4 on a WB assay. (D) Quantification of the GAPDH band density-normalized HO-1 and SOD1 protein levels from (C). (E) Quantification of protein level of SLC7A11, 4-HNE and GPX4 from (C) with normalized to GAPDH band density. (F) DAPI staining shows the nuclei in these micrographs of skeletal muscle slices that have been immunostained for KLF2 and CD31. Scale bars: 100 μm. (G) Micrographs of skeletal muscle slices with NFE2L2 and CD31 immunostaining; DAPI labelling shows nuclei. Scale bars: 100 μm. (H) Quantification of cells that co-express KLF2 and CD31; proportions of co-expressing cells to all CD31-positive cells are shown. (I) Quantification of NFE2L2 and CD31 double-positive cells, the ratio of co-expressing cells to all CD31-positive cells is shown. (J) Micrographs of skeletal muscle slices with nuclei identified by DAPI labelling and DHE and 4-HNE staining. Scale bars: 100 μm. Based on the immunofluorescence data in (J), the ROS level was quantified in (K). Based on the immunofluorescence results in (J), 4-HNE fluorescence intensity was quantified in (L). (M) Content of Fe2+ was plotted as a histogram. (N) The concentration of MDA is shown as a histogram. (O) The concentration of GSH is shown as a histogram. Data are provided as mean ± SD (n = 3-5 per group). Significance: ns, not significant; *P < 0.05.

Figure 3 KLF2 inhibits ccRCC migration in vitro and KLF2 protein expression is decreased in high pathological grade tissue (II + III). (A) Expression of KLF2 mRNA in ccRCC and normal HK-2 cells. (B) Western blotting was used for assessment of KLF2 protein levels in ccRCC and normal HK-2 cells. (C) Western blotting assessment of KLF2 OE in 769-P and 786-O cells. (D) Migration of 769-P and 786-O cells after wounding (magnification, x40). (E) compared with NC. (F) Immunohistochemistry and (G) analysis of the KLF2 expression in ccRCC tissue (n=20). **P<0.01 vs. stage I and ***P<0.001 vs. NC. KLF, Krüppel-like factor; ccRCC, clear cell renal cell carcinoma; NC, negative control; OE, overexpression.