| 产品: | hFANCI 抗体 |
| 货号: | AF6527 |
| 描述: | Rabbit polyclonal antibody to hFANCI |
| 应用: | ELISA(peptide) |
| 反应: | Human, Mouse |
| 分子量: | 149kD(Calculated). |
| 蛋白号: | Q9NVI1 |
| RRID: | AB_2847251 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF6527, RRID:AB_2847251.
展开/折叠
FANCI; FANCI gene; FANCI_HUMAN; Fanconi anemia group I protein; Fanconi anemia, complementation group I; FLJ10719; FLJ14658; KIAA1794; Protein FACI; Protein FANCI;
抗原和靶标
A synthesized peptide derived from human hFANCI.
- Q9NVI1 FANCI_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDQKILSLAAEKTADKLQEFLQTLREGDLTNLLQNQAVKGKVAGALLRAIFKGSPCSEEAGTLRRRKIYTCCIQLVESGDLQKEIASEIIGLLMLEAHHFPGPLLVELANEFISAVREGSLVNGKSLELLPIILTALATKKENLAYGKGVLSGEECKKQLINTLCSGRWDQQYVIQLTSMFKDVPLTAEEVEFVVEKALSMFSKMNLQEIPPLVYQLLVLSSKGSRKSVLEGIIAFFSALDKQHNEEQSGDELLDVVTVPSGELRHVEGTIILHIVFAIKLDYELGRELVKHLKVGQQGDSNNNLSPFSIALLLSVTRIQRFQDQVLDLLKTSVVKSFKDLQLLQGSKFLQNLVPHRSYVSTMILEVVKNSVHSWDHVTQGLVELGFILMDSYGPKKVLDGKTIETSPSLSRMPNQHACKLGANILLETFKIHEMIRQEILEQVLNRVVTRASSPISHFLDLLSNIVMYAPLVLQSCSSKVTEAFDYLSFLPLQTVQRLLKAVQPLLKVSMSMRDCLILVLRKAMFANQLDARKSAVAGFLLLLKNFKVLGSLSSSQCSQSLSVSQVHVDVHSHYNSVANETFCLEIMDSLRRCLSQQADVRLMLYEGFYDVLRRNSQLANSVMQTLLSQLKQFYEPKPDLLPPLKLEACILTQGDKISLQEPLDYLLCCIQHCLAWYKNTVIPLQQGEEEEEEEEAFYEDLDDILESITNRMIKSELEDFELDKSADFSQSTSIGIKNNICAFLVMGVCEVLIEYNFSISSFSKNRFEDILSLFMCYKKLSDILNEKAGKAKTKMANKTSDSLLSMKFVSSLLTALFRDSIQSHQESLSVLRSSNEFMRYAVNVALQKVQQLKETGHVSGPDGQNPEKIFQNLCDITRVLLWRYTSIPTSVEESGKKEKGKSISLLCLEGLQKIFSAVQQFYQPKIQQFLRALDVTDKEGEEREDADVSVTQRTAFQIRQFQRSLLNLLSSQEEDFNSKEALLLVTVLTSLSKLLEPSSPQFVQMLSWTSKICKENSREDALFCKSLMNLLFSLHVSYKSPVILLRDLSQDIHGHLGDIDQDVEVEKTNHFAIVNLRTAAPTVCLLVLSQAEKVLEEVDWLITKLKGQVSQETLSEEASSQATLPNQPVEKAIIMQLGTLLTFFHELVQTALPSGSCVDTLLKDLCKMYTTLTALVRYYLQVCQSSGGIPKNMEKLVKLSGSHLTPLCYSFISYVQNKSKSLNYTGEKKEKPAAVATAMARVLRETKPIPNLIFAIEQYEKFLIHLSKKSKVNLMQHMKLSTSRDFKIKGNILDMVLREDGEDENEEGTASEHGGQNKEPAKKKRKK
翻译修饰 - Q9NVI1 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K4 | Ubiquitination | Uniprot | |
| K12 | Ubiquitination | Uniprot | |
| K16 | Acetylation | Uniprot | |
| K16 | Ubiquitination | Uniprot | |
| K39 | Ubiquitination | Uniprot | |
| K41 | Ubiquitination | Uniprot | |
| K52 | Ubiquitination | Uniprot | |
| T62 | Phosphorylation | Uniprot | |
| K125 | Ubiquitination | Uniprot | |
| K141 | Ubiquitination | Uniprot | |
| K148 | Ubiquitination | Uniprot | |
| S152 | Phosphorylation | Uniprot | |
| K157 | Ubiquitination | Uniprot | |
| K158 | Ubiquitination | Uniprot | |
| Y215 | Phosphorylation | Uniprot | |
| S222 | Phosphorylation | Uniprot | |
| K331 | Ubiquitination | Uniprot | |
| K336 | Ubiquitination | Uniprot | |
| K339 | Ubiquitination | Uniprot | |
| K348 | Ubiquitination | Uniprot | |
| K402 | Ubiquitination | Uniprot | |
| T406 | Phosphorylation | Uniprot | |
| S407 | Phosphorylation | Uniprot | |
| S409 | Phosphorylation | Uniprot | |
| S411 | Phosphorylation | Uniprot | |
| K420 | Ubiquitination | Uniprot | |
| K431 | Ubiquitination | Uniprot | |
| K501 | Ubiquitination | Uniprot | |
| K523 | Ubiquitination | Uniprot | |
| S556 | Phosphorylation | Q13315 (ATM) | Uniprot |
| S559 | Phosphorylation | Q13315 (ATM) | Uniprot |
| S565 | Phosphorylation | Uniprot | |
| Y606 | Phosphorylation | Uniprot | |
| Y610 | Phosphorylation | Uniprot | |
