| 产品: | JAK1 抗体 |
| 货号: | AF6701 |
| 描述: | Rabbit polyclonal antibody to JAK1 |
| 应用: | IF/ICC |
| 反应: | Human, Mouse, Rat |
| 分子量: | 133kD(Calculated). |
| 蛋白号: | P23458 |
| RRID: | AB_2847424 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF6701, RRID:AB_2847424.
展开/折叠
JAK 1; JAK 1A; JAK 1B; JAK-1; JAK1; JAK1_HUMAN; JAK1A; JAK1B; Janus kinase 1 (a protein tyrosine kinase); Janus kinase 1; JTK3; Tyrosine protein kinase JAK 1; Tyrosine protein kinase JAK1; Tyrosine-protein kinase JAK1;
抗原和靶标
A synthesized peptide derived from human JAK1.
Expressed at higher levels in primary colon tumors than in normal colon tissue. The expression level in metastatic colon tumors is comparable to the expression level in normal colon tissue.
- P23458 JAK1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MQYLNIKEDCNAMAFCAKMRSSKKTEVNLEAPEPGVEVIFYLSDREPLRLGSGEYTAEELCIRAAQACRISPLCHNLFALYDENTKLWYAPNRTITVDDKMSLRLHYRMRFYFTNWHGTNDNEQSVWRHSPKKQKNGYEKKKIPDATPLLDASSLEYLFAQGQYDLVKCLAPIRDPKTEQDGHDIENECLGMAVLAISHYAMMKKMQLPELPKDISYKRYIPETLNKSIRQRNLLTRMRINNVFKDFLKEFNNKTICDSSVSTHDLKVKYLATLETLTKHYGAEIFETSMLLISSENEMNWFHSNDGGNVLYYEVMVTGNLGIQWRHKPNVVSVEKEKNKLKRKKLENKHKKDEEKNKIREEWNNFSYFPEITHIVIKESVVSINKQDNKKMELKLSSHEEALSFVSLVDGYFRLTADAHHYLCTDVAPPLIVHNIQNGCHGPICTEYAINKLRQEGSEEGMYVLRWSCTDFDNILMTVTCFEKSEQVQGAQKQFKNFQIEVQKGRYSLHGSDRSFPSLGDLMSHLKKQILRTDNISFMLKRCCQPKPREISNLLVATKKAQEWQPVYPMSQLSFDRILKKDLVQGEHLGRGTRTHIYSGTLMDYKDDEGTSEEKKIKVILKVLDPSHRDISLAFFEAASMMRQVSHKHIVYLYGVCVRDVENIMVEEFVEGGPLDLFMHRKSDVLTTPWKFKVAKQLASALSYLEDKDLVHGNVCTKNLLLAREGIDSECGPFIKLSDPGIPITVLSRQECIERIPWIAPECVEDSKNLSVAADKWSFGTTLWEICYNGEIPLKDKTLIEKERFYESRCRPVTPSCKELADLMTRCMNYDPNQRPFFRAIMRDINKLEEQNPDIVSEKKPATEVDPTHFEKRFLKRIRDLGEGHFGKVELCRYDPEGDNTGEQVAVKSLKPESGGNHIADLKKEIEILRNLYHENIVKYKGICTEDGGNGIKLIMEFLPSGSLKEYLPKNKNKINLKQQLKYAVQICKGMDYLGSRQYVHRDLAARNVLVESEHQVKIGDFGLTKAIETDKEYYTVKDDRDSPVFWYAPECLMQSKFYIASDVWSFGVTLHELLTYCDSDSSPMALFLKMIGPTHGQMTVTRLVNTLKEGKRLPCPPNCPDEVYQLMRKCWEFQPSNRTSFQNLIEGFEALLK
翻译修饰 - P23458 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Phosphorylation | Uniprot | ||
| M1 | Acetylation | Uniprot | |
| Y3 | Phosphorylation | Uniprot | |
| K100 | Ubiquitination | Uniprot | |
| T119 | Phosphorylation | Uniprot | |
| S125 | Phosphorylation | Uniprot | |
| K142 | Ubiquitination | Uniprot | |
| K213 | Ubiquitination | Uniprot | |
| S216 | Phosphorylation | Uniprot | |
| Y217 | Phosphorylation | Uniprot | |
| Y220 | Phosphorylation | Uniprot | |
| K227 | Ubiquitination | Uniprot | |
| S228 | Phosphorylation | Uniprot | |
| K245 | Ubiquitination | Uniprot | |
| K249 | Ubiquitination | Uniprot | |
| K254 | Ubiquitination | Uniprot | |
| K267 | Ubiquitination | Uniprot | |
| K269 | Acetylation | Uniprot | |
| K269 | Ubiquitination | Uniprot | |
| S333 | Phosphorylation | Uniprot | |
| K386 | Ubiquitination | Uniprot | |
| Y412 | Phosphorylation | Uniprot | |
| K493 | Ubiquitination | Uniprot | |
| K496 | Ubiquitination | Uniprot | |
| K504 | Ubiquitination | Uniprot | |
| Y507 | Phosphorylation | Uniprot | |
| S515 | Phosphorylation | Uniprot | |
| S518 | Phosphorylation | Uniprot | |
| S524 | Phosphorylation | Uniprot | |
| K541 | Ubiquitination | Uniprot | |
| K559 | Ubiquitination | Uniprot | |
| Y568 | Phosphorylation | Uniprot | |
| S574 | Phosphorylation | Uniprot | |
| K581 | Ubiquitination | Uniprot | |
| Y605 | Phosphorylation | Uniprot | |
| K606 | Ubiquitination | Uniprot | |
| T687 | Phosphorylation | Uniprot | |
| T688 | Phosphorylation | Uniprot | |
| K691 | Ubiquitination | Uniprot | |
| K696 | Ubiquitination | Uniprot | |
| K708 | Ubiquitination | Uniprot | |
| K718 | Ubiquitination | Uniprot | |
| K736 | Ubiquitination | Uniprot | |
| S738 | Phosphorylation | Uniprot | |
| K768 | Ubiquitination | Uniprot | |
| K802 | Ubiquitination | Uniprot | |
| K847 | Ubiquitination | Uniprot | |
| S857 | Phosphorylation | Uniprot | |
| K860 | Ubiquitination | Uniprot | |
| K872 | Ubiquitination | Uniprot | |
| K888 | Ubiquitination | Uniprot | |
| K908 | Ubiquitination | Uniprot | |
| S909 | Phosphorylation | Uniprot | |
| K911 | Ubiquitination | Uniprot | |
| K923 | Ubiquitination | Uniprot | |
| K924 | Ubiquitination | Uniprot | |
| K939 | Ubiquitination | Uniprot | |
| K941 | Ubiquitination | Uniprot | |
