| 产品: | RapGEF1 抗体 |
| 货号: | AF6597 |
| 描述: | Rabbit polyclonal antibody to RapGEF1 |
| 应用: | ELISA(peptide) |
| 反应: | Human |
| 分子量: | 121kD(Calculated). |
| 蛋白号: | Q13905 |
| RRID: | AB_2847321 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF6597, RRID:AB_2847321.
展开/折叠
C3G; C3G protein; CRK SH3 binding GNRP; CRK SH3-binding GNRP; DKFZp781P1719; GRF 2; GRF2; Guanine nucleotide releasing factor 2 (specific for crk proto oncogene); Guanine nucleotide releasing factor 2; Guanine nucleotide-releasing factor 2; OTTHUMP00000064558; Protein C3G; Rap guanine nucleotide exchange factor (GEF) 1; Rap guanine nucleotide exchange factor 1; RAPGEF 1; RAPGEF1; RPGF1_HUMAN;
抗原和靶标
A synthesized peptide derived from human RapGEF1.
Ubiquitously expressed in adult and fetus. Expression is high in adult skeletal muscle and placenta and in fetal brain and heart. Low levels of expression in adult and fetal liver.
- Q13905 RPGF1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDTDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKEATDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFLGPPFQLPLGGHPQPDGPLAPGQQTDTPPALPEKKRRSAASQTADGSGCRVSYERHPSQYDNISGEDLQSTAPIPSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLEPPAGKDGHPRDPSAVSGVPGKDSRDGSERAPKSPDALESAQSEEEVDELSLIDHNEIMSRLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKLLRCATSSQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDMRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT
翻译修饰 - Q13905 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S12 | Phosphorylation | Uniprot | |
| S23 | Phosphorylation | Uniprot | |
| Y61 | Phosphorylation | Uniprot | |
| S76 | Phosphorylation | Uniprot | |
| T109 | Phosphorylation | Uniprot | |
| K200 | Ubiquitination | Uniprot | |
| S214 | Phosphorylation | Uniprot | |
| S217 | Phosphorylation | Uniprot | |
| S222 | Phosphorylation | Uniprot | |
| S223 | Phosphorylation | Uniprot | |
| S253 | Phosphorylation | Uniprot | |
| T261 | Phosphorylation | Uniprot | |
| S281 | Phosphorylation | Uniprot | |
| S293 | Phosphorylation | Uniprot | |
| S296 | Phosphorylation | Uniprot | |
| S307 | Phosphorylation | Uniprot | |
| T310 | Phosphorylation | Uniprot | |
| S311 | Phosphorylation | Uniprot | |
| S313 | Phosphorylation | Uniprot | |
| S314 | Phosphorylation | Uniprot | |
| Y329 | Phosphorylation | Uniprot | |
| S335 | Phosphorylation | Uniprot | |
| S338 | Phosphorylation | Uniprot | |
| Y341 | Phosphorylation | Uniprot | |
| S345 | Phosphorylation | Uniprot | |
| S349 | Phosphorylation | Uniprot | |
| S352 | Phosphorylation | Uniprot | |
| S353 | Phosphorylation | Uniprot | |
| S358 | Phosphorylation | Uniprot | |
| S360 | Phosphorylation | Uniprot | |
| S366 | Phosphorylation | Uniprot | |
| S371 | Phosphorylation | Uniprot | |
| Y390 | Phosphorylation | Uniprot | |
| S423 | Phosphorylation | Uniprot | |
| S463 | Phosphorylation | Uniprot | |
| S466 | Phosphorylation | Uniprot | |
| Y504 | Phosphorylation | P00519 (ABL1) , P12931 (SRC) , P08631 (HCK) | Uniprot |
| Y561 | Phosphorylation | Uniprot | |
| Y570 | Phosphorylation | Uniprot | |
| T572 | Phosphorylation | Uniprot | |
| Y579 | Phosphorylation | Uniprot | |
| S652 | Phosphorylation | Uniprot | |
| K683 | Ubiquitination | Uniprot | |
| Y715 | Phosphorylation | Uniprot | |
| Y723 | Phosphorylation | Uniprot | |
| K808 | Ubiquitination | Uniprot | |
| T819 | Phosphorylation | Uniprot | |
| S820 | Phosphorylation | Uniprot | |
| K869 | Ubiquitination | Uniprot | |
| T950 | Phosphorylation | Uniprot | |
| S951 | Phosphorylation | Uniprot | |
| S965 | Phosphorylation | Uniprot | |
| S966 | Phosphorylation | Uniprot | |
| T1067 | Phosphorylation | Uniprot | |
| T1071 | Phosphorylation | Uniprot |
研究背景
Guanine nucleotide-releasing protein that binds to SH3 domain of CRK and GRB2/ASH. Transduces signals from CRK to activate RAS. Plays a role in the establishment of basal endothelial barrier function. Plays a role in nerve growth factor (NGF)-induced sustained activation of Rap1 and neurite outgrowth.
Phosphorylation at Tyr-504 enhances activity as Rap guanine nucleotide exchange factor.
Early endosome.
Ubiquitously expressed in adult and fetus. Expression is high in adult skeletal muscle and placenta and in fetal brain and heart. Low levels of expression in adult and fetal liver.
Interacts with CRK via its SH3-binding sites.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)
· Environmental Information Processing > Signal transduction > Rap1 signaling pathway. (View pathway)
· Human Diseases > Cancers: Specific types > Renal cell carcinoma. (View pathway)
· Organismal Systems > Nervous system > Neurotrophin signaling pathway. (View pathway)
· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)
限制条款
产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。
产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。
Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。产品仅供科学研究使用。不用于诊断和治疗。
产品未经授权不得转售。
Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.