产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF13663, RRID:AB_2846682.
展开/折叠
;Branched chain alpha keto acid dehydrogenase E1 component alpha polypeptide; FLJ45695; OVD1A; 2 oxoisovalerate dehydrogenase (lipoamide); 2 oxoisovalerate dehydrogenase subunit alpha, mitochondrial; 2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial; BCKDE1A; BCKDH E1 alpha; BCKDH E1-alpha; BCKDHA; Branched chain alpha keto acid dehydrogenase E1 component alpha chain; Branched chain keto acid dehydrogenase E1 alpha polypeptide; Branched chain keto acid dehydrogenase E1, alpha polypeptide (maple syrup urine disease); Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain; MSU; MSUD1; ODBA_HUMAN;
抗原和靶标
- P12694 ODBA_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAVAIAAARVWRLNRGLSQAALLLLRQPGARGLARSHPPRQQQQFSSLDDKPQFPGASAEFIDKLEFIQPNVISGIPIYRVMDRQGQIINPSEDPHLPKEKVLKLYKSMTLLNTMDRILYESQRQGRISFYMTNYGEEGTHVGSAAALDNTDLVFGQYREAGVLMYRDYPLELFMAQCYGNISDLGKGRQMPVHYGCKERHFVTISSPLATQIPQAVGAAYAAKRANANRVVICYFGEGAASEGDAHAGFNFAATLECPIIFFCRNNGYAISTPTSEQYRGDGIAARGPGYGIMSIRVDGNDVFAVYNATKEARRRAVAENQPFLIEAMTYRIGHHSTSDDSSAYRSVDEVNYWDKQDHPISRLRHYLLSQGWWDEEQEKAWRKQSRRKVMEAFEQAERKPKPNPNLLFSDVYQEMPAQLRKQQESLARHLQTYGEHYPLDHFDK
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P12694 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S46 | Phosphorylation | Uniprot | |
| S47 | Phosphorylation | Uniprot | |
| Y106 | Phosphorylation | Uniprot | |
| K107 | Ubiquitination | Uniprot | |
| T114 | Phosphorylation | Uniprot | |
| R117 | Methylation | Uniprot | |
| S206 | Phosphorylation | Uniprot | |
| S207 | Phosphorylation | Uniprot | |
| K224 | Ubiquitination | Uniprot | |
| S295 | Phosphorylation | Uniprot | |
| Y307 | Phosphorylation | Uniprot | |
| K311 | Ubiquitination | Uniprot | |
| S337 | Phosphorylation | O14874 (BCKDK) | Uniprot |
| T338 | Phosphorylation | Uniprot | |
| S339 | Phosphorylation | Uniprot | |
| S342 | Phosphorylation | Uniprot | |
| S343 | Phosphorylation | Uniprot | |
| Y345 | Phosphorylation | Uniprot | |
| S347 | Phosphorylation | Uniprot | |
| Y353 | Phosphorylation | Uniprot | |
| K356 | Ubiquitination | Uniprot | |
| K445 | Acetylation | Uniprot |
研究背景
The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3).
Mitochondrion matrix.
Heterotetramer of alpha and beta chains.
Belongs to the BCKDHA family.
研究领域
· Metabolism > Amino acid metabolism > Valine, leucine and isoleucine degradation.
· Metabolism > Carbohydrate metabolism > Propanoate metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
限制条款
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