产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF13630, RRID:AB_2846649.
展开/折叠
A1S9; A1S9 protein; A1S9T and BN75 temperature sensitivity complementing; A1S9T; A1ST; AMCX1; CFAP124; CTD-2522E6.1; GXP 1; GXP1; MGC4781; POC20; POC20 centriolar protein homolog; Protein A1S9; SMAX2; Uba1; UBA1, ubiquitin-activating enzyme E1 homolog A; UBA1_HUMAN; UBA1A; UBE 1; UBE 1X; UBE1; UBE1X; Ubiquitin activating enzyme E1; Ubiquitin-activating enzyme E1; Ubiquitin-like modifier-activating enzyme 1;
抗原和靶标
- P22314 UBA1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSSSPLSKKRRVSGPDPKPGSNCSPAQSVLSEVPSVPTNGMAKNGSEADIDEGLYSRQLYVLGHEAMKRLQTSSVLVSGLRGLGVEIAKNIILGGVKAVTLHDQGTAQWADLSSQFYLREEDIGKNRAEVSQPRLAELNSYVPVTAYTGPLVEDFLSGFQVVVLTNTPLEDQLRVGEFCHNRGIKLVVADTRGLFGQLFCDFGEEMILTDSNGEQPLSAMVSMVTKDNPGVVTCLDEARHGFESGDFVSFSEVQGMVELNGNQPMEIKVLGPYTFSICDTSNFSDYIRGGIVSQVKVPKKISFKSLVASLAEPDFVVTDFAKFSRPAQLHIGFQALHQFCAQHGRPPRPRNEEDAAELVALAQAVNARALPAVQQNNLDEDLIRKLAYVAAGDLAPINAFIGGLAAQEVMKACSGKFMPIMQWLYFDALECLPEDKEVLTEDKCLQRQNRYDGQVAVFGSDLQEKLGKQKYFLVGAGAIGCELLKNFAMIGLGCGEGGEIIVTDMDTIEKSNLNRQFLFRPWDVTKLKSDTAAAAVRQMNPHIRVTSHQNRVGPDTERIYDDDFFQNLDGVANALDNVDARMYMDRRCVYYRKPLLESGTLGTKGNVQVVIPFLTESYSSSQDPPEKSIPICTLKNFPNAIEHTLQWARDEFEGLFKQPAENVNQYLTDPKFVERTLRLAGTQPLEVLEAVQRSLVLQRPQTWADCVTWACHHWHTQYSNNIRQLLHNFPPDQLTSSGAPFWSGPKRCPHPLTFDVNNPLHLDYVMAAANLFAQTYGLTGSQDRAAVATFLQSVQVPEFTPKSGVKIHVSDQELQSANASVDDSRLEELKATLPSPDKLPGFKMYPIDFEKDDDSNFHMDFIVAASNLRAENYDIPSADRHKSKLIAGKIIPAIATTTAAVVGLVCLELYKVVQGHRQLDSYKNGFLNLALPFFGFSEPLAAPRHQYYNQEWTLWDRFEVQGLQPNGEEMTLKQFLDYFKTEHKLEITMLSQGVSMLYSFFMPAAKLKERLDQPMTEIVSRVSKRKLGRHVRALVLELCCNDESGEDVEVPYVRYTIR
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P22314 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| S3 | Phosphorylation | Uniprot | |
| S4 | Phosphorylation | P06493 (CDK1) | Uniprot |
| S7 | Phosphorylation | Uniprot | |
| S13 | Phosphorylation | Uniprot | |
| S21 | Phosphorylation | Uniprot | |
| S24 | Phosphorylation | Uniprot | |
| S28 | Phosphorylation | Uniprot | |
| S31 | Phosphorylation | Uniprot | |
| S46 | Phosphorylation | Uniprot | |
| Y55 | Phosphorylation | Uniprot | |
| S56 | Phosphorylation | Uniprot | |
| Y60 | Phosphorylation | Uniprot | |
| K68 | Acetylation | Uniprot | |
| K68 | Ubiquitination | Uniprot | |
| S74 | Phosphorylation | Uniprot | |
| R81 | Methylation | Uniprot | |
| K89 | Acetylation | Uniprot | |
| K89 | Ubiquitination | Uniprot | |
| K97 | Ubiquitination | Uniprot | |
| R119 | Methylation | Uniprot | |
| S140 | Phosphorylation | Uniprot | |
| K185 | Ubiquitination | Uniprot | |
| T191 | Phosphorylation | Uniprot | |
| C234 | S-Nitrosylation | Uniprot | |
| Y273 | Phosphorylation | Uniprot | |
| T274 | Phosphorylation | Uniprot | |
| S284 | Phosphorylation | Uniprot | |
| Y286 | Phosphorylation | Uniprot | |
| R288 | Methylation | Uniprot | |
| S293 | Phosphorylation | Uniprot | |
| K296 | Ubiquitination | Uniprot | |
| K304 | Methylation | Uniprot | |
| K304 | Ubiquitination | Uniprot | |
| S305 | Phosphorylation | Uniprot | |
| S309 | Phosphorylation | Uniprot | |
| T318 | Phosphorylation | Uniprot | |
| K322 | Ubiquitination | Uniprot | |
| R368 | Methylation | Uniprot | |
| R384 | Methylation | Uniprot | |
| K385 | Ubiquitination | Uniprot | |
| Y388 | Phosphorylation | Uniprot | |
| K411 | Ubiquitination | Uniprot | |
| K416 | Ubiquitination | Uniprot | |
