| 产品: | AS160 抗体 |
| 货号: | AF2618 |
| 描述: | Rabbit polyclonal antibody to AS160 |
| 应用: | WB |
| 反应: | Human, Mouse, Rat |
| 预测: | Bovine, Horse, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 160kDa; 147kD(Calculated). |
| 蛋白号: | O60343 |
| RRID: | AB_2846786 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF2618, RRID:AB_2846786.
展开/折叠
Acrg embryonic lethality (mouse) minimal region ortholog; Acrg embryonic lethality minimal region ortholog; Acrg embryonic lethality mouse minimal region ortholog; Akt substrate of 160 kDa; AS 160; AS160; BUB2; CDC16; KIAA0603; NIDDM5; TBC (Tre 2 BUB2 CDC16) domain containing protein; TBC Tre 2 BUB2 CDC16 domain containing protein; TBC1 D4; TBC1 domain family member 4; Tbc1d4; TBCD4_HUMAN; Tre-2;
抗原和靶标
A synthesized peptide derived from human AS160.
Widely expressed. Isoform 2 is the highest overexpressed in most tissues. Isoform 1 is highly expressed in skeletal muscle and heart, but was not detectable in the liver nor in adipose tissue. Isoform 2 is strongly expressed in adrenal and thyroid gland, and also in lung, kidney, colon, brain and adipose tissue. Isoform 2 is moderately expressed in skeletal muscle. Expressed in pancreatic Langerhans islets, including beta cells (at protein level). Expression is decreased by twofold in pancreatic islets in type 2 diabetes patients compared to control subjects. Up-regulated in T-cells from patients with atopic dermatitis.
- O60343 TBCD4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEPPSCIQDEPFPHPLEPEPGVSAQPGPGKPSDKRFRLWYVGGSCLDHRTTLPMLPWLMAEIRRRSQKPEAGGCGAPAAREVILVLSAPFLRCVPAPGAGASGGTSPSATQPNPAVFIFEHKAQHISRFIHNSHDLTYFAYLIKAQPDDPESQMACHVFRATDPSQVPDVISSIRQLSKAAMKEDAKPSKDNEDAFYNSQKFEVLYCGKVTVTHKKAPSSLIDDCMEKFSLHEQQRLKIQGEQRGPDPGEDLADLEVVVPGSPGDCLPEEADGTDTHLGLPAGASQPALTSSRVCFPERILEDSGFDEQQEFRSRCSSVTGVQRRVHEGSQKSQPRRRHASAPSHVQPSDSEKNRTMLFQVGRFEINLISPDTKSVVLEKNFKDISSCSQGIKHVDHFGFICRESPEPGLSQYICYVFQCASESLVDEVMLTLKQAFSTAAALQSAKTQIKLCEACPMHSLHKLCERIEGLYPPRAKLVIQRHLSSLTDNEQADIFERVQKMKPVSDQEENELVILHLRQLCEAKQKTHVHIGEGPSTISNSTIPENATSSGRFKLDILKNKAKRSLTSSLENIFSRGANRMRGRLGSVDSFERSNSLASEKDYSPGDSPPGTPPASPPSSAWQTFPEEDSDSPQFRRRAHTFSHPPSSTKRKLNLQDGRAQGVRSPLLRQSSSEQCSNLSSVRRMYKESNSSSSLPSLHTSFSAPSFTAPSFLKSFYQNSGRLSPQYENEIRQDTASESSDGEGRKRTSSTCSNESLSVGGTSVTPRRISWRQRIFLRVASPMNKSPSAMQQQDGLDRNELLPLSPLSPTMEEEPLVVFLSGEDDPEKIEERKKSKELRSLWRKAIHQQILLLRMEKENQKLEASRDELQSRKVKLDYEEVGACQKEVLITWDKKLLNCRAKIRCDMEDIHTLLKEGVPKSRRGEIWQFLALQYRLRHRLPNKQQPPDISYKELLKQLTAQQHAILVDLGRTFPTHPYFSVQLGPGQLSLFNLLKAYSLLDKEVGYCQGISFVAGVLLLHMSEEQAFEMLKFLMYDLGFRKQYRPDMMSLQIQMYQLSRLLHDYHRDLYNHLEENEISPSLYAAPWFLTLFASQFSLGFVARVFDIIFLQGTEVIFKVALSLLSSQETLIMECESFENIVEFLKNTLPDMNTSEMEKIITQVFEMDISKQLHAYEVEYHVLQDELQESSYSCEDSETLEKLERANSQLKRQNMDLLEKLQVAHTKIQALESNLENLLTRETKMKSLIRTLEQEKMAYQKTVEQLRKLLPADALVNCDLLLRDLNCNPNNKAKIGNKP
翻译修饰 - O60343 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| S44 | Phosphorylation | Uniprot | |
| S66 | Phosphorylation | Uniprot | |
| K68 | Ubiquitination | Uniprot | |
| S102 | Phosphorylation | Uniprot | |
| K228 | Ubiquitination | Uniprot | |
| K238 | Ubiquitination | Uniprot | |
| S262 | Phosphorylation | Uniprot | |
| T274 | Phosphorylation | Uniprot | |
| S285 | Phosphorylation | Uniprot | |
| S304 | Phosphorylation | Uniprot | |
| S314 | Phosphorylation | Uniprot | |
| S317 | Phosphorylation | Uniprot | |
| S318 | Phosphorylation | O00141 (SGK1) , P31749 (AKT1) | Uniprot |
| T320 | Phosphorylation | Uniprot | |
| S330 | Phosphorylation | Uniprot | |
| S341 | Phosphorylation | Q15418 (RPS6KA1) | Uniprot |
| S344 | Phosphorylation | Uniprot | |
| S349 | Phosphorylation | Uniprot | |
| S351 | Phosphorylation | Uniprot | |
| T356 | Phosphorylation | Uniprot | |
