SNAP25 Antibody - #AF0254

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产品: SNAP25 抗体
货号: AF0254
描述: Rabbit polyclonal antibody to SNAP25
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
分子量: 25kDa; 23kD(Calculated).
蛋白号: P60880
RRID: AB_2833429

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 50ul RMB¥ 1250 现货
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产品描述

来源:
Rabbit
应用:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(100%), Xenopus(86%)
克隆:
Polyclonal
特异性:
SNAP25 Antibody detects endogenous levels of total SNAP25.
RRID:
AB_2833429
引用格式: Affinity Biosciences Cat# AF0254, RRID:AB_2833429.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

bA416N4.2; Bdr; CMS18; dJ1068F16.2; FLJ23079; HGNC:11132; MGC105414; MGC139754; Resistance to inhibitors of cholinesterase 4 homolog; RIC 4; RIC4; SEC 9; SEC9; SNAP 25; SNAP; SNAP-25; SNAP-25B; SNAP25; SNP 25; SNP25; SNP25_HUMAN; sp; SUP; Super protein; Synaptosomal associated 25 kDa protein; Synaptosomal associated protein; Synaptosomal associated protein 25; Synaptosomal associated protein 25kDa; Synaptosomal-associated 25 kDa protein; Synaptosomal-associated protein 25; Synaptosomal-associated protein, 25-KD;

抗原和靶标

免疫原:
Uniprot:

P60880(SNP25_HUMAN) >>Visit HPA database.

基因/基因ID:
SNAP25(6616)
表达:
P60880 SNP25_HUMAN:

Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum.

描述:
SNAP-25 a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Part of the SNARE core complex containing SNAP25, VAMP2 and syntaxin 1A. Associates with proteins involved in vesicle docking and membrane fusion. Two alternatively spliced isoforms have been described.
序列:
MAEDADMRNELEEMQRRADQLADESLESTRRMLQLVEESKDAGIRTLVMLDEQGEQLERIEEGMDQINKDMKEAEKNLTDLGKFCGLCVCPCNKLKSSDAYKKAWGNNQDGVVASQPARVVDEREQMAISGGFIRRVTNDARENEMDENLEQVSGIIGNLRHMALDMGNEIDTQNRQIDRIMEKADSNKTRIDEANQRATKMLGSG

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Xenopus
86
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P60880 作为底物

Site PTM Type Enzyme
S28 Phosphorylation
T29 Phosphorylation
K40 Ubiquitination
K103 Ubiquitination
T138 Phosphorylation P17252 (PRKCA)
S187 Phosphorylation P17252 (PRKCA)

研究背景

功能:

t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF. Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 in pancreatic beta cells.

翻译修饰:

Palmitoylated. Cys-85 appears to be the main site, and palmitoylation is required for membrane association (By similarity).

(Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type A (BoNT/A, botA) which hydrolyzes the 197-Gln-|-Arg-198 bond and inhibits neurotransmitter release.

(Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type C (BoNT/C) which hydrolyzes the 198-Arg-|-Ala-199 bond and inhibits neurotransmitter release. C.botulinum type C only rarely infects humans.

(Microbial infection) Targeted and hydrolyzed by C.botulinum neurotoxin type E (BoNT/E) which hydrolyzes the 180-Arg-|-Ile-181 bond and inhibits neurotransmitter release.

细胞定位:

Cytoplasm>Perinuclear region. Cell membrane>Lipid-anchor. Cell junction>Synapse>Synaptosome. Photoreceptor inner segment.
Note: Membrane association requires palmitoylation. Expressed throughout cytoplasm, concentrating at the perinuclear region. Colocalizes with KCNB1 at the cell membrane (By similarity). Colocalizes with PLCL1 at the cell membrane (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum.

