产品: ATG4B 抗体
货号: AF6505
描述: Rabbit polyclonal antibody to ATG4B
应用: ELISA(peptide)
反应: Human, Mouse
分子量: 40~50kDa; 44kD(Calculated).
蛋白号: Q9Y4P1
RRID: AB_2847229

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 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
克隆:
Polyclonal
特异性:
ATG4B Antibody detects endogenous levels of total ATG4B.
RRID:
AB_2847229
引用格式: Affinity Biosciences Cat# AF6505, RRID:AB_2847229.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

APG4 autophagy 4 homolog B; APG4B; ATG4 autophagy related 4 homolog B (S. cerevisiae); ATG4B; ATG4B_HUMAN; AUT like 1 cysteine endopeptidase; AUT-like 1 cysteine endopeptidase; AUTL1; Autophagin 1; Autophagin-1; Autophagy related 4B cysteine peptidase; Autophagy related cysteine endopeptidase 1; Autophagy related protein 4 homolog B; Autophagy-related cysteine endopeptidase 1; Autophagy-related protein 4 homolog B; Cysteine protease ATG4B; hAPG4B; MGC1353;

抗原和靶标

免疫原:

A synthesized peptide derived from human ATG4B.

Uniprot:
基因/基因ID:
表达:
Q9Y4P1 ATG4B_HUMAN:

Mainly expressed in the skeletal muscle, followed by brain, heart, liver and pancreas.

序列:
MDAATLTYDTLRFAEFEDFPETSEPVWILGRKYSIFTEKDEILSDVASRLWFTYRKNFPAIGGTGPTSDTGWGCMLRCGQMIFAQALVCRHLGRDWRWTQRKRQPDSYFSVLNAFIDRKDSYYSIHQIAQMGVGEGKSIGQWYGPNTVAQVLKKLAVFDTWSSLAVHIAMDNTVVMEEIRRLCRTSVPCAGATAFPADSDRHCNGFPAGAEVTNRPSPWRPLVLLIPLRLGLTDINEAYVETLKHCFMMPQSLGVIGGKPNSAHYFIGYVGEELIYLDPHTTQPAVEPTDGCFIPDESFHCQHPPCRMSIAELDPSIAVGFFCKTEDDFNDWCQQVKKLSLLGGALPMFELVELQPSHLACPDVLNLSLDSSDVERLERFFDSEDEDFEILSL

翻译修饰 - Q9Y4P1 作为底物

Site PTM Type Enzyme
M1 Acetylation
Y8 Phosphorylation
S34 Phosphorylation
K39 Ubiquitination
S44 Phosphorylation
K102 Ubiquitination
K119 Ubiquitination
Y122 Phosphorylation
Y123 Phosphorylation
K153 Ubiquitination
S316 Phosphorylation
K337 Ubiquitination
S383 Phosphorylation
S392 Phosphorylation

研究背景

功能:

Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms.

细胞定位:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Mainly expressed in the skeletal muscle, followed by brain, heart, liver and pancreas.

蛋白家族:

Belongs to the peptidase C54 family.

研究领域

· Cellular Processes > Transport and catabolism > Autophagy - other.   (View pathway)

· Cellular Processes > Transport and catabolism > Autophagy - animal.   (View pathway)

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