| S370 | Phosphorylation | Uniprot | |
| K383 | Ubiquitination | Uniprot | |
| K477 | Acetylation | Uniprot | |
| S485 | Phosphorylation | O14757 (CHEK1) | Uniprot |
| S486 | Phosphorylation | Uniprot | |
| T488 | Phosphorylation | Uniprot | |
| S551 | Phosphorylation | Uniprot | |
| K555 | Ubiquitination | Uniprot | |
| K560 | Ubiquitination | Uniprot | |
| S566 | Phosphorylation | Uniprot | |
| T568 | Phosphorylation | O00141 (SGK1) | Uniprot |
| S569 | Phosphorylation | Uniprot | |
| S570 | Phosphorylation | Uniprot | |
| S576 | Phosphorylation | Uniprot | |
| S588 | Phosphorylation | O14757 (CHEK1) , P31749 (AKT1) , O00141 (SGK1) | Uniprot |
| S591 | Phosphorylation | Uniprot | |
| S595 | Phosphorylation | Uniprot | |
| S597 | Phosphorylation | Uniprot | |
| S600 | Phosphorylation | Uniprot | |
| S609 | Phosphorylation | Uniprot | |
| T613 | Phosphorylation | Uniprot | |
| S617 | Phosphorylation | Uniprot | |
| S621 | Phosphorylation | Uniprot | |
| S633 | Phosphorylation | Uniprot | |
| T642 | Phosphorylation | O00141 (SGK1) , P31749 (AKT1) | Uniprot |
| S644 | Phosphorylation | Uniprot | |
| S648 | Phosphorylation | Uniprot | |
| S649 | Phosphorylation | Uniprot | |
| S666 | Phosphorylation | Uniprot | |
| S672 | Phosphorylation | Uniprot | |
| S673 | Phosphorylation | Uniprot | |
| S678 | Phosphorylation | Uniprot | |
| T701 | Phosphorylation | Uniprot | |
| S704 | Phosphorylation | Uniprot | |
| S707 | Phosphorylation | Uniprot | |
| T749 | Phosphorylation | Uniprot | |
| S750 | Phosphorylation | Uniprot | |
| S751 | Phosphorylation | O00141 (SGK1) , Q15418 (RPS6KA1) | Uniprot |
| T752 | Phosphorylation | Uniprot | |
| S754 | Phosphorylation | Uniprot | |
| S757 | Phosphorylation | Uniprot | |
| S759 | Phosphorylation | Uniprot | |
| T763 | Phosphorylation | Uniprot | |
| S764 | Phosphorylation | Uniprot | |
| T766 | Phosphorylation | Uniprot | |
| S782 | Phosphorylation | Uniprot | |
| S787 | Phosphorylation | Uniprot | |
| S789 | Phosphorylation | Uniprot | |
| S806 | Phosphorylation | Uniprot | |
| S809 | Phosphorylation | Uniprot | |
| Y1191 | Phosphorylation | Uniprot | |
| S1207 | Phosphorylation | Uniprot | |
| S1246 | Phosphorylation | Uniprot | |
| K1255 | Ubiquitination | Uniprot | |
| K1267 | Ubiquitination | Uniprot |
研究背景
May act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake.
Phosphorylated by AKT1; insulin-induced. Also phosphorylated by AMPK in response to insulin. Insulin-stimulated phosphorylation is required for SLC2A4/GLUT4 translocation. Has no effect on SLC2A4/GLUT4 internalization. Physiological hyperinsulinemia increases phosphorylation in skeletal muscle. Insulin-stimulated phosphorylation is reduced by 39% in type 2 diabetic patients.
Cytoplasm.
Note: Isoform 2 shows a cytoplasmic perinuclear localization in a myoblastic cell line in resting and insulin-stimulated cells.
Widely expressed. Isoform 2 is the highest overexpressed in most tissues. Isoform 1 is highly expressed in skeletal muscle and heart, but was not detectable in the liver nor in adipose tissue. Isoform 2 is strongly expressed in adrenal and thyroid gland, and also in lung, kidney, colon, brain and adipose tissue. Isoform 2 is moderately expressed in skeletal muscle. Expressed in pancreatic Langerhans islets, including beta cells (at protein level). Expression is decreased by twofold in pancreatic islets in type 2 diabetes patients compared to control subjects. Up-regulated in T-cells from patients with atopic dermatitis.
研究领域
· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.
· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway. (View pathway)
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