| S617 | Phosphorylation | Uniprot | |
| K638 | Ubiquitination | Uniprot | |
| K646 | Acetylation | Uniprot | |
| T653 | Phosphorylation | Uniprot | |
| K715 | Ubiquitination | Uniprot | |
| K725 | Ubiquitination | Uniprot | |
| S726 | Phosphorylation | Uniprot | |
| S730 | Phosphorylation | Q13315 (ATM) | Uniprot |
| K780 | Ubiquitination | Uniprot | |
| S782 | Phosphorylation | Uniprot | |
| K788 | Ubiquitination | Uniprot | |
| K799 | Ubiquitination | Uniprot | |
| T800 | Phosphorylation | Uniprot | |
| K849 | Ubiquitination | Uniprot | |
| K854 | Ubiquitination | Uniprot | |
| K869 | Ubiquitination | Uniprot | |
| K897 | Ubiquitination | Uniprot | |
| K902 | Ubiquitination | Uniprot | |
| S903 | Phosphorylation | Uniprot | |
| K926 | Ubiquitination | Uniprot | |
| K939 | Ubiquitination | Uniprot | |
| T952 | Phosphorylation | Uniprot | |
| S972 | Phosphorylation | Q13315 (ATM) | Uniprot |
| S1000 | Phosphorylation | Uniprot | |
| K1015 | Ubiquitination | Uniprot | |
| S1027 | Phosphorylation | Uniprot | |
| K1040 | Ubiquitination | Uniprot | |
| K1068 | Ubiquitination | Uniprot | |
| K1105 | Ubiquitination | Uniprot | |
| K1107 | Ubiquitination | Uniprot | |
| S1120 | Phosphorylation | Uniprot | |
| S1121 | Phosphorylation | Q13315 (ATM) | Uniprot |
| K1196 | Ubiquitination | Uniprot | |
| K1199 | Ubiquitination | Uniprot | |
| K1221 | Ubiquitination | Uniprot | |
| K1229 | Ubiquitination | Uniprot | |
| K1232 | Ubiquitination | Uniprot | |
| T1238 | Phosphorylation | Uniprot | |
| K1248 | Ubiquitination | Uniprot | |
| K1272 | Ubiquitination | Uniprot | |
| K1280 | Ubiquitination | Uniprot | |
| K1290 | Ubiquitination | Uniprot | |
| S1312 | Phosphorylation | Uniprot |
研究背景
Plays an essential role in the repair of DNA double-strand breaks by homologous recombination and in the repair of interstrand DNA cross-links (ICLs) by promoting FANCD2 monoubiquitination by FANCL and participating in recruitment to DNA repair sites. Required for maintenance of chromosomal stability. Specifically binds branched DNA: binds both single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA). Participates in S phase and G2 phase checkpoint activation upon DNA damage.
Monoubiquitinated by FANCL on Lys-523 during S phase and upon genotoxic stress. Deubiquitinated by USP1 as cells enter G2/M, or once DNA repair is completed. Monoubiquitination requires the FANCA-FANCB-FANCC-FANCE-FANCF-FANCG-FANCM complex. Ubiquitination is required for binding to chromatin, DNA repair, and normal cell cycle progression. Monoubiquitination is stimulated by DNA-binding.
Phosphorylated in response to DNA damage by ATM and/or ATR.
Nucleus.
Note: Observed in spots localized in pairs on the sister chromatids of mitotic chromosome arms and not centromeres, one on each chromatids. These foci coincide with common fragile sites. They are frequently interlinked through BLM-associated ultra-fine DNA bridges.
Interacts with FANCD2; the interaction is direct. Interacts with FANCL. Interacts with MTMR15/FAN1. Interacts with POLN.
The C-terminal 30 residues are probably required for function in DNA repair.
研究领域
· Genetic Information Processing > Replication and repair > Fanconi anemia pathway.
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