产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF13613, RRID:AB_2846632.
展开/折叠
Aes 1; Aes 2; aes; AES_HUMAN; Aes1; Aes2; Amino enhancer of split; Amino terminal enhancer of split; Amino-terminal enhancer of split; Esp 1; Esp1; Gp130 associated protein GAM; Gp130-associated protein GAM; GRG 5; GRG; GRG protein; Grg-5; GRG5; Groucho-related protein 5; Protein ESP 1; Protein ESP1; Protein GRG; TLE 5; TLE5;
抗原和靶标
Found predominantly in muscle, heart and Placenta. In fetal tissues, abundantly expressed in the heart, lung, kidney, brain and liver.
- Q08117 TLE5_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MMFPQSRHSGSSHLPQQLKFTTSDSCDRIKDEFQLLQAQYHSLKLECDKLASEKSEMQRHYVMYYEMSYGLNIEMHKQAEIVKRLNGICAQVLPYLSQEHQQQVLGAIERAKQVTAPELNSIIRQQLQAHQLSQLQALALPLTPLPVGLQPPSLPAVSAGTGLLSLSALGSQAHLSKEDKNGHDGDTHQEDDGEKSD
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q08117 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| R7 | Methylation | Uniprot | |
| S9 | Phosphorylation | Uniprot | |
| S11 | Phosphorylation | Uniprot | |
| K19 | Ubiquitination | Uniprot | |
| S25 | Phosphorylation | Uniprot | |
| K30 | Ubiquitination | Uniprot | |
| K44 | Ubiquitination | Uniprot | |
| K49 | Ubiquitination | Uniprot | |
| K54 | Ubiquitination | Uniprot | |
| Y69 | Phosphorylation | Uniprot | |
| K83 | Ubiquitination | Uniprot | |
| K112 | Ubiquitination | Uniprot | |
| T143 | Phosphorylation | Uniprot | |
| S196 | Phosphorylation | Uniprot |
研究背景
Transcriptional corepressor. Acts as dominant repressor towards other family members. Inhibits NF-kappa-B-regulated gene expression. May be required for the initiation and maintenance of the differentiated state. Essential for the transcriptional repressor activity of SIX3 during retina and lens development.
Ubiquitinated by XIAP/BIRC4.
Nucleus.
Found predominantly in muscle, heart and Placenta. In fetal tissues, abundantly expressed in the heart, lung, kidney, brain and liver.
Homooligomer and heterooligomer with other family members. Binds TCF7 (By similarity). Binds the NF-kappa-B subunit RELA. Interacts with PHF12. Interacts (via Q domain) with SIX3. Interacts with SIX6.
Lacks the C-terminal WD repeats.
Belongs to the WD repeat Groucho/TLE family.
限制条款
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