| 产品: | NFAT5 抗体 |
| 货号: | AF6798 |
| 描述: | Rabbit polyclonal antibody to NFAT5 |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse, Rat, Monkey |
| 分子量: | 160kD; 166kD(Calculated). |
| 蛋白号: | O94916 |
| RRID: | AB_2847521 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF6798, RRID:AB_2847521.
展开/折叠
Glutamine rich protein H65; KIAA0827; NF AT5; NF-AT5; NFAT 5; NFAT L1; NFAT like protein 1; NFAT5; NFAT5_HUMAN; NFATL 1; NFATL1; NFATZ; Nuclear factor of activated T cells 5; Nuclear factor of activated T cells 5 tonicity responsive; Nuclear factor of activated T cells; Nuclear factor of activated T-cells 5; OREBP; Osmotic response element binding protein; T cell transcription factor NFAT 5; T cell transcription factor NFAT5; T-cell transcription factor NFAT5; TonE binding protein; TonE-binding protein; TonEBP; Tonicity responsive enhancer binding protein; Tonicity-responsive enhancer-binding protein;
抗原和靶标
A synthesized peptide derived from human NFAT5.
Widely expressed, with highest levels in skeletal muscle, brain, heart and peripheral blood leukocytes.
- O94916 NFAT5_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPSDFISLLSADLDLESPKSLYSRESVYDLLPKELQLPPSRETSVASMSQTSGGEAGSPPPAVVAADASSAPSSSSMGGACSSFTTSSSPTIYSTSVTDSKAMQVESCSSAVGVSNRGVSEKQLTSNTVQQHPSTPKRHTVLYISPPPEDLLDNSRMSCQDEGCGLESEQSCSMWMEDSPSNFSNMSTSSYNDNTEVPRKSRKRNPKQRPGVKRRDCEESNMDIFDADSAKAPHYVLSQLTTDNKGNSKAGNGTLENQKGTGVKKSPMLCGQYPVKSEGKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNEPVVLQVFVGNDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVGLDPSNNMTLAVDCVGILKLRNADVEARIGIAGSKKKSTRARLVFRVNIMRKDGSTLTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLKGTKVIFQENVSDENSWKSEAEIDMELFHQNHLIVKVPPYHDQHITLPVSVGIYVVTNAGRSHDVQPFTYTPDPAAAGALNVNVKKEISSPARPCSFEEAMKAMKTTGCNLDKVNIIPNALMTPLIPSSMIKSEDVTPMEVTAEKRSSTIFKTTKSVGSTQQTLENISNIAGNGSFSSPSSSHLPSENEKQQQIQPKAYNPETLTTIQTQDISQPGTFPAVSASSQLPNSDALLQQATQFQTRETQSREILQSDGTVVNLSQLTEASQQQQQSPLQEQAQTLQQQISSNIFPSPNSVSQLQNTIQQLQAGSFTGSTASGSSGSVDLVQQVLEAQQQLSSVLFSAPDGNENVQEQLSADIFQQVSQIQSGVSPGMFSSTEPTVHTRPDNLLPGRAESVHPQSENTLSNQQQQQQQQQQVMESSAAMVMEMQQSICQAAAQIQSELFPSTASANGNLQQSPVYQQTSHMMSALSTNEDMQMQCELFSSPPAVSGNETSTTTTQQVATPGTTMFQTSSSGDGEETGTQAKQIQNSVFQTMVQMQHSGDNQPQVNLFSSTKSMMSVQNSGTQQQGNGLFQQGNEMMSLQSGNFLQQSSHSQAQLFHPQNPIADAQNLSQETQGSLFHSPNPIVHSQTSTTSSEQMQPPMFHSQSTIAVLQGSSVPQDQQSTNIFLSQSPMNNLQTNTVAQEAFFAAPNSISPLQSTSNSEQQAAFQQQAPISHIQTPMLSQEQAQPPQQGLFQPQVALGSLPPNPMPQSQQGTMFQSQHSIVAMQSNSPSQEQQQQQQQQQQQQQQQQQSILFSNQNTMATMASPKQPPPNMIFNPNQNPMANQEQQNQSIFHQQSNMAPMNQEQQPMQFQSQSTVSSLQNPGPTQSESSQTPLFHSSPQIQLVQGSPSSQEQQVTLFLSPASMSALQTSINQQDMQQSPLYSPQNNMPGIQGATSSPQPQATLFHNTAGGTMNQLQNSPGSSQQTSGMFLFGIQNNCSQLLTSGPATLPDQLMAISQPGQPQNEGQPPVTTLLSQQMPENSPLASSINTNQNIEKIDLLVSLQNQGNNLTGSF
翻译修饰 - O94916 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S17 | Phosphorylation | Uniprot | |
| Y28 | Phosphorylation | Uniprot | |
| S107 | Phosphorylation | Uniprot | |