亚基结构:

Part of the SNARE core complex containing SNAP25, VAMP2 and STX1A; this complex binds CPLX1. Found in a complex containing SYT1, SV2B and syntaxin-1 (By similarity). Found in a ternary complex with STX1A and VAMP8 (By similarity). Interacts with HSC70 and with SYT9, forming a complex with DNAJC5 (By similarity). The interaction with SYT9 is inhibited in presence of calcium (By similarity). Isoform 1 and isoform 2 interact with BLOC1S6. Interacts with CENPF (By similarity). Interacts with EQTN (By similarity). Interacts with HGS (By similarity). Interacts with KCNB1 (via N-terminus); reduces the voltage-dependent potassium channel KCNB1 activity in pancreatic beta cells (By similarity). Interacts with OTOF (By similarity). Interacts with RIMS1 (By similarity). Interacts with SNAPIN (By similarity). Interacts with STXBP6 (By similarity). Interacts with TRIM9 (By similarity). Interacts with ZDHHC13 (via ANK repeats) (By similarity). Interacts with ZDHHC17 (via ANK repeats). Associates with the BLOC-1 complex. Interacts with PLCL1 (via C2 domain) (By similarity). Interacts with PRRT2; this interaction may impair the formation of the SNARE complex. Interacts with alpha-synuclein/SNCA. Interacts with PRPH2 (By similarity). Interacts with ROM1 (By similarity). Interacts with STX3 (By similarity).

蛋白家族:

Belongs to the SNAP-25 family.

研究领域

· Organismal Systems > Nervous system > Synaptic vesicle cycle.

· Organismal Systems > Endocrine system > Insulin secretion.   (View pathway)

文献引用

1). Phosphorylation at Ser 727 Increases STAT3 Interaction with PKCε Regulating Neuron–Glia Crosstalk via IL-6-Mediated Hyperalgesia In Vivo and In Vitro. MEDIATORS OF INFLAMMATION, 2022 (PubMed: 35125963) [IF=4.6]

Application: WB    Species: Rat    Sample: spinal cord tissues

Figure 7 Ser727 of STAT3 increased its interaction with PKCε. (a) Endogenous PKCε was immunoprecipitated from cell lysates, and immune complexes and total cell lysates were analyzed by Western blot analysis with PKCε and STAT3 antibodies. Endogenous immune PKCε/STAT3 complexes were detected in the rat spinal cord tissues. (b) IL-6 promoter-firefly luciferase reporter plasmid (0.5 μg), PKCε (1 μg), and STAT3 (1 μg) were cotransfected overnight into HEK293 cells. The transfected cells were incubated with 1 μg/mL lipopolysaccharide (LPS) for 12 h. Interleukin-6 promoter activity increased by STAT3 was further enhanced by PKCε and STAT3, indicating that PKCε improved the ability of STAT3 to bind to IL-6 promoter. Data are shown as means ± SD (n = 6). ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001; one-way ANOVA followed by Bonferroni tests. (c, d) HKE293 cells were transfected with GFP, GFP-PKCε, Flag, Flag-STAT3, and phosphomimetic and dephosphomimetic mutants of STAT3, and then, immunoprecipitants were assayed. Protein complexes were detected using an anti-GFP antibody (c), and then, relative PKCε binding to STAT3 was quantified (d). Data are presented as means ± SD (n = 3). ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001; one-way ANOVA followed by Bonferroni tests.
2). CHIP Decline Is Associated With Isoflurane-Induced Neurodegeneration in Aged Mice. Frontiers in Neuroscience, 2022 (PubMed: 35368262) [IF=4.3]

Application: WB    Species: Mice    Sample: brain tissue

FIGURE 5 Ubiquitin and synaptic protein expression changed in isoflurane-exposed aged mice. (A) Ubiquitin expression was decreased in the cortex brain tissue in the ISO-15 group, while there was no significant difference in synaptic protein SNAP25 expression in the cortex (n = 6, *p < 0.05). (B) In the brain tissue of cortex, there was no significant difference in the expression level of synapsin I and the phosphorylation level of synapsin I S9 and S605 between CON group, ISO-12.5 group, and ISO-15 group. In the brain tissue of the hippocampus, the expression level of synapsin I and the phosphorylation level of synapsin I S9 and S605 were decreased in both the ISO-12.5 group and ISO-15 group, compared with the CON group, and the expression level of CHIP had a dramatic decline in the ISO-15 group (n = 6, *p < 0.05).

Application: WB    Species: mouse    Sample: N2a cells

FIGURE 6 | Decreased CHIP expression altered synaptic protein expression and phosphorylation in N2a cells. (B) Statistical bar chart to show that CHIP, synapsin I, SNAP25, and PSD95 expression were present (n = 6, **p < 0.01, ***p < 0.001).

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