产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF6128, RRID:AB_2835011.
展开/折叠
AUTS9; c met; D249; Hepatocyte growth factor receptor; HGF; HGF receptor; HGF/SF receptor; HGFR; MET; Met proto oncogene tyrosine kinase; MET proto oncogene, receptor tyrosine kinase; Met proto-oncogene (hepatocyte growth factor receptor); Met proto-oncogene; Met protooncogene; MET_HUMAN; Oncogene MET; Par4; Proto-oncogene c-Met; RCCP2; Scatter factor receptor; SF receptor; Tyrosine-protein kinase Met;
抗原和靶标
Expressed in normal hepatocytes as well as in epithelial cells lining the stomach, the small and the large intestine. Found also in basal keratinocytes of esophagus and skin. High levels are found in liver, gastrointestinal tract, thyroid and kidney. Also present in the brain. Expressed in metaphyseal bone (at protein level) (PubMed:26637977).
- P08581 MET_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKAPAVLAPGILVLLFTLVQRSNGECKEALAKSEMNVNMKYQLPNFTAETPIQNVILHEHHIFLGATNYIYVLNEEDLQKVAEYKTGPVLEHPDCFPCQDCSSKANLSGGVWKDNINMALVVDTYYDDQLISCGSVNRGTCQRHVFPHNHTADIQSEVHCIFSPQIEEPSQCPDCVVSALGAKVLSSVKDRFINFFVGNTINSSYFPDHPLHSISVRRLKETKDGFMFLTDQSYIDVLPEFRDSYPIKYVHAFESNNFIYFLTVQRETLDAQTFHTRIIRFCSINSGLHSYMEMPLECILTEKRKKRSTKKEVFNILQAAYVSKPGAQLARQIGASLNDDILFGVFAQSKPDSAEPMDRSAMCAFPIKYVNDFFNKIVNKNNVRCLQHFYGPNHEHCFNRTLLRNSSGCEARRDEYRTEFTTALQRVDLFMGQFSEVLLTSISTFIKGDLTIANLGTSEGRFMQVVVSRSGPSTPHVNFLLDSHPVSPEVIVEHTLNQNGYTLVITGKKITKIPLNGLGCRHFQSCSQCLSAPPFVQCGWCHDKCVRSEECLSGTWTQQICLPAIYKVFPNSAPLEGGTRLTICGWDFGFRRNNKFDLKKTRVLLGNESCTLTLSESTMNTLKCTVGPAMNKHFNMSIIISNGHGTTQYSTFSYVDPVITSISPKYGPMAGGTLLTLTGNYLNSGNSRHISIGGKTCTLKSVSNSILECYTPAQTISTEFAVKLKIDLANRETSIFSYREDPIVYEIHPTKSFISGGSTITGVGKNLNSVSVPRMVINVHEAGRNFTVACQHRSNSEIICCTTPSLQQLNLQLPLKTKAFFMLDGILSKYFDLIYVHNPVFKPFEKPVMISMGNENVLEIKGNDIDPEAVKGEVLKVGNKSCENIHLHSEAVLCTVPNDLLKLNSELNIEWKQAISSTVLGKVIVQPDQNFTGLIAGVVSISTALLLLLGFFLWLKKRKQIKDLGSELVRYDARVHTPHLDRLVSARSVSPTTEMVSNESVDYRATFPEDQFPNSSQNGSCRQVQYPLTDMSPILTSGDSDISSPLLQNTVHIDLSALNPELVQAVQHVVIGPSSLIVHFNEVIGRGHFGCVYHGTLLDNDGKKIHCAVKSLNRITDIGEVSQFLTEGIIMKDFSHPNVLSLLGICLRSEGSPLVVLPYMKHGDLRNFIRNETHNPTVKDLIGFGLQVAKGMKYLASKKFVHRDLAARNCMLDEKFTVKVADFGLARDMYDKEYYSVHNKTGAKLPVKWMALESLQTQKFTTKSDVWSFGVLLWELMTRGAPPYPDVNTFDITVYLLQGRRLLQPEYCPDPLYEVMLKCWHPKAEMRPSFSELVSRISAIFSTFIGEHYVHVNATYVNVKCVAPYPSLLSSEDNADDEVDTRPASFWETS
种属预测
score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P08581 作为底物
Site | PTM Type | Enzyme | Source |
---|---|---|---|
T17 | Phosphorylation | Uniprot | |
K85 | Ubiquitination | Uniprot | |
N106 | N-Glycosylation | Uniprot | |
S178 | Phosphorylation | Uniprot | |
K189 | Ubiquitination | Uniprot | |
K223 | Ubiquitination | Uniprot | |
K324 | Ubiquitination | Uniprot | |
K376 | Ubiquitination | Uniprot | |
S468 | Phosphorylation | Uniprot | |
S473 | Phosphorylation | Uniprot | |
S637 | Phosphorylation | Uniprot | |
S641 | Phosphorylation | Uniprot | |
K725 | Ubiquitination | Uniprot | |
Y745 | Phosphorylation | Uniprot | |
T750 | Phosphorylation | Uniprot | |
K751 | Ubiquitination | Uniprot | |
S755 | Phosphorylation | Uniprot | |
S758 | Phosphorylation | Uniprot | |
T759 | Phosphorylation | Uniprot | |
K765 | Ubiquitination | Uniprot | |
Y830 | Phosphorylation | Uniprot | |
K876 | Ubiquitination | Uniprot | |
K962 | Ubiquitination | Uniprot | |
S966 | Phosphorylation | Uniprot | |
Y971 | Phosphorylation | Uniprot | |
T977 | Phosphorylation | Uniprot | |
S985 | Phosphorylation | Q02156 (PRKCE) , P17252 (PRKCA) , Q05655 (PRKCD) | Uniprot |
S988 | Phosphorylation | Uniprot | |
S990 | Phosphorylation | Uniprot | |
T992 | Phosphorylation | Uniprot | |
T993 | Phosphorylation | Uniprot | |
S997 | Phosphorylation | Uniprot | |