| K443 | Ubiquitination | Uniprot | |
| S460 | Phosphorylation | Uniprot | |
| K465 | Acetylation | Uniprot | |
| K465 | Ubiquitination | Uniprot | |
| K468 | Ubiquitination | Uniprot | |
| K470 | Acetylation | Uniprot | |
| K470 | Ubiquitination | Uniprot | |
| C481 | S-Nitrosylation | Uniprot | |
| K526 | Ubiquitination | Uniprot | |
| K528 | Sumoylation | Uniprot | |
| K528 | Ubiquitination | Uniprot | |
| R551 | Methylation | Uniprot | |
| R558 | Methylation | Uniprot | |
| Y560 | Phosphorylation | Uniprot | |
| C588 | S-Nitrosylation | Uniprot | |
| Y590 | Phosphorylation | Uniprot | |
| K593 | Acetylation | Uniprot | |
| K593 | Ubiquitination | Uniprot | |
| T600 | Phosphorylation | Uniprot | |
| T603 | Phosphorylation | Uniprot | |
| K604 | Acetylation | Uniprot | |
| K604 | Sumoylation | Uniprot | |
| K604 | Ubiquitination | Uniprot | |
| T615 | Phosphorylation | Uniprot | |
| K627 | Acetylation | Uniprot | |
| K627 | Ubiquitination | Uniprot | |
| S628 | Phosphorylation | Uniprot | |
| C632 | S-Nitrosylation | Uniprot | |
| K635 | Sumoylation | Uniprot | |
| K635 | Ubiquitination | Uniprot | |
| K657 | Ubiquitination | Uniprot | |
| K671 | Acetylation | Uniprot | |
| K671 | Ubiquitination | Uniprot | |
| R678 | Methylation | Uniprot | |
| T682 | Phosphorylation | Uniprot | |
| K746 | Ubiquitination | Uniprot | |
| S781 | Phosphorylation | Uniprot | |
| T789 | Phosphorylation | Uniprot | |
| S793 | Phosphorylation | Uniprot | |
| T800 | Phosphorylation | Uniprot | |
| K802 | Ubiquitination | Uniprot | |
| S803 | Phosphorylation | Uniprot | |
| K806 | Ubiquitination | Uniprot | |
| S810 | Phosphorylation | Uniprot | |
| S816 | Phosphorylation | Uniprot | |
| S820 | Phosphorylation | Uniprot | |
| S824 | Phosphorylation | Uniprot | |
| K830 | Ubiquitination | Uniprot | |
| T832 | Phosphorylation | Uniprot | |
| S835 | Phosphorylation | P06493 (CDK1) | Uniprot |
| K838 | Acetylation | Uniprot | |
| K838 | Ubiquitination | Uniprot | |
| K843 | Ubiquitination | Uniprot | |
| S855 | Phosphorylation | Uniprot | |
| S866 | Phosphorylation | Uniprot | |
| Y873 | Phosphorylation | Uniprot | |
| R880 | Methylation | Uniprot | |
| K889 | Ubiquitination | Uniprot | |
| K923 | Ubiquitination | Uniprot | |
| R957 | Methylation | Uniprot | |
| Y978 | Phosphorylation | Uniprot | |
| K980 | Acetylation | Uniprot | |
| K980 | Ubiquitination | Uniprot | |
| K1024 | Acetylation | Uniprot | |
| C1039 | S-Nitrosylation | Uniprot | |
| C1040 | S-Nitrosylation | Uniprot | |
| S1044 | Phosphorylation | Uniprot |
研究背景
Catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin-proteasome system. Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Essential for the formation of radiation-induced foci, timely DNA repair and for response to replication stress. Promotes the recruitment of TP53BP1 and BRCA1 at DNA damage sites.
ISGylated.
Cytoplasm. Mitochondrion. Nucleus.
Nucleus.
Cytoplasm.
Detected in erythrocytes (at protein level). Ubiquitous.
Monomer (By similarity). Interacts with GAN (via BTB domain).
The first 11 amino acids are essential for phosphorylation and exclusive nuclear localization.
Belongs to the ubiquitin-activating E1 family.
研究领域
· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis. (View pathway)
· Human Diseases > Neurodegenerative diseases > Parkinson's disease.
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