Glucose 6 phosphate isomerase Antibody - #DF13660
产品: | Glucose 6 phosphate isomerase 抗体 |
货号: | DF13660 |
描述: | Rabbit polyclonal antibody to Glucose 6 phosphate isomerase |
应用: | WB IHC |
反应: | Human, Mouse, Rat |
预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus |
分子量: | 63kDa; 63kD(Calculated). |
蛋白号: | P06744 |
RRID: | AB_2846679 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF13660, RRID:AB_2846679.
展开/折叠
AMF; Aurocrine motility factor; Autocrine motility factor; DKFZp686C13233; EC 5.3.1.9; G6PI_HUMAN; Glucose phosphate isomerase; Glucose-6-phosphate isomerase; GNPI; GPI; Gpi1; Hexose monophosphate isomerase; Hexosephosphate isomerase; Neuroleukin; NLK; Oxoisomerase; PGI; PHI; Phosphoglucose isomerase; Phosphohexomutase; Phosphohexose isomerase; Phosphosaccharomutase; SA 36; SA-36; SA36; Sperm antigen 36;
抗原和靶标
- P06744 G6PI_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAALTRDPQFQKLQQWYREHRSELNLRRLFDANKDRFNHFSLTLNTNHGHILVDYSKNLVTEDVMRMLVDLAKSRGVEAARERMFNGEKINYTEGRAVLHVALRNRSNTPILVDGKDVMPEVNKVLDKMKSFCQRVRSGDWKGYTGKTITDVINIGIGGSDLGPLMVTEALKPYSSGGPRVWYVSNIDGTHIAKTLAQLNPESSLFIIASKTFTTQETITNAETAKEWFLQAAKDPSAVAKHFVALSTNTTKVKEFGIDPQNMFEFWDWVGGRYSLWSAIGLSIALHVGFDNFEQLLSGAHWMDQHFRTTPLEKNAPVLLALLGIWYINCFGCETHAMLPYDQYLHRFAAYFQQGDMESNGKYITKSGTRVDHQTGPIVWGEPGTNGQHAFYQLIHQGTKMIPCDFLIPVQTQHPIRKGLHHKILLANFLAQTEALMRGKSTEEARKELQAAGKSPEDLERLLPHKVFEGNRPTNSIVFTKLTPFMLGALVAMYEHKIFVQGIIWDINSFDQWGVELGKQLAKKIEPELDGSAQVTSHDASTNGLINFIKQQREARVQ
种属预测
score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P06744 作为底物
Site | PTM Type | Enzyme | Source |
---|---|---|---|
A2 | Acetylation | Uniprot | |
K12 | Acetylation | Uniprot | |
K12 | Methylation | Uniprot | |
K12 | Ubiquitination | Uniprot | |
Y17 | Phosphorylation | Uniprot | |
S22 | Phosphorylation | Uniprot | |
K34 | Acetylation | Uniprot | |
K34 | Ubiquitination | Uniprot | |
Y55 | Phosphorylation | Uniprot | |
S56 | Phosphorylation | Uniprot | |
T61 | Phosphorylation | Uniprot | |
R66 | Methylation | Uniprot | |
K73 | Ubiquitination | Uniprot | |
S74 | Phosphorylation | Uniprot | |
K89 | Acetylation | Uniprot | |
K89 | Ubiquitination | Uniprot | |
Y92 | Phosphorylation | Uniprot | |
T93 | Phosphorylation | Uniprot | |
R106 | Methylation | Uniprot | |
S107 | Phosphorylation | Uniprot | |
T109 | Phosphorylation | Uniprot | |
K116 | Acetylation | Uniprot | |
K116 | Ubiquitination | Uniprot | |
K124 | Acetylation | Uniprot | |
K124 | Ubiquitination | Uniprot | |
K130 | Ubiquitination | Uniprot | |
S131 | Phosphorylation | Uniprot | |
S138 | Phosphorylation | Uniprot | |
K142 | Acetylation | Uniprot | |
K142 | Ubiquitination | Uniprot | |
K147 | Ubiquitination | Uniprot | |
S160 | Phosphorylation | Uniprot | |
T168 | Phosphorylation | Uniprot | |
K172 | Ubiquitination | Uniprot | |
Y174 | Phosphorylation | Uniprot | |
S176 | Phosphorylation | Uniprot | |
Y183 | Phosphorylation | Uniprot | |
S185 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
K194 | Ubiquitination | Uniprot | |
T195 | Phosphorylation | Uniprot | |
S210 | Phosphorylation | Uniprot | |
K211 | Acetylation | Uniprot | |
K211 | Ubiquitination | Uniprot | |
T214 | Phosphorylation | Uniprot | |
T215 | Phosphorylation | Uniprot | |
K226 | Ubiquitination | Uniprot | |
K234 | Acetylation | Uniprot | |
K234 | Ubiquitination | Uniprot | |
S237 | Phosphorylation | Uniprot | |
K241 | Ubiquitination | Uniprot | |
S247 | Phosphorylation | Uniprot | |
T248 | Phosphorylation | Uniprot | |
T250 | Phosphorylation | Uniprot | |
T251 | Phosphorylation | Uniprot | |
K252 | Acetylation | Uniprot | |
K252 | Ubiquitination | Uniprot | |
S359 | Phosphorylation | Uniprot | |
K362 | Acetylation | Uniprot | |
K362 | Ubiquitination | Uniprot | |
K366 | Ubiquitination | Uniprot | |
C404 | S-Nitrosylation | Uniprot | |
T412 | Phosphorylation | Uniprot | |
K423 | Ubiquitination | Uniprot | |
R438 | Methylation | Uniprot | |
S441 | Phosphorylation | Uniprot | |
K447 | Ubiquitination | Uniprot | |
K454 | Acetylation | Uniprot | |
K454 | Ubiquitination | Uniprot | |
S455 | Phosphorylation | Uniprot | |
K466 | Acetylation | Uniprot | |
K466 | Sumoylation | Uniprot | |
K466 | Ubiquitination | Uniprot | |
K524 | Ubiquitination | Uniprot | |
S532 | Phosphorylation | Uniprot |
研究背景
In the cytoplasm, catalyzes the conversion of glucose-6-phosphate to fructose-6-phosphate, the second step in glycolysis, and the reverse reaction during gluconeogenesis. Besides it's role as a glycolytic enzyme, also acts as a secreted cytokine: acts as an angiogenic factor (AMF) that stimulates endothelial cell motility. Acts as a neurotrophic factor, neuroleukin, for spinal and sensory neurons. It is secreted by lectin-stimulated T-cells and induces immunoglobulin secretion.
Phosphorylation at Ser-185 by CK2 has been shown to decrease enzymatic activity and may contribute to secretion by a non-classical secretory pathway.
ISGylated.
Cytoplasm. Secreted.
Homodimer; in the catalytically active form. Monomer in the secreted form.
Belongs to the GPI family.
研究领域
· Metabolism > Carbohydrate metabolism > Glycolysis / Gluconeogenesis.
· Metabolism > Carbohydrate metabolism > Pentose phosphate pathway.
· Metabolism > Carbohydrate metabolism > Starch and sucrose metabolism.
· Metabolism > Carbohydrate metabolism > Amino sugar and nucleotide sugar metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
· Metabolism > Global and overview maps > Carbon metabolism.
文献引用
Application: WB Species: Human Sample: H1299 cells
Application: WB Species: human Sample: H1299 cells
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