| Y967 | Phosphorylation | Uniprot | |
| K978 | Ubiquitination | Uniprot | |
| Y993 | Phosphorylation | Uniprot | |
| K1018 | Ubiquitination | Uniprot | |
| K1026 | Ubiquitination | Uniprot | |
| T1030 | Phosphorylation | Uniprot | |
| K1032 | Ubiquitination | Uniprot | |
| Y1034 | Phosphorylation | P52333 (JAK3) , P23458 (JAK1) | Uniprot |
| Y1035 | Phosphorylation | P23458 (JAK1) , P52333 (JAK3) | Uniprot |
| T1036 | Phosphorylation | Uniprot | |
| K1038 | Ubiquitination | Uniprot | |
| S1043 | Phosphorylation | Uniprot | |
| T1107 | Phosphorylation | Uniprot | |
| K1109 | Acetylation | Uniprot | |
| K1109 | Ubiquitination | Uniprot | |
| K1112 | Acetylation | Uniprot | |
| Y1125 | Phosphorylation | Uniprot | |
| K1130 | Ubiquitination | Uniprot |
翻译修饰 - P23458 作为激酶
| Substrate | Site | Source |
|---|---|---|
| P14784 (IL2RB) | Y364 | Uniprot |
| P15260 (IFNGR1) | Y457 | Uniprot |
| P19525-1 (EIF2AK2) | Y101 | Uniprot |
| P19525-1 (EIF2AK2) | Y293 | Uniprot |
| P23458 (JAK1) | Y1034 | Uniprot |
| P23458 (JAK1) | Y1035 | Uniprot |
| P29597 (TYK2) | Y1054 | Uniprot |
| P29597 (TYK2) | Y1055 | Uniprot |
| P35568 (IRS1) | Y612 | Uniprot |
| P35568 (IRS1) | Y632 | Uniprot |
| P35568 (IRS1) | Y662 | Uniprot |
| P35568 (IRS1) | Y732 | Uniprot |
| P40763-2 (STAT3) | Y704 | Uniprot |
| P40763 (STAT3) | Y705 | Uniprot |
| P42224-2 (STAT1) | Y701 | Uniprot |
| P52630-3 (STAT2) | Y690 | Uniprot |
| Q13651 (IL10RA) | Y446 | Uniprot |
| Q13651 (IL10RA) | Y496 | Uniprot |
| Q99683 (MAP3K5) | Y718 | Uniprot |
研究背景
Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway. Kinase partner for the interleukin (IL)-2 receptor as well as interleukin (IL)-10 receptor.
Autophosphorylated. Phosphorylated on tyrosine residues in response to interferon gamma signaling. Dephosphorylation of Tyr-1034 and Tyr-1035 by PTPN2 negatively regulates cytokine-mediated signaling.
Ubiquitinated by RNF125; leading to its degradation by the proteasome.
Endomembrane system>Peripheral membrane protein.
Note: Wholly intracellular, possibly membrane associated.
Expressed at higher levels in primary colon tumors than in normal colon tissue. The expression level in metastatic colon tumors is comparable to the expression level in normal colon tissue.
Interacts with IL31RA. Interacts with IFNAR2. Interacts with IFNGR1. Interacts with JAKMIP1. Interacts with SHB. Interacts (via N-terminus) with IL2RB and IL10RA (via its cytoplasmic domain). Interacts with FER (By similarity).
Possesses two phosphotransferase domains. The second one probably contains the catalytic domain, while the presence of slight differences suggest a different role for domain 1.
The FERM domain mediates interaction with JAKMIP1.
Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.
研究领域
· Cellular Processes > Cell growth and death > Necroptosis. (View pathway)
· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells. (View pathway)
· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway. (View pathway)
· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.
· Human Diseases > Infectious diseases: Parasitic > Leishmaniasis.
· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.
· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.
· Human Diseases > Infectious diseases: Viral > Hepatitis C.
· Human Diseases > Infectious diseases: Viral > Hepatitis B.
· Human Diseases > Infectious diseases: Viral > Measles.
· Human Diseases > Infectious diseases: Viral > Influenza A.
· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.
· Human Diseases > Infectious diseases: Viral > HTLV-I infection.
· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.
· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Human Diseases > Cancers: Overview > Viral carcinogenesis.
· Human Diseases > Cancers: Specific types > Pancreatic cancer. (View pathway)
· Organismal Systems > Development > Osteoclast differentiation. (View pathway)
· Organismal Systems > Immune system > NOD-like receptor signaling pathway. (View pathway)
· Organismal Systems > Immune system > Th1 and Th2 cell differentiation. (View pathway)
· Organismal Systems > Immune system > Th17 cell differentiation. (View pathway)
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