| 产品: | Histone H1.1 抗体 |
| 货号: | DF13743 |
| 描述: | Rabbit polyclonal antibody to Histone H1.1 |
| 应用: | WB IF/ICC |
| 反应: | Human |
| 预测: | Horse |
| 分子量: | 21kDa; 22kD(Calculated). |
| 蛋白号: | Q02539 |
| RRID: | AB_2846762 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF13743, RRID:AB_2846762.
展开/折叠
H1 histone family member 1; H1.1; H1F1; HIST1; HIST1H1A; Histone 1 H1a; Histone cluster 1 H1a;
抗原和靶标
- Q02539 H11_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSETVPPAPAASAAPEKPLAGKKAKKPAKAAAASKKKPAGPSVSELIVQAASSSKERGGVSLAALKKALAAAGYDVEKNNSRIKLGIKSLVSKGTLVQTKGTGASGSFKLNKKASSVETKPGASKVATKTKATGASKKLKKATGASKKSVKTPKKAKKPAATRKSSKNPKKPKTVKPKKVAKSPAKAKAVKPKAAKARVTKPKTAKPKKAAPKKK
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q02539 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Phosphorylation | Uniprot | |
| T4 | Phosphorylation | P06493 (CDK1) | Uniprot |
| K17 | Ubiquitination | Uniprot | |
| K26 | Acetylation | Uniprot | |
| K55 | Ubiquitination | Uniprot | |
| S61 | Phosphorylation | Uniprot | |
| K67 | Sumoylation | Uniprot | |
| K67 | Ubiquitination | Uniprot | |
| Y74 | Phosphorylation | Uniprot | |
| K78 | Acetylation | Uniprot | |
| K78 | Ubiquitination | Uniprot | |
| S81 | Phosphorylation | Uniprot | |
| K88 | Acetylation | Uniprot | |
| K88 | Ubiquitination | Uniprot | |
| S89 | Phosphorylation | Uniprot | |
| S92 | Phosphorylation | Uniprot | |
| K93 | Acetylation | Uniprot | |
| K93 | Ubiquitination | Uniprot | |
| T95 | Phosphorylation | Uniprot | |
| K100 | Ubiquitination | Uniprot | |
| T102 | Phosphorylation | Uniprot | |
| S105 | Phosphorylation | Uniprot | |
| S107 | Phosphorylation | Uniprot | |
| K109 | Acetylation | Uniprot | |
| K109 | Ubiquitination | Uniprot | |
| K112 | Ubiquitination | Uniprot | |
| K120 | Ubiquitination | Uniprot | |
| K125 | Ubiquitination | Uniprot | |
| T152 | Phosphorylation | P24941 (CDK2) | Uniprot |
| T162 | Phosphorylation | Uniprot | |
| S165 | Phosphorylation | Uniprot | |
| S166 | Phosphorylation | Uniprot | |
| S183 | Phosphorylation | Q15369 (ELOC) , P50750 (CDK9) | Uniprot |
| T204 | Phosphorylation | Uniprot |
研究背景
Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).
H1 histones are progressively phosphorylated during the cell cycle, becoming maximally phosphorylated during late G2 phase and M phase, and being dephosphorylated sharply thereafter.
Citrullination at Arg-57 (H1R54ci) by PADI4 takes place within the DNA-binding site of H1 and results in its displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance.
Nucleus. Chromosome.
Note: Mainly localizes in euchromatin.
Interacts with DFFB.
The C-terminal domain is required for high-affinity binding to chromatin.
Belongs to the histone H1/H5 family.
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