产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF13611, RRID:AB_2846630.
展开/折叠
3 N(4) ethenocytosine glycosylase; G/5 fluorouracil mismatch glycosylase with biphasic kinetics; G/T mismatch glycosylase; G/U mismatch glycosylase; MBD 4; MBD4; MBD4_HUMAN; MED 1; MED1; Methyl CpG binding domain protein 4; Methyl CpG binding endonuclease 1; Methyl CpG binding protein MBD4; Methyl-CpG-binding domain protein 4; Methyl-CpG-binding endonuclease 1; Methyl-CpG-binding protein MBD4; Mismatch specific DNA N glycosylase; Mismatch-specific DNA N-glycosylase; Putative methyl CpG binding protein;
抗原和靶标
- O95243 MBD4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGTTGLESLSLGDRGAAPTVTSSERLVPDPPNDLRKEDVAMELERVGEDEEQMMIKRSSECNPLLQEPIASAQFGATAGTECRKSVPCGWERVVKQRLFGKTAGRFDVYFISPQGLKFRSKSSLANYLHKNGETSLKPEDFDFTVLSKRGIKSRYKDCSMAALTSHLQNQSNNSNWNLRTRSKCKKDVFMPPSSSSELQESRGLSNFTSTHLLLKEDEGVDDVNFRKVRKPKGKVTILKGIPIKKTKKGCRKSCSGFVQSDSKRESVCNKADAESEPVAQKSQLDRTVCISDAGACGETLSVTSEENSLVKKKERSLSSGSNFCSEQKTSGIINKFCSAKDSEHNEKYEDTFLESEEIGTKVEVVERKEHLHTDILKRGSEMDNNCSPTRKDFTGEKIFQEDTIPRTQIERRKTSLYFSSKYNKEALSPPRRKAFKKWTPPRSPFNLVQETLFHDPWKLLIATIFLNRTSGKMAIPVLWKFLEKYPSAEVARTADWRDVSELLKPLGLYDLRAKTIVKFSDEYLTKQWKYPIELHGIGKYGNDSYRIFCVNEWKQVHPEDHKLNKYHDWLWENHEKLSLS
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O95243 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K36 | Ubiquitination | Uniprot | |
| K56 | Ubiquitination | Uniprot | |
| Y109 | Phosphorylation | Uniprot | |
| S112 | Phosphorylation | Uniprot | |
| K117 | Ubiquitination | Uniprot | |
| K137 | Ubiquitination | Uniprot | |
| K148 | Ubiquitination | Uniprot | |
| S165 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| K215 | Ubiquitination | Uniprot | |
| K232 | Acetylation | Uniprot | |
| K234 | Acetylation | Uniprot | |
| K239 | Acetylation | Uniprot | |
| K239 | Sumoylation | Uniprot | |
| K239 | Ubiquitination | Uniprot | |
| S253 | Phosphorylation | Uniprot | |
| S255 | Phosphorylation | Uniprot | |
| S262 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| K270 | Sumoylation | Uniprot | |
| K281 | Ubiquitination | Uniprot | |
| S304 | Phosphorylation | Uniprot | |
| S308 | Phosphorylation | Uniprot | |
| S316 | Phosphorylation | Uniprot | |
| S318 | Phosphorylation | Uniprot | |
| S321 | Phosphorylation | Uniprot | |
| S325 | Phosphorylation | Uniprot | |
| T329 | Phosphorylation | Uniprot | |
| S330 | Phosphorylation | Uniprot | |
| K335 | Ubiquitination | Uniprot | |
| K368 | Sumoylation | Uniprot | |
| K368 | Ubiquitination | Uniprot | |
| K377 | Ubiquitination | Uniprot | |
| S387 | Phosphorylation | Uniprot | |
| K397 | Ubiquitination | Uniprot | |
| S415 | Phosphorylation | Uniprot | |
| S428 | Phosphorylation | Uniprot | |
| T439 | Phosphorylation | Uniprot | |
| K484 | Ubiquitination | Uniprot | |
| K504 | Ubiquitination | Uniprot | |
| K518 | Ubiquitination | Uniprot | |
| K526 | Ubiquitination | Uniprot | |
| K529 | Ubiquitination | Uniprot | |
| K554 | Ubiquitination | Uniprot | |
| K562 | Ubiquitination | Uniprot | |
| K576 | Ubiquitination | Uniprot |
研究背景
Mismatch-specific DNA N-glycosylase involved in DNA repair. Has thymine glycosylase activity and is specific for G:T mismatches within methylated and unmethylated CpG sites. Can also remove uracil or 5-fluorouracil in G:U mismatches. Has no lyase activity. Was first identified as methyl-CpG-binding protein.
Nucleus.
Interacts with MLH1.
研究领域
· Genetic Information Processing > Replication and repair > Base excision repair.
限制条款
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