MLK1/2 Antibody - #AF6630

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产品: MLK1/2 抗体
货号: AF6630
描述: Rabbit polyclonal antibody to MLK1/2
应用: WB ELISA(peptide)
反应: Human, Mouse, Rat
分子量: 122kD,104kD(Calculated).
蛋白号: P80192 | Q02779
RRID: AB_2847353

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   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
克隆:
Polyclonal
特异性:
MLK1/2 Antibody detects endogenous levels of total MLK1/2.
RRID:
AB_2847353
引用格式: Affinity Biosciences Cat# AF6630, RRID:AB_2847353.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

M3K9_HUMAN; Map3k9; MEKK9; Mitogen activated protein kinase kinase kinase 9; Mitogen-activated protein kinase kinase kinase 9; Mixed lineage kinase 1 (tyr and ser/thr specificity); Mixed lineage kinase 1; MLK1; PRKE1; M3K10_HUMAN; MAP3K 10; Map3k10; MEKK10; Mitogen activated protein kinase kinase kinase 10; Mitogen-activated protein kinase kinase kinase 10; Mixed lineage kinase 2; MKN28 derived nonreceptor type serine/threonine kinase; MKN28 kinase; MLK 2; MST; Protein kinase MST;

抗原和靶标

免疫原:

A synthesized peptide derived from human MLK1/2.

Uniprot:

P80192(M3K9_HUMAN) >>Visit HPA database.

Q02779(M3K10_HUMAN) >>Visit HPA database.

基因/基因ID:
MAP3K9(4293) | MAP3K10(4294)
表达:
P80192 M3K9_HUMAN:

Expressed in epithelial tumor cell lines of colonic, breast and esophageal origin.

Q02779 M3K10_HUMAN:

Expressed in brain and skeletal muscle.

序列:
MEPSRALLGCLASAAAAAPPGEDGAGAGAEEEEEEEEEAAAAVGPGELGCDAPLPYWTAVFEYEAAGEDELTLRLGDVVEVLSKDSQVSGDEGWWTGQLNQRVGIFPSNYVTPRSAFSSRCQPGGEDPSCYPPIQLLEIDFAELTLEEIIGIGGFGKVYRAFWIGDEVAVKAARHDPDEDISQTIENVRQEAKLFAMLKHPNIIALRGVCLKEPNLCLVMEFARGGPLNRVLSGKRIPPDILVNWAVQIARGMNYLHDEAIVPIIHRDLKSSNILILQKVENGDLSNKILKITDFGLAREWHRTTKMSAAGTYAWMAPEVIRASMFSKGSDVWSYGVLLWELLTGEVPFRGIDGLAVAYGVAMNKLALPIPSTCPEPFAKLMEDCWNPDPHSRPSFTNILDQLTTIEESGFFEMPKDSFHCLQDNWKHEIQEMFDQLRAKEKELRTWEEELTRAALQQKNQEELLRRREQELAEREIDILERELNIIIHQLCQEKPRVKKRKGKFRKSRLKLKDGNRISLPSDFQHKFTVQASPTMDKRKSLINSRSSPPASPTIIPRLRAIQLTPGESSKTWGRSSVVPKEEGEEEEKRAPKKKGRTWGPGTLGQKELASGDEGSPQRREKANGLSTPSESPHFHLGLKSLVDGYKQWSSSAPNLVKGPRSSPALPGFTSLMEMEDEDSEGPGSGESRLQHSPSQSYLCIPFPRGEDGDGPSSDGIHEEPTPVNSATSTPQLTPTNSLKRGGAHHRRCEVALLGCGAVLAATGLGFDLLEAGKCQLLPLEEPEPPAREEKKRREGLFQRSSRPRRSTSPPSRKLFKKEEPMLLLGDPSASLTLLSLSSISECNSTRSLLRSDSDEIVVYEMPVSPVEAPPLSPCTHNPLVNVRVERFKRDPNQSLTPTHVTLTTPSQPSSHRRTPSDGALKPETLLASRSPSSNGLSPSPGAGMLKTPSPSRDPGEFPRLPDPNVVFPPTPRRWNTQQDSTLERPKTLEFLPRPRPSANRQRLDPWWFVSPSHARSTSPANSSSTETPSNLDSCFASSSSTVEERPGLPALLPFQAGPLPPTERTLLDLDAEGQSQDSTVPLCRAELNTHRPAPYEIQQEFWS