| C108 | S-Nitrosylation | Uniprot | |
| S109 | Phosphorylation | Uniprot | |
| S110 | Phosphorylation | Uniprot | |
| S115 | Phosphorylation | Uniprot | |
| S120 | Phosphorylation | Uniprot | |
| K122 | Acetylation | Uniprot | |
| K122 | Ubiquitination | Uniprot | |
| T125 | Phosphorylation | Uniprot | |
| T128 | Phosphorylation | Uniprot | |
| S134 | Phosphorylation | Uniprot | |
| T135 | Phosphorylation | P06493 (CDK1) , Q00535 (CDK5) | Uniprot |
| K137 | Ubiquitination | Uniprot | |
| T140 | Phosphorylation | Uniprot | |
| Y143 | Phosphorylation | P00519 (ABL1) | Uniprot |
| S145 | Phosphorylation | Uniprot | |
| S155 | Phosphorylation | Uniprot | |
| S158 | Phosphorylation | Q8N752 (CSNK1A1L) , P48729 (CSNK1A1) | Uniprot |
| S220 | Phosphorylation | Uniprot | |
| S229 | Phosphorylation | Uniprot | |
| Y235 | Phosphorylation | Uniprot | |
| S238 | Phosphorylation | Uniprot | |
| T241 | Phosphorylation | Uniprot | |
| S248 | Phosphorylation | Uniprot | |
| S266 | Phosphorylation | Uniprot | |
| K276 | Sumoylation | Uniprot | |
| K283 | Sumoylation | Uniprot | |
| T298 | Phosphorylation | Uniprot | |
| K450 | Ubiquitination | Uniprot | |
| K468 | Ubiquitination | Uniprot | |
| K556 | Sumoylation | Uniprot | |
| S561 | Phosphorylation | Uniprot | |
| K576 | Ubiquitination | Uniprot | |
| T594 | Phosphorylation | Uniprot | |
| S599 | Phosphorylation | Uniprot | |
| S600 | Phosphorylation | Uniprot | |
| S646 | Phosphorylation | Uniprot | |
| S652 | Phosphorylation | Uniprot | |
| S684 | Phosphorylation | Uniprot | |
| S1197 | Phosphorylation | Q13315 (ATM) | Uniprot |
| S1247 | Phosphorylation | Q13315 (ATM) | Uniprot |
| S1367 | Phosphorylation | Q13315 (ATM) | Uniprot |
研究背景
Transcription factor involved, among others, in the transcriptional regulation of osmoprotective and inflammatory genes. Mediates the transcriptional response to hypertonicity. Positively regulates the transcription of LCN2 and S100A4 genes; optimal transactivation of these genes requires the presence of DDX5/DDX17. Binds the DNA consensus sequence 5'-[ACT][AG]TGGAAA[CAT]A[TA][ATC][CA][ATG][GT][GAC][CG][CT]-3'.
Phosphorylated. Phosphorylated at Thr-135 by CDK5 in response to osmotic stress; this phosphorylation mediates its rapid nuclear localization.
Nucleus. Cytoplasm.
Note: Nuclear distribution increases under hypertonic conditions.
Widely expressed, with highest levels in skeletal muscle, brain, heart and peripheral blood leukocytes.
Homodimer when bound to DNA, completely encircles its DNA target. Interacts with CIDEC; this interaction is direct and retains NFAT5 in the cytoplasm (By similarity). Does not bind with Fos and Jun transcription factors. Interacts with DDX5 and DDX17; this interaction leads to DDX5/DDX17 recruitment to LNC2 and S100A4 promoters and NFAT5-mediated DDX5/DDX17-enhanced transactivation.
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