产品: Nanog 抗体
货号: AF6535
描述: Rabbit polyclonal antibody to Nanog
应用: ELISA(peptide)
反应: Human, Mouse, Rat
分子量: 35kD(Calculated).
蛋白号: Q9H9S0
RRID: AB_2847259

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 100ul RMB¥ 2300 现货
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产品描述

来源:
Rabbit
应用:
ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
克隆:
Polyclonal
特异性:
Nanog Antibody detects endogenous levels of total Nanog.
RRID:
AB_2847259
引用格式: Affinity Biosciences Cat# AF6535, RRID:AB_2847259.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Embryonic stem cell specific homeobox protein (Nanog); ENK; FLJ12581; hNanog; Homeobox protein NANOG; Homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; NANOG; Nanog homeobox; NANOG_HUMAN;

抗原和靶标

免疫原:

A synthesized peptide derived from human Nanog.

Uniprot:
基因/基因ID:
表达:
Q9H9S0 NANOG_HUMAN:

Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.

序列:
MSVDPACPQSLPCFEASDCKESSPMPVICGPEENYPSLQMSSAEMPHTETVSPLPSSMDLLIQDSPDSSTSPKGKQPTSAEKSVAKKEDKVPVKKQKTRTVFSSTQLCVLNDRFQRQKYLSLQQMQELSNILNLSYKQVKTWFQNQRMKSKRWQKNNWPKNSNGVTQKASAPTYPSLYSSYHQGCLVNPTGNLPMWSNQTWNNSTWSNQTQNIQSWSNHSWNTQTWCTQSWNNQAWNSPFYNCGEESLQSCMQFQPNSPASDLEAALEAAGEGLNVIQQTTRYFSTPQTMDLFLNYSMNMQPEDV

翻译修饰 - Q9H9S0 作为底物

Site PTM Type Enzyme
S2 Phosphorylation
Y35 Phosphorylation Q05397 (PTK2)
T50 Phosphorylation
S52 Phosphorylation P28482 (MAPK1)
S56 Phosphorylation
S57 Phosphorylation
S65 Phosphorylation
T70 Phosphorylation
S71 Phosphorylation P28482 (MAPK1) , P06493 (CDK1)
T78 Phosphorylation Q02156 (PRKCE) , P28482 (MAPK1)
S79 Phosphorylation Q02156 (PRKCE)
S135 Phosphorylation Q02156 (PRKCE)
Y174 Phosphorylation Q05397 (PTK2)
T200 Phosphorylation Q02156 (PRKCE)
S258 Phosphorylation
T280 Phosphorylation Q02156 (PRKCE)
T286 Phosphorylation
T289 Phosphorylation

研究背景

功能:

Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Binds to the POU5F1/OCT4 promoter. Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.

亚基结构:

Interacts with SMAD1 and SALL4. Interacts with ZNF281/ZFP281 (By similarity). Interacts with PCGF1. Interacts with ESRRB; reciprocally modulates their transcriptional activities (By similarity).

蛋白家族:

Belongs to the Nanog homeobox family.

研究领域

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.   (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

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