MEEEEGAVAKEWGTTPAGPVWTAVFDYEAAGDEELTLRRGDRVQVLSQDCAVSGDEGWWTGQLPSGRVGVFPSNYVAPGAPAAPAGLQLPQEIPFHELQLEEIIGVGGFGKVYRALWRGEEVAVKAARLDPEKDPAVTAEQVCQEARLFGALQHPNIIALRGACLNPPHLCLVMEYARGGALSRVLAGRRVPPHVLVNWAVQVARGMNYLHNDAPVPIIHRDLKSINILILEAIENHNLADTVLKITDFGLAREWHKTTKMSAAGTYAWMAPEVIRLSLFSKSSDVWSFGVLLWELLTGEVPYREIDALAVAYGVAMNKLTLPIPSTCPEPFARLLEECWDPDPHGRPDFGSILKRLEVIEQSALFQMPLESFHSLQEDWKLEIQHMFDDLRTKEKELRSREEELLRAAQEQRFQEEQLRRREQELAEREMDIVERELHLLMCQLSQEKPRVRKRKGNFKRSRLLKLREGGSHISLPSGFEHKITVQASPTLDKRKGSDGASPPASPSIIPRLRAIRLTPVDCGGSSSGSSSGGSGTWSRGGPPKKEELVGGKKKGRTWGPSSTLQKERVGGEERLKGLGEGSKQWSSSAPNLGKSPKHTPIAPGFASLNEMEEFAEAEDGGSSVPPSPYSTPSYLSVPLPAEPSPGARAPWEPTPSAPPARWGHGARRRCDLALLGCATLLGAVGLGADVAEARAADGEEQRRWLDGLFFPRAGRFPRGLSPPARPHGRREDVGPGLGLAPSATLVSLSSVSDCNSTRSLLRSDSDEAAPAAPSPPPSPPAPTPTPSPSTNPLVDLELESFKKDPRQSLTPTHVTAACAVSRGHRRTPSDGALGQRGPPEPAGHGPGPRDLLDFPRLPDPQALFPARRRPPEFPGRPTTLTFAPRPRPAASRPRLDPWKLVSFGRTLTISPPSRPDTPESPGPPSVQPTLLDMDMEGQNQDSTVPLCGAHGSH

翻译修饰 - P80192/Q02779 作为底物

Site PTM Type Enzyme
Y110 Phosphorylation
K193 Ubiquitination
T304 Phosphorylation P80192 (MAP3K9)
T305 Phosphorylation P80192 (MAP3K9)
S308 Phosphorylation P80192 (MAP3K9)
T312 Phosphorylation P80192 (MAP3K9)
T452 Phosphorylation
S519 Phosphorylation
S533 Phosphorylation
T535 Phosphorylation
S547 Phosphorylation
S548 Phosphorylation
S552 Phosphorylation
T554 Phosphorylation
T565 Phosphorylation
K594 Acetylation
K595 Acetylation
K607 Acetylation
S616 Phosphorylation
S652 Phosphorylation
K658 Ubiquitination
S693 Phosphorylation
S695 Phosphorylation
S807 Phosphorylation
T808 Phosphorylation
S809 Phosphorylation
S812 Phosphorylation
T915 Phosphorylation
S917 Phosphorylation
S938 Phosphorylation
T948 Phosphorylation
Site PTM Type Enzyme
S225 Phosphorylation
S446 Phosphorylation
S489 Phosphorylation
S498 Phosphorylation
S502 Phosphorylation
S506 Phosphorylation
S508 Phosphorylation
T558 Phosphorylation
S589 Phosphorylation
S722 Phosphorylation
S809 Phosphorylation
S830 Phosphorylation

翻译修饰 - P80192/Q02779 作为激酶

Substrate Site Source
P80192 (MAP3K9) T304 Uniprot
P80192 (MAP3K9) T305 Uniprot
P80192 (MAP3K9) S308 Uniprot
P80192 (MAP3K9) T312 Uniprot
Substrate Site Source
P13688-1 (CEACAM1) S508 Uniprot
P15923 (TCF3) S341 Uniprot
P15923 (TCF3) S352 Uniprot
P15923 (TCF3) T355 Uniprot
P15923 (TCF3) S359 Uniprot
P15923 (TCF3) S379 Uniprot
P45985 (MAP2K4) T261 Uniprot
Q92630 (DYRK2) T381 Uniprot
Q92630 (DYRK2) S449 Uniprot

研究背景

功能:

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade through the phosphorylation of MAP2K4/MKK4 and MAP2K7/MKK7 which in turn activate the JNKs. The MKK/JNK signaling pathway regulates stress response via activator protein-1 (JUN) and GATA4 transcription factors. Plays also a role in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis.

翻译修饰:

Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. Thr-312 is likely to be the main autophosphorylation site. Autophosphorylation also occurs on Thr-304 and Ser-308.

组织特异性:

Expressed in epithelial tumor cell lines of colonic, breast and esophageal origin.

亚基结构:

Homodimer.

蛋白家族:

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.

功能:

Activates the JUN N-terminal pathway.

翻译修饰:

Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation.

组织特异性:

Expressed in brain and skeletal muscle.

亚基结构:

Homodimer. Interacts with SH3RF2.

蛋白家族:

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.

限制条款

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