产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF13709, RRID:AB_2846728.
展开/折叠
Cbl interacting protein p70; Cbl interacting protein Sts 1; Cbl interacting protein Sts1; Cbl-interacting protein p70; KIAA1959; MGC15437; Nm23 phosphorylated unknown substrate; p70; SH3 domain containing 70 kDa protein; Sts 1; STS-1; STS1; Suppressor of T cell receptor signaling 1; Suppressor of T-cell receptor signaling 1; T-cell ubiquitin ligand 2; TULA-2; Tyrosine-protein phosphatase STS1/TULA2; Ubash3b; Ubiquitin associated and SH3 domain containing B; Ubiquitin-associated and SH3 domain-containing protein B; UBS3B_HUMAN;
抗原和靶标
- Q8TF42 UBS3B_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAQYGHPSPLGMAAREELYSKVTPRRNRQQRPGTIKHGSALDVLLSMGFPRARAQKALASTGGRSVQAACDWLFSHVGDPFLDDPLPREYVLYLRPTGPLAQKLSDFWQQSKQICGKNKAHNIFPHITLCQFFMCEDSKVDALGEALQTTVSRWKCKFSAPLPLELYTSSNFIGLFVKEDSAEVLKKFAADFAAEAASKTEVHVEPHKKQLHVTLAYHFQASHLPTLEKLAQNIDVKLGCDWVATIFSRDIRFANHETLQVIYPYTPQNDDELELVPGDFIFMSPMEQTSTSEGWIYGTSLTTGCSGLLPENYITKADECSTWIFHGSYSILNTSSSNSLTFGDGVLERRPYEDQGLGETTPLTIICQPMQPLRVNSQPGPQKRCLFVCRHGERMDVVFGKYWLSQCFDAKGRYIRTNLNMPHSLPQRSGGFRDYEKDAPITVFGCMQARLVGEALLESNTIIDHVYCSPSLRCVQTAHNILKGLQQENHLKIRVEPGLFEWTKWVAGSTLPAWIPPSELAAANLSVDTTYRPHIPISKLVVSESYDTYISRSFQVTKEIISECKSKGNNILIVAHASSLEACTCQLQGLSPQNSKDFVQMVRKIPYLGFCSCEELGETGIWQLTDPPILPLTHGPTGGFNWRETLLQE
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q8TF42 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S8 | Phosphorylation | Uniprot | |
| Y19 | Phosphorylation | Uniprot | |
| S20 | Phosphorylation | Uniprot | |
| K21 | Ubiquitination | Uniprot | |
| T23 | Phosphorylation | Uniprot | |
| T34 | Phosphorylation | Uniprot | |
| S46 | Phosphorylation | Uniprot | |
| K56 | Ubiquitination | Uniprot | |
| K112 | Ubiquitination | Uniprot | |
| K157 | Ubiquitination | Uniprot | |
| K187 | Ubiquitination | Uniprot | |
| K208 | Ubiquitination | Uniprot | |
| K237 | Ubiquitination | Uniprot | |
| Y352 | Phosphorylation | Uniprot | |
| S377 | Phosphorylation | Uniprot | |
| K401 | Ubiquitination | Uniprot | |
| Y402 | Phosphorylation | Uniprot | |
| K411 | Ubiquitination | Uniprot | |
| K483 | Ubiquitination | Uniprot | |
| K492 | Ubiquitination | Uniprot | |
| K558 | Ubiquitination | Uniprot | |
| K596 | Ubiquitination | Uniprot |
研究背景
Interferes with CBL-mediated down-regulation and degradation of receptor-type tyrosine kinases. Promotes accumulation of activated target receptors, such as T-cell receptors and EGFR, on the cell surface. Exhibits tyrosine phosphatase activity toward several substrates including EGFR, FAK, SYK, and ZAP70. Down-regulates proteins that are dually modified by both protein tyrosine phosphorylation and ubiquitination.
Cytoplasm. Nucleus.
Homodimer. Interacts with JAK2 (in vitro) (By similarity). Interacts with CBL. Part of a complex containing CBL and activated EGFR. Interacts with ubiquitin and with mono-ubiquitinated proteins. Interacts with ZAP70 (ubiquitinated form).
限制条款
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