Acetyl-SOD2/MnSOD (Lys68) Antibody - #AF3751

Fig. 4 The expression of NAD+, Sirt3, and acetylated SOD2 and microglial inflammation. A NAD+ levels in the hippocampus at PND40 (CON, n = 8; MS, n = 8). B–D Immunoblots and quantification of Sirt3, acetylated SOD2, and total SOD2 in the hippocampus at PND40 (CON, n = 4; MS, n = 4). (E) NAD+ levels in the hippocampus at PND65 (CON, n = 8; MS, n = 8). F–H Immunoblots and quantification of Sirt3, acetylated SOD2, and total SOD2 in the hippocampus at PND65 (CON, n = 6; MS, n = 6). I Representative immunofluorescence images show the expression of Iba1 (green pixels) and Sirt3 (red pixels) in the DG at PND40 (n = 4 per group). J Quantitative analyses of the percentage of Sirt3 expression. (K) Quantitative analyses of the percentage of Iba1 and Sirt3 co-labeling. L Quantitative analyses of Iba1+ immunostained cells. M Representative immunofluorescence images showing the expression of Iba1 (green pixels) and Sirt3 (red pixels) in the DG at PND65 (n = 4 per group). N Quantitative analyses of the percentage of Sirt3 expression. O Quantitative analyses of the percentage of Iba1 and Sirt3 co-labeling. (P) Quantitative analyses of Iba1+ immunostained cells. Q Real-time qPCR analysis of TNF-α, IL-1β, and IL-6 in the hippocampus at PND40 (CON, n = 4; MS, n = 4). R Real-time qPCR analysis of TNF-α, IL-1β, and IL-6 in the hippocampus at PND65 (CON, n = 6; MS, n = 6). Data are presented as mean ± SEM for each group. n.s. not significant;

Fig. 5 SIRT3 inhibition causes mitochondrial damage and increases apoptosis in HT22 cells. A–C Immunoblots and quantification analysis of the level of acetylated SOD2 in HT22 cells (n = 3, per group). Data were normalized to controls. D Representative images of ROS (green) levels in HT22 cells. E Quantitative analyses of the percentage ROS area the HT22 cells (n = 6, per group). F Representative images of the mitochondrial membrane potential in HT22 cells. G Bar graph presenting the green/red fluorescence ratio, which reflects changes in the mitochondrial membrane potential. H Neuronal apoptosis was assessed by TUNEL staining in HT22 cells. TUNEL positive cells (red), DAPI (blue) labeling. I Quantification of TUNEL‐positive cells in HT22 cells (n = 4 per group). J Apoptotic HT22 cells were detected by flow cytometry. K The percentage of apoptotic cells was determined (n = 4, per group). L, M Immunoblots and quantification analysis of cleaved caspase 3 levels in HT22 cells (n = 4, per group). Data were normalized to controls. The data are presented as mean ± SEM for each group. n.s. was not significant;