产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF13625, RRID:AB_2846644.
展开/折叠
DCP2; DCP2 decapping enzyme homolog (S. cerevisiae); DCP2, S. cerevisiae, homolog of; DCP2_HUMAN; decapping enzyme 2, S. cerevisiae, homolog of; decapping enzyme homolog (S. cerevisiae); FLJ33245; hDpc; m7GpppN-mRNA hydrolase; mRNA decapping enzyme 2; mRNA-decapping enzyme 2; Nucleoside diphosphate-linked moiety X motif 20; nudix (nucleoside diphosphate linked moiety X)-type motif 20; Nudix motif 20; NUDT20;
抗原和靶标
- Q8IU60 DCP2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
METKRVEIPGSVLDDLCSRFILHIPSEERDNAIRVCFQIELAHWFYLDFYMQNTPGLPQCGIRDFAKAVFSHCPFLLPQGEDVEKVLDEWKEYKMGVPTYGAIILDETLENVLLVQGYLAKSGWGFPKGKVNKEEAPHDCAAREVFEETGFDIKDYICKDDYIELRINDQLARLYIIPGIPKDTKFNPKTRREIRNIEWFSIEKLPCHRNDMTPKSKLGLAPNKFFMAIPFIRPLRDWLSRRFGDSSDSDNGFSSTGSTPAKPTVEKLSRTKFRHSQQLFPDGSPGDQWVKHRQPLQQKPYNNHSEMSDLLKGKNQSMRGNGRKQYQDSPNQKKRTNGLQPAKQQNSLMKCEKKLHPRKLQDNFETDAVYDLPSSSEDQLLEHAEGQPVACNGHCKFPFSSRAFLSFKFDHNAIMKILDL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q8IU60 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K85 | Ubiquitination | Uniprot | |
| K91 | Ubiquitination | Uniprot | |
| Y93 | Phosphorylation | Uniprot | |
| Y100 | Phosphorylation | Uniprot | |
| K130 | Ubiquitination | Uniprot | |
| K154 | Ubiquitination | Uniprot | |
| K159 | Ubiquitination | Uniprot | |
| Y162 | Phosphorylation | Uniprot | |
| K204 | Ubiquitination | Uniprot | |
| T213 | Phosphorylation | Uniprot | |
| K215 | Acetylation | Uniprot | |
| K217 | Ubiquitination | Uniprot | |
| S246 | Phosphorylation | Uniprot | |
| S247 | Phosphorylation | Uniprot | |
| S249 | Phosphorylation | Uniprot | |
| S254 | Phosphorylation | Uniprot | |
| S276 | Phosphorylation | Uniprot | |
| S284 | Phosphorylation | Uniprot | |
| K291 | Ubiquitination | Uniprot | |
| K299 | Ubiquitination | Uniprot | |
| S308 | Phosphorylation | Uniprot | |
| K312 | Methylation | Uniprot | |
| K312 | Ubiquitination | Uniprot | |
| K314 | Methylation | Uniprot | |
| R319 | Methylation | Uniprot | |
| Y326 | Phosphorylation | Uniprot | |
| S329 | Phosphorylation | Uniprot | |
| S347 | Phosphorylation | Uniprot | |
| Y370 | Phosphorylation | Uniprot | |
| S374 | Phosphorylation | Uniprot | |
| S375 | Phosphorylation | Uniprot | |
| S376 | Phosphorylation | Uniprot | |
| S406 | Phosphorylation | Uniprot | |
| K416 | Ubiquitination | Uniprot |
研究背景
Decapping metalloenzyme that catalyzes the cleavage of the cap structure on mRNAs. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Plays a role in replication-dependent histone mRNA degradation. Has higher activity towards mRNAs that lack a poly(A) tail. Has no activity towards a cap structure lacking an RNA moiety. The presence of a N(6)-methyladenosine methylation at the second transcribed position of mRNAs (N(6),2'-O-dimethyladenosine cap; m6A(m)) provides resistance to DCP2-mediated decapping. Blocks autophagy in nutrient-rich conditions by repressing the expression of ATG-related genes through degradation of their transcripts.
Phosphorylated at ser-249 in a MTOR-dependent manner.
Cytoplasm>P-body. Nucleus.
Note: Predominantly cytoplasmic, in processing bodies (PB) (PubMed:15273322). A minor amount is nuclear (PubMed:15273322).
Expressed in brain and testis. Not detected in heart (at protein level).
Found in a mRNA decay complex with LSM1, LSM3, LSM4, EXOSC2, EXOSC4, EXOSC10, PARN, XRN1, CNOT6, UPF1, UPF2 and UPF3B. Forms a complex with DCP1A, EDC3, DDX6 and EDC4/HEDLS, within this complex directly interacts with EDC4/HEDLS. Interacts with DPC1B. Interacts (via N-terminus and C-terminus) with TRIM21 (via N-terminus and C-terminus). Associates with polysomes. Interacts with LIMD1, WTIP and AJUBA. Interacts with DDX17 in an RNA-dependent manner. Interacts with ZC3HAV1. Interacts with APOBEC3G in an RNA-dependent manner. Interacts with ZFP36L1 (via N-terminus). Interacts with NBDY.
Belongs to the Nudix hydrolase family. DCP2 subfamily.
研究领域
· Genetic Information Processing > Folding, sorting and degradation > RNA degradation.
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