产品: MCL1 抗体
货号: AF6613
描述: Rabbit polyclonal antibody to MCL1
应用: WB ELISA(peptide)
反应: Human, Mouse, Rat
分子量: 37kD(Calculated).
蛋白号: Q07820
RRID: AB_2847336

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
克隆:
Polyclonal
特异性:
MCL1 Antibody detects endogenous levels of total MCL1.
RRID:
AB_2847336
引用格式: Affinity Biosciences Cat# AF6613, RRID:AB_2847336.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Bcl 2 related protein EAT/mcl1; Bcl-2-like protein 3; Bcl-2-related protein EAT/mcl1; BCL2 related; Bcl2-L-3; BCL2L3; EAT; Induced myeloid leukemia cell differentiation protein Mcl 1; Induced myeloid leukemia cell differentiation protein Mcl-1; MCL 1; MCL1; MCL1-ES; mcl1/EAT; MCL1_HUMAN; MCL1L; MCL1S; MGC104264; MGC1839; Myeloid Cell Leukemia 1; Myeloid cell leukemia ES; Myeloid cell leukemia sequence 1; Myeloid cell leukemia sequence 1 BCL2 related; Myeloid cell leukemia sequence 1 isoform 1; OTTHUMP00000032794; OTTHUMP00000032795; TM;

抗原和靶标

免疫原:

A synthesized peptide derived from human MCL1.

Uniprot:
基因/基因ID:
序列:
MFGLKRNAVIGLNLYCGGAGLGAGSGGATRPGGRLLATEKEASARREIGGGEAGAVIGGSAGASPPSTLTPDSRRVARPPPIGAEVPDVTATPARLLFFAPTRRAAPLEEMEAPAADAIMSPEEELDGYEPEPLGKRPAVLPLLELVGESGNNTSTDGSLPSTPPPAEEEEDELYRQSLEIISRYLREQATGAKDTKPMGRSGATSRKALETLRRVGDGVQRNHETAFQGMLRKLDIKNEDDVKSLSRVMIHVFSDGVTNWGRIVTLISFGAFVAKHLKTINQESCIEPLAESITDVLVRTKRDWLVKQRGWDGFVEFFHVEDLEGGIRNVLLAFAGVAGVGAGLAYLIR

翻译修饰 - Q07820 作为底物

Site PTM Type Enzyme
K5 Methylation
K5 Ubiquitination
K40 Ubiquitination
S64 Phosphorylation P45983 (MAPK8) , P24941 (CDK2) , P06493 (CDK1)
T68 Phosphorylation
T70 Phosphorylation
T92 Phosphorylation P06493 (CDK1) , P27361 (MAPK3) , P24941 (CDK2)
S121 Phosphorylation P45983 (MAPK8) , P24941 (CDK2) , P53779 (MAPK10)
Y129 Phosphorylation
K136 Ubiquitination
S150 Phosphorylation
T154 Phosphorylation
T156 Phosphorylation
S159 Phosphorylation P49840 (GSK3A) , P49841 (GSK3B)
S162 Phosphorylation
T163 Phosphorylation P24941 (CDK2) , P53779 (MAPK10) , P27361 (MAPK3) , P45983 (MAPK8) , P28482 (MAPK1)
K194 Ubiquitination
K197 Ubiquitination
K234 Ubiquitination
K238 Ubiquitination
K244 Ubiquitination
S245 Phosphorylation
K276 Ubiquitination
K279 Ubiquitination
K302 Ubiquitination
K308 Ubiquitination
Y347 Phosphorylation

研究背景

功能:

Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis.

翻译修饰:

Cleaved by CASP3 during apoptosis. In intact cells cleavage occurs preferentially after Asp-127, yielding a pro-apoptotic 28 kDa C-terminal fragment.

Rapidly degraded in the absence of phosphorylation on Thr-163 in the PEST region.

Phosphorylated on Ser-159, by GSK3, in response to IL3/interleukin-3 withdrawal. Phosphorylation at Ser-159 induces ubiquitination and proteasomal degradation, abrogating the anti-apoptotic activity. Treatment with taxol or okadaic acid induces phosphorylation on additional sites.

Ubiquitinated. Ubiquitination is induced by phosphorylation at Ser-159.

细胞定位:

Membrane>Single-pass membrane protein. Cytoplasm. Mitochondrion. Nucleus>Nucleoplasm.
Note: Cytoplasmic, associated with mitochondria.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Interacts with HIF3A (via C-terminus domain) (By similarity). Interacts with BAD, BOK, BIK and BMF (By similarity). Interacts with PMAIP1. Interacts with BBC3 (By similarity). Isoform 1 interacts with BAX, BAK1 and TPT1. Heterodimer of isoform 1 and isoform 2. Homodimers of isoform 1 or isoform 2 are not detected. Isoform 2 does not interact with pro-apoptotic BCL2-related proteins. Interacts with RTL10/BOP. Interacts with BCL2L11; may sequester BCL2L11 to prevent its pro-apoptotic activity. Interacts with GIMAP5 and HSPA8/HSC70; the interaction between HSPA8 and MCL1 is impaired in the absence of GIMAP5 (By similarity).

蛋白家族:

Belongs to the Bcl-2 family.

研究领域

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

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