S1000 | Phosphorylation | Uniprot | |
Y1003 | Phosphorylation | Uniprot | |
T1006 | Phosphorylation | Uniprot | |
S1016 | Phosphorylation | Uniprot | |
S1020 | Phosphorylation | Uniprot | |
Y1026 | Phosphorylation | Uniprot | |
Y1093 | Phosphorylation | Uniprot | |
T1096 | Phosphorylation | Uniprot | |
K1103 | Ubiquitination | Uniprot | |
K1104 | Ubiquitination | Uniprot | |
K1110 | Ubiquitination | Uniprot | |
Y1159 | Phosphorylation | Uniprot | |
K1161 | Ubiquitination | Uniprot | |
K1179 | Ubiquitination | Uniprot | |
K1190 | Acetylation | Uniprot | |
K1190 | Ubiquitination | Uniprot | |
K1193 | Ubiquitination | Uniprot | |
Y1194 | Phosphorylation | Uniprot | |
K1199 | Ubiquitination | Uniprot | |
K1215 | Ubiquitination | Uniprot | |
K1219 | Ubiquitination | Uniprot | |
Y1230 | Phosphorylation | P08581 (MET) | Uniprot |
K1232 | Ubiquitination | Uniprot | |
Y1234 | Phosphorylation | P08581 (MET) , Q04912 (MST1R) | Uniprot |
Y1235 | Phosphorylation | P08581 (MET) , P51813 (BMX) , Q04912 (MST1R) | Uniprot |
S1236 | Phosphorylation | Uniprot | |
K1240 | Ubiquitination | Uniprot | |
T1241 | Phosphorylation | Uniprot | |
K1244 | Ubiquitination | Uniprot | |
K1248 | Ubiquitination | Uniprot | |
K1259 | Ubiquitination | Uniprot | |
T1289 | Phosphorylation | Uniprot | |
Y1307 | Phosphorylation | Uniprot | |
Y1313 | Phosphorylation | Uniprot | |
K1318 | Ubiquitination | Uniprot | |
S1342 | Phosphorylation | Uniprot | |
T1343 | Phosphorylation | Uniprot | |
Y1349 | Phosphorylation | P00519 (ABL1) , Q04912 (MST1R) , P08581 (MET) | Uniprot |
T1355 | Phosphorylation | Uniprot | |
Y1356 | Phosphorylation | P00519 (ABL1) , Q04912 (MST1R) , P08581 (MET) | Uniprot |
Y1365 | Phosphorylation | P08581 (MET) | Uniprot |
S1367 | Phosphorylation | Uniprot | |
S1370 | Phosphorylation | Uniprot | |
S1371 | Phosphorylation | Uniprot |
翻译修饰 - P08581 作为激酶
Substrate | Site | Source |
---|---|---|
P08581-1 (MET) | T977 | Uniprot |
P08581-1 (MET) | S988 | Uniprot |
P08581-1 (MET) | S990 | Uniprot |
P08581 (MET) | Y1230 | Uniprot |
P08581 (MET) | Y1234 | Uniprot |
P08581 (MET) | Y1235 | Uniprot |
P08581 (MET) | Y1349 | Uniprot |
P08581 (MET) | Y1356 | Uniprot |
P08581 (MET) | Y1365 | Uniprot |
P09874 (PARP1) | Y907 | Uniprot |
P29353 (SHC1) | Y427 | Uniprot |
Q04912 (MST1R) | Y1238 | Uniprot |
Q04912 (MST1R) | Y1239 | Uniprot |
Q04912 (MST1R) | Y1353 | Uniprot |
Q04912 (MST1R) | Y1360 | Uniprot |
Q05397 (PTK2) | Y5 | Uniprot |
Q05397 (PTK2) | Y194 | Uniprot |
Q05397 (PTK2) | Y397 | Uniprot |
Q05397 (PTK2) | Y407 | Uniprot |
Q05397 (PTK2) | Y576 | Uniprot |
Q05397 (PTK2) | Y577 | Uniprot |
Q05397 (PTK2) | Y861 | Uniprot |
Q05397 (PTK2) | Y925 | Uniprot |
Q13480-1 (GAB1) | Y285 | Uniprot |
Q13480 (GAB1) | Y307 | Uniprot |
Q13480 (GAB1) | Y373 | Uniprot |
Q13480-1 (GAB1) | Y406 | Uniprot |
Q13480-2 (GAB1) | Y447 | Uniprot |
Q13480 (GAB1) | Y472 | Uniprot |
Q13480 (GAB1) | Y589 | Uniprot |
Q13480-2 (GAB1) | Y619 | Uniprot |
Q13480-1 (GAB1) | Y627 | Uniprot |
Q13480-2 (GAB1) | Y657 | Uniprot |
Q13480-1 (GAB1) | Y659 | Uniprot |
Q13480-2 (GAB1) | Y689 | Uniprot |
研究背景
Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. The RAS-ERK activation is associated with the morphogenetic effects while PI3K/AKT coordinates prosurvival effects. During embryonic development, MET signaling plays a role in gastrulation, development and migration of muscles and neuronal precursors, angiogenesis and kidney formation. In adults, participates in wound healing as well as organ regeneration and tissue remodeling. Promotes also differentiation and proliferation of hematopoietic cells. May regulate cortical bone osteogenesis (By similarity).
(Microbial infection) Acts as a receptor for Listeria monocytogenes internalin InlB, mediating entry of the pathogen into cells.
Autophosphorylated in response to ligand binding on Tyr-1234 and Tyr-1235 in the kinase domain leading to further phosphorylation of Tyr-1349 and Tyr-1356 in the C-terminal multifunctional docking site. Dephosphorylated by PTPRJ at Tyr-1349 and Tyr-1365. Dephosphorylated by PTPN1 and PTPN2.
Ubiquitinated. Ubiquitination by CBL regulates MET endocytosis, resulting in decreasing plasma membrane receptor abundance, and in endosomal degradation and/or recycling of internalized receptors.
(Microbial infection) Tyrosine phosphorylation is stimulated by L.monocytogenes InlB. Tyrosine phosphorylation is maximal 10-20 minutes after treatment with InlB and disappears by 60 minutes. The phosphorylated residues were not identified.
Membrane>Single-pass type I membrane protein.
Secreted.
Expressed in normal hepatocytes as well as in epithelial cells lining the stomach, the small and the large intestine. Found also in basal keratinocytes of esophagus and skin. High levels are found in liver, gastrointestinal tract, thyroid and kidney. Also present in the brain. Expressed in metaphyseal bone (at protein level).
Heterodimer made of an alpha chain (50 kDa) and a beta chain (145 kDa) which are disulfide linked. Binds PLXNB1. Interacts when phosphorylated with downstream effectors including STAT3, PIK3R1, SRC, PCLG1, GRB2 and GAB1. Interacts with SPSB1, SPSB2 and SPSB4 (By similarity). Interacts with INPP5D/SHIP1. When phosphorylated at Tyr-1356, interacts with INPPL1/SHIP2. Interacts with RANBP9 and RANBP10, as well as SPSB1, SPSB2, SPSB3 and SPSB4. SPSB1 binding occurs in the presence and in the absence of HGF, however HGF treatment has a positive effect on this interaction. Interacts with MUC20; prevents interaction with GRB2 and suppresses hepatocyte growth factor-induced cell proliferation. Interacts with GRB10. Interacts with PTPN1 and PTPN2. Interacts with LECT2; this interaction may have an antagonistic effect on receptor activation. Interacts with HSP90AA1 and HSP90AB1; the interaction suppresses MET kinase activity.
(Microbial infection) Interacts via extracytoplasmic residues 25-656 with L.monocytogenes InlB; MET can bind HGF, its endogenous ligand, and InlB simultaneously. InlB probably dimerizes upon binding to MET, which encourages subsequent dimerization of MET (Probable).
The kinase domain is involved in SPSB1 binding.
The beta-propeller Sema domain mediates binding to HGF.
Belongs to the protein kinase superfamily. Tyr protein kinase family.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)
· Cellular Processes > Cellular community - eukaryotes > Adherens junction. (View pathway)
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Ras signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Rap1 signaling pathway. (View pathway)
· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction. (View pathway)
· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)
· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.
· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.
· Human Diseases > Infectious diseases: Bacterial > Epithelial cell signaling in Helicobacter pylori infection.
· Human Diseases > Infectious diseases: Parasitic > Malaria.
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.
· Human Diseases > Cancers: Overview > Proteoglycans in cancer.
· Human Diseases > Cancers: Overview > MicroRNAs in cancer.
· Human Diseases > Cancers: Specific types > Renal cell carcinoma. (View pathway)
· Human Diseases > Cancers: Specific types > Melanoma. (View pathway)
· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma. (View pathway)
· Human Diseases > Cancers: Specific types > Gastric cancer. (View pathway)
· Human Diseases > Cancers: Overview > Central carbon metabolism in cancer. (View pathway)
· Organismal Systems > Development > Axon guidance. (View pathway)
文献引用
Application: WB Species: human Sample: MKN-45 and MKN-28 cells
Application: WB Species: Human Sample: uterine tissues
Application: WB Species: Human Sample: gastric cancer cell
Application: WB Species: human Sample: MKN‑45